Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate

In this paper, there were 2 main objectives. Those were to To determine the potential of delignification rice straw by using B.cereus and to study the PHB production from B.cereus by using rice straw as substrate. Delignification was crutial due to its chemical durability makes it indigestible to or...

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Main Author:	Nor Syafarah, Zakariya
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3429/ http://umpir.ump.edu.my/id/eprint/3429/ http://umpir.ump.edu.my/id/eprint/3429/1/CD5959_NOR_SYAFARAH_BITI_ZAKARIYA.pdf

id	ump-3429
recordtype	eprints
spelling	ump-34292015-03-03T08:01:06Z http://umpir.ump.edu.my/id/eprint/3429/ Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate Nor Syafarah, Zakariya TP Chemical technology In this paper, there were 2 main objectives. Those were to To determine the potential of delignification rice straw by using B.cereus and to study the PHB production from B.cereus by using rice straw as substrate. Delignification was crutial due to its chemical durability makes it indigestible to organisms because of its bonding to cellulose and protein material. This lignin sheet acts as a barrier towards the outside elements (Carraher, 2008). The method used for delignification process was fermentation of B.cereus with 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO4.7H 0 and 10 g of rice straw in a 500 mL shake flask. Then, the bacteria were fermented in the medium at 30 ºC and 250 rpm. For the analysis of delignification of rice straw by B.cereus, Klason’s method was utilized. From the result obtained, the highest lignin content was 4.56 % and the Control was 8.18 %. The percentage of the highest lignin degradation for the three samples was 98 %. The average lignin degradation was 78.67 %. After delignification, the bacteria could reacted on the cellulose content to synthesis PHB. The bacteria was fermented in 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO24.7H0 and 1 g of delignified rice straw in a 500 mL shake flask. Then, the bacteria was fermented in the medium at 30 ºC and 250 rpm. The method of analysis of PHB yield was by using UV-vi spectrophotometer at 238nm. From the results obtained, the highest PHB yield is produced in Medium B; the medium consisted of MgSO24.7HO and yeast that yield 2.62 %. By these results, the objectives were achieved. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3429/1/CD5959_NOR_SYAFARAH_BITI_ZAKARIYA.pdf Nor Syafarah, Zakariya (2010) Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:58533&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nor Syafarah, Zakariya Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
description	In this paper, there were 2 main objectives. Those were to To determine the potential of delignification rice straw by using B.cereus and to study the PHB production from B.cereus by using rice straw as substrate. Delignification was crutial due to its chemical durability makes it indigestible to organisms because of its bonding to cellulose and protein material. This lignin sheet acts as a barrier towards the outside elements (Carraher, 2008). The method used for delignification process was fermentation of B.cereus with 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO4.7H 0 and 10 g of rice straw in a 500 mL shake flask. Then, the bacteria were fermented in the medium at 30 ºC and 250 rpm. For the analysis of delignification of rice straw by B.cereus, Klason’s method was utilized. From the result obtained, the highest lignin content was 4.56 % and the Control was 8.18 %. The percentage of the highest lignin degradation for the three samples was 98 %. The average lignin degradation was 78.67 %. After delignification, the bacteria could reacted on the cellulose content to synthesis PHB. The bacteria was fermented in 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO24.7H0 and 1 g of delignified rice straw in a 500 mL shake flask. Then, the bacteria was fermented in the medium at 30 ºC and 250 rpm. The method of analysis of PHB yield was by using UV-vi spectrophotometer at 238nm. From the results obtained, the highest PHB yield is produced in Medium B; the medium consisted of MgSO24.7HO and yeast that yield 2.62 %. By these results, the objectives were achieved.
format	Undergraduates Project Papers
author	Nor Syafarah, Zakariya
author_facet	Nor Syafarah, Zakariya
author_sort	Nor Syafarah, Zakariya
title	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_short	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_full	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_fullStr	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_full_unstemmed	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_sort	production of polyhydroxybutyrate (phb) from bacillus cereus by using rice straw as substrate
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3429/ http://umpir.ump.edu.my/id/eprint/3429/ http://umpir.ump.edu.my/id/eprint/3429/1/CD5959_NOR_SYAFARAH_BITI_ZAKARIYA.pdf
first_indexed	2023-09-18T21:57:43Z
last_indexed	2023-09-18T21:57:43Z
_version_	1777414191436529664

Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate

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