Inoculums development for the production of monoclonal antibody against congenital adrenal hyperplasia(cah)
By now, the importance of inoculums development was well appreciated in bioprocessing. Inoculums development receives as much care and attention as the growth and product cycles. The status or “health” of the inoculums has a great deal to do with the success of the production cycle. In this project,...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2010
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| Online Access: | http://umpir.ump.edu.my/id/eprint/3191/ http://umpir.ump.edu.my/id/eprint/3191/ http://umpir.ump.edu.my/id/eprint/3191/1/CD5601_LEONG_CHOON_WEI.pdf |
| Summary: | By now, the importance of inoculums development was well appreciated in bioprocessing. Inoculums development receives as much care and attention as the growth and product cycles. The status or “health” of the inoculums has a great deal to do with the success of the production cycle. In this project, inoculums development for the production of monoclonal antibody against Congenital Adrenal Hyperplasia (CAH) was studied. The scopes focused were the age of inoculums (passage number) and inoculums density at different growth phases. A hybridoma cell line (Hybridoma 192) was cultivated in batch mode with varying inoculums cell densities and growth phases using Dulbecco’s Modified Eagle Medium (DMEM). The maximum viable cell densities and the average specific MAb production rate (rp) were found to dependent on the inoculums cell density and the growth phases. Inoculums with low cell density at 5X104cells/ml and early log phase (t=28h) have the highest average specific MAb production rate but a low maximum viable cell density. Varying inoculums cell densites and growth phases did not affect the specific growth rate, maximum MAb titer and net production of MAb. Since the inoculums density in the range of 5x104 cells/ml to 5x105 cells/ml and growth phase do not affect the net production of MAb, it is wise to use high initial inoculums cell density at 5x105 cells/ml because the cell growth easily and faster compare low inoculums density. For the inoculums age, the average specific MAb production rate decreased when the passage number increased from PN (Passage Number) =9 to PN=30.Subculture exceeding critical passage number 9 was thus not recommended. |
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