Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)

By now, the importance of inoculums development was well appreciated in bioprocessing. Inoculums development receives as much care and attention as the growth and product cycles. The status or “health” of the inoculums has a great deal to do with the success of the production cycle. In this project,...

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Main Author:	Leong, Choon Wei
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3191/ http://umpir.ump.edu.my/id/eprint/3191/ http://umpir.ump.edu.my/id/eprint/3191/1/CD5601_LEONG_CHOON_WEI.pdf

id	ump-3191
recordtype	eprints
spelling	ump-31912015-03-03T07:59:56Z http://umpir.ump.edu.my/id/eprint/3191/ Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah) Leong, Choon Wei TP Chemical technology By now, the importance of inoculums development was well appreciated in bioprocessing. Inoculums development receives as much care and attention as the growth and product cycles. The status or “health” of the inoculums has a great deal to do with the success of the production cycle. In this project, inoculums development for the production of monoclonal antibody against Congenital Adrenal Hyperplasia (CAH) was studied. The scopes focused were the age of inoculums (passage number) and inoculums density at different growth phases. A hybridoma cell line (Hybridoma 192) was cultivated in batch mode with varying inoculums cell densities and growth phases using Dulbecco’s Modified Eagle Medium (DMEM). The maximum viable cell densities and the average specific MAb production rate (rp) were found to dependent on the inoculums cell density and the growth phases. Inoculums with low cell density at 5X104cells/ml and early log phase (t=28h) have the highest average specific MAb production rate but a low maximum viable cell density. Varying inoculums cell densites and growth phases did not affect the specific growth rate, maximum MAb titer and net production of MAb. Since the inoculums density in the range of 5x104 cells/ml to 5x105 cells/ml and growth phase do not affect the net production of MAb, it is wise to use high initial inoculums cell density at 5x105 cells/ml because the cell growth easily and faster compare low inoculums density. For the inoculums age, the average specific MAb production rate decreased when the passage number increased from PN (Passage Number) =9 to PN=30.Subculture exceeding critical passage number 9 was thus not recommended. 2010-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3191/1/CD5601_LEONG_CHOON_WEI.pdf Leong, Choon Wei (2010) Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah). Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang . http://iportal.ump.edu.my/lib/item?id=chamo:58166&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Leong, Choon Wei Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
description	By now, the importance of inoculums development was well appreciated in bioprocessing. Inoculums development receives as much care and attention as the growth and product cycles. The status or “health” of the inoculums has a great deal to do with the success of the production cycle. In this project, inoculums development for the production of monoclonal antibody against Congenital Adrenal Hyperplasia (CAH) was studied. The scopes focused were the age of inoculums (passage number) and inoculums density at different growth phases. A hybridoma cell line (Hybridoma 192) was cultivated in batch mode with varying inoculums cell densities and growth phases using Dulbecco’s Modified Eagle Medium (DMEM). The maximum viable cell densities and the average specific MAb production rate (rp) were found to dependent on the inoculums cell density and the growth phases. Inoculums with low cell density at 5X104cells/ml and early log phase (t=28h) have the highest average specific MAb production rate but a low maximum viable cell density. Varying inoculums cell densites and growth phases did not affect the specific growth rate, maximum MAb titer and net production of MAb. Since the inoculums density in the range of 5x104 cells/ml to 5x105 cells/ml and growth phase do not affect the net production of MAb, it is wise to use high initial inoculums cell density at 5x105 cells/ml because the cell growth easily and faster compare low inoculums density. For the inoculums age, the average specific MAb production rate decreased when the passage number increased from PN (Passage Number) =9 to PN=30.Subculture exceeding critical passage number 9 was thus not recommended.
format	Undergraduates Project Papers
author	Leong, Choon Wei
author_facet	Leong, Choon Wei
author_sort	Leong, Choon Wei
title	Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
title_short	Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
title_full	Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
title_fullStr	Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
title_full_unstemmed	Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
title_sort	inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3191/ http://umpir.ump.edu.my/id/eprint/3191/ http://umpir.ump.edu.my/id/eprint/3191/1/CD5601_LEONG_CHOON_WEI.pdf
first_indexed	2023-09-18T21:57:18Z
last_indexed	2023-09-18T21:57:18Z
_version_	1777414165182283776

Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)

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