Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells

Onion (Allium cepa) and leek (Allium ampeloprasum var. porrum) are common herbs and vegetables found in our daily life. It belongs to the genus Allium, which is usually known for their high antioxidant and anticancer properties. Medical researchers highly recommend the exploitation of herbs and plan...

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Main Authors: Normaiza, Z., Hazrulrizawati, Hamid
Format: Article
Language:English
Published: Springer 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/27950/
http://umpir.ump.edu.my/id/eprint/27950/
http://umpir.ump.edu.my/id/eprint/27950/
http://umpir.ump.edu.my/id/eprint/27950/1/Comparative%20study%20of%20onion%20%28Allium%20cepa%29%20and%20leek%20.pdf
id ump-27950
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spelling ump-279502020-02-26T04:16:56Z http://umpir.ump.edu.my/id/eprint/27950/ Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells Normaiza, Z. Hazrulrizawati, Hamid QD Chemistry TP Chemical technology Onion (Allium cepa) and leek (Allium ampeloprasum var. porrum) are common herbs and vegetables found in our daily life. It belongs to the genus Allium, which is usually known for their high antioxidant and anticancer properties. Medical researchers highly recommend the exploitation of herbs and plants as alternative ways in the treatment of cancer. This research was designed to study the anticancer effects of onion and leek extracts on MCF-7 human breast cancer cell. Crude extracts of ethanol, methanol, and water of onion and leek were obtained by maceration. MCF-7 cells were cultured in complete media at 37 °C and subjected to different treatments that involved varying concentrations (10, 50, and 100 μg/mL) of onion and leek extracts for 24, 48, and 72 h of incubation. The percentage of cell viability and the concentration of extracts on MCF-7 cells were determined using MTT assay. The water leek extract proved to be the most effective extract at 50 μg/mL, whereby it showed a significant inhibition ability due to the presence of entadamide A-β-D-glucopyranoside as identified by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF/MS). Further studies about the mechanism of both extracts in causing cell death and the determination of the presence of other bioactive compounds in the extracts are needed. Springer 2019-12-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/27950/1/Comparative%20study%20of%20onion%20%28Allium%20cepa%29%20and%20leek%20.pdf Normaiza, Z. and Hazrulrizawati, Hamid (2019) Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells. Plant Foods for Human Nutrition, 74 (4). pp. 525-530. ISSN 1573-9104 https://doi.org/10.1007/s11130-019-00770-6 https://doi.org/10.1007/s11130-019-00770-6
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QD Chemistry
TP Chemical technology
spellingShingle QD Chemistry
TP Chemical technology
Normaiza, Z.
Hazrulrizawati, Hamid
Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells
description Onion (Allium cepa) and leek (Allium ampeloprasum var. porrum) are common herbs and vegetables found in our daily life. It belongs to the genus Allium, which is usually known for their high antioxidant and anticancer properties. Medical researchers highly recommend the exploitation of herbs and plants as alternative ways in the treatment of cancer. This research was designed to study the anticancer effects of onion and leek extracts on MCF-7 human breast cancer cell. Crude extracts of ethanol, methanol, and water of onion and leek were obtained by maceration. MCF-7 cells were cultured in complete media at 37 °C and subjected to different treatments that involved varying concentrations (10, 50, and 100 μg/mL) of onion and leek extracts for 24, 48, and 72 h of incubation. The percentage of cell viability and the concentration of extracts on MCF-7 cells were determined using MTT assay. The water leek extract proved to be the most effective extract at 50 μg/mL, whereby it showed a significant inhibition ability due to the presence of entadamide A-β-D-glucopyranoside as identified by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF/MS). Further studies about the mechanism of both extracts in causing cell death and the determination of the presence of other bioactive compounds in the extracts are needed.
format Article
author Normaiza, Z.
Hazrulrizawati, Hamid
author_facet Normaiza, Z.
Hazrulrizawati, Hamid
author_sort Normaiza, Z.
title Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells
title_short Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells
title_full Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells
title_fullStr Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells
title_full_unstemmed Comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by UPLC-QTOF/MS and anticancer effect on MCF-7 cells
title_sort comparative study of onion (allium cepa) and leek (allium ampeloprasum) : identification of organosulphur compounds by uplc-qtof/ms and anticancer effect on mcf-7 cells
publisher Springer
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/27950/
http://umpir.ump.edu.my/id/eprint/27950/
http://umpir.ump.edu.my/id/eprint/27950/
http://umpir.ump.edu.my/id/eprint/27950/1/Comparative%20study%20of%20onion%20%28Allium%20cepa%29%20and%20leek%20.pdf
first_indexed 2023-09-18T22:43:49Z
last_indexed 2023-09-18T22:43:49Z
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