Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate

The objectives of this research are to determine the production of lignin degradation in sawdust using Bacillus cereus and to study the potential of Bacillus cereus for PHB production using sawdust as a substrate. There consist of two major parts which are fermentation for delignification of sawdust...

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Main Author:	Farizah, Hamid
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3260/ http://umpir.ump.edu.my/id/eprint/3260/1/CD5838_FAZIRAH_HAMID.pdf

id	ump-3260
recordtype	eprints
spelling	ump-32602015-03-03T08:00:13Z http://umpir.ump.edu.my/id/eprint/3260/ Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate Farizah, Hamid TP Chemical technology The objectives of this research are to determine the production of lignin degradation in sawdust using Bacillus cereus and to study the potential of Bacillus cereus for PHB production using sawdust as a substrate. There consist of two major parts which are fermentation for delignification of sawdust and production of PHB. For delignification analysis, Klason method is used in order to determine the percentage of lignin content and lignin degrades by using sawdust as a substrate. Bacillus cereus act to degrade lignin in the sample. Based on the experiment sample 1 shows the lower percent of lignin content with 38.45% and become the higher percentage of lignin degrade with 13.75%. This is because the bacteria which are Bacillus cereus break down the long carbohydrate chains or hemicellulose converted the sawdust into short chains of glucose and eventually individual glucose. While sample 4 shows the lowest percentage of lignin degrade but higher percentage of lignin content. The second part is on the production of PHB. From the result, percentage yield of PHB produced from cellulose and glucose are 1.29% and 3.42%. In conclusion, Bacillus cereus has potential to produce PHB using sawdust as a substrate although the yield is smaller. Several precautions must take placed to avoid any mistaken during the experiment. As a recommendation HPLC can be used to obtain more accurate result. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3260/1/CD5838_FAZIRAH_HAMID.pdf Farizah, Hamid (2010) Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang .
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Farizah, Hamid Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate
description	The objectives of this research are to determine the production of lignin degradation in sawdust using Bacillus cereus and to study the potential of Bacillus cereus for PHB production using sawdust as a substrate. There consist of two major parts which are fermentation for delignification of sawdust and production of PHB. For delignification analysis, Klason method is used in order to determine the percentage of lignin content and lignin degrades by using sawdust as a substrate. Bacillus cereus act to degrade lignin in the sample. Based on the experiment sample 1 shows the lower percent of lignin content with 38.45% and become the higher percentage of lignin degrade with 13.75%. This is because the bacteria which are Bacillus cereus break down the long carbohydrate chains or hemicellulose converted the sawdust into short chains of glucose and eventually individual glucose. While sample 4 shows the lowest percentage of lignin degrade but higher percentage of lignin content. The second part is on the production of PHB. From the result, percentage yield of PHB produced from cellulose and glucose are 1.29% and 3.42%. In conclusion, Bacillus cereus has potential to produce PHB using sawdust as a substrate although the yield is smaller. Several precautions must take placed to avoid any mistaken during the experiment. As a recommendation HPLC can be used to obtain more accurate result.
format	Undergraduates Project Papers
author	Farizah, Hamid
author_facet	Farizah, Hamid
author_sort	Farizah, Hamid
title	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate
title_short	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate
title_full	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate
title_fullStr	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate
title_full_unstemmed	Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate
title_sort	production of polyhydroxybutyrate (phb) from bacillus cereus by using sawdust as a substrate
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3260/ http://umpir.ump.edu.my/id/eprint/3260/1/CD5838_FAZIRAH_HAMID.pdf
first_indexed	2023-09-18T21:57:24Z
last_indexed	2023-09-18T21:57:24Z
_version_	1777414171015512064

Production of polyhydroxybutyrate (PHB) from bacillus cereus by using sawdust as a substrate

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