Extraction of papain enzyme from papaya leaves using enzyme assisted method

Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase...

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Main Author:	Roswani, Shaimi
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3323/ http://umpir.ump.edu.my/id/eprint/3323/1/CD5512_ROSWANI_SHAIMI_i.pdf

id	ump-3323
recordtype	eprints
spelling	ump-33232015-03-03T08:00:33Z http://umpir.ump.edu.my/id/eprint/3323/ Extraction of papain enzyme from papaya leaves using enzyme assisted method Roswani, Shaimi TP Chemical technology Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase assisted extraction process was optimized by varying different parameters such as pH of extraction process, Cellulase concentration, Solid to Liquid ratio, incubation time and incubation temperature. Each 5 g of papaya leaves were ground and mixed with acetate buffer at a different Solid to Liquid ratio (1:5 - 1:25 g/ml) and adjusted with different pH (3-8). Cellulase was quantified and dispersed in acetate buffer (Na2HPO—citric acid) in different concentration (0.5 - 2.5 mg/ml).The enzymatic pretreatment was carried out and continued for enzyme assisted extraction process for various temperature and time (5070o4C and 1-5 h respectively). The optimal extraction conditions that satisfied the above constraints were found to be at pH 7, 1.5 mg/ml of Cellulase, solid to liquid of 1:10 (g/ml), an extraction time of 4 h and at an extraction temperature of 65oC. Under the optimum conditions, the extraction yield of papain was successfully achieved as much as 3.8018 µmole with 2.0910 Units/ml in Cellulase treated sample. Therefore, the potential for the papain enzyme in medical field can be fulfilled using enzyme assisted extraction. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3323/1/CD5512_ROSWANI_SHAIMI_i.pdf Roswani, Shaimi (2010) Extraction of papain enzyme from papaya leaves using enzyme assisted method. Faculty of Chemical and Natural Resources Engineering , Universiti Malaysia Pahang .
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Roswani, Shaimi Extraction of papain enzyme from papaya leaves using enzyme assisted method
description	Papain is highly appreciated in medical field that prevents several chronic diseases such as cardiovascular disease, cancer and diabetes. Therefore, the objective of the research is to extract papain enzyme from papaya leaves using enzyme assisted method and determine papain activity. The Cellullase assisted extraction process was optimized by varying different parameters such as pH of extraction process, Cellulase concentration, Solid to Liquid ratio, incubation time and incubation temperature. Each 5 g of papaya leaves were ground and mixed with acetate buffer at a different Solid to Liquid ratio (1:5 - 1:25 g/ml) and adjusted with different pH (3-8). Cellulase was quantified and dispersed in acetate buffer (Na2HPO—citric acid) in different concentration (0.5 - 2.5 mg/ml).The enzymatic pretreatment was carried out and continued for enzyme assisted extraction process for various temperature and time (5070o4C and 1-5 h respectively). The optimal extraction conditions that satisfied the above constraints were found to be at pH 7, 1.5 mg/ml of Cellulase, solid to liquid of 1:10 (g/ml), an extraction time of 4 h and at an extraction temperature of 65oC. Under the optimum conditions, the extraction yield of papain was successfully achieved as much as 3.8018 µmole with 2.0910 Units/ml in Cellulase treated sample. Therefore, the potential for the papain enzyme in medical field can be fulfilled using enzyme assisted extraction.
format	Undergraduates Project Papers
author	Roswani, Shaimi
author_facet	Roswani, Shaimi
author_sort	Roswani, Shaimi
title	Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_short	Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_full	Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_fullStr	Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_full_unstemmed	Extraction of papain enzyme from papaya leaves using enzyme assisted method
title_sort	extraction of papain enzyme from papaya leaves using enzyme assisted method
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3323/ http://umpir.ump.edu.my/id/eprint/3323/1/CD5512_ROSWANI_SHAIMI_i.pdf
first_indexed	2023-09-18T21:57:32Z
last_indexed	2023-09-18T21:57:32Z
_version_	1777414179351691264

Extraction of papain enzyme from papaya leaves using enzyme assisted method

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