Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children

Introduction: Mucosal-associated invariant T (MAIT) cells are unconventional T cells, enriched in the gut. They express an invariant T-cell receptor and recognize riboflavin metabolites from bacteria presented by MHC-Ib-related protein 1 (MR1) molecules. Alterations in gut microbiota have been rep...

Full description

Bibliographic Details
Main Authors:	Ahmad Mahfuz, Gazali, Näntö-Salonen, Kirsti, Rintamäki, Reeta, Pihlajamäki, Jussi, Knip, Mikael, Veijola, Riitta, Toppari, Jorma, Ilonen, Jorma, Kinnunen, Tuure
Format:	Article
Language:	English
Published:	Universiti Putra Malaysia 2019
Subjects:	Q Science (General) RZ Other systems of medicine
Online Access:	http://umpir.ump.edu.my/id/eprint/27699/ http://umpir.ump.edu.my/id/eprint/27699/ http://umpir.ump.edu.my/id/eprint/27699/7/Altered%20ICOMOI2019.pdf

id	ump-27699
recordtype	eprints
spelling	ump-276992020-02-12T02:28:43Z http://umpir.ump.edu.my/id/eprint/27699/ Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children Ahmad Mahfuz, Gazali Näntö-Salonen, Kirsti Rintamäki, Reeta Pihlajamäki, Jussi Knip, Mikael Veijola, Riitta Toppari, Jorma Ilonen, Jorma Kinnunen, Tuure Q Science (General) RZ Other systems of medicine Introduction: Mucosal-associated invariant T (MAIT) cells are unconventional T cells, enriched in the gut. They express an invariant T-cell receptor and recognize riboflavin metabolites from bacteria presented by MHC-Ib-related protein 1 (MR1) molecules. Alterations in gut microbiota have been reported in patients with type 1 diabetes (T1D), even before the onset of the disease. These changes can potentially alter the frequency or phenotype of circulating MAIT cells. Methods: We characterized peripheral blood MAIT cells in a cohort of 51 children with newly diagnosed T1D, 27 at-risk children positive for multiple autoantibodies (AAb+) and 113 age-matched healthy children. Using multi-colour flow cytometry, we analysed the frequency, surface phenotype and cytokine production of MAIT cells. In addition, we characterized the frequency and surface phenotype of blood MAIT cells in 26 patients with long-standing T1D and 25 age-matched healthy controls. Results: No significant differences in MAIT cell frequency were observed between the study groups. Further phenotyping revealed that the expression of CD8, CD27, CCR5 and β7 integrin on MAIT cells was lower in children with newly diagnosed T1D compared to AAb+ and healthy children. The frequency of MAIT cells producing IFN-γ was also lower in children with newly diagnosed T1D, but the frequencies of IL-17A- and IL-4-secreting MAIT cells were similar in the study groups. Finally, the capacity of MAIT cells to be activated in vitro by E.coli bacteria through MR1 was comparable between the study groups. However, none of these changes was observed in adult patients with long-standing T1D. In contrast, a decreased frequency of MAIT cells and increased CD25 expression was observed in adult T1D patients with a short duration after diagnosis. Conclusion: There are subtle changes in the circulating MAIT compartment in patients with T1D at the onset of the disease as well as after clinical diagnosis, but not in AAb+ at-risk subjects including progression to clinical disease. Consequently, the alterations in blood MAIT cells are likely associated with the clinical manifestation of the disease rather than being features of earlier T1D autoimmunity. Universiti Putra Malaysia 2019-11-29 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/27699/7/Altered%20ICOMOI2019.pdf Ahmad Mahfuz, Gazali and Näntö-Salonen, Kirsti and Rintamäki, Reeta and Pihlajamäki, Jussi and Knip, Mikael and Veijola, Riitta and Toppari, Jorma and Ilonen, Jorma and Kinnunen, Tuure (2019) Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children. Malaysian Journal of Medicine and Health Sciences: Proceedings of the 2nd ICOMOI 2019, 15 (Supplement 8). p. 19. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/20191129102937Complete_file_2nd_ICOMOI_2019.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) RZ Other systems of medicine
spellingShingle	Q Science (General) RZ Other systems of medicine Ahmad Mahfuz, Gazali Näntö-Salonen, Kirsti Rintamäki, Reeta Pihlajamäki, Jussi Knip, Mikael Veijola, Riitta Toppari, Jorma Ilonen, Jorma Kinnunen, Tuure Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children
description	Introduction: Mucosal-associated invariant T (MAIT) cells are unconventional T cells, enriched in the gut. They express an invariant T-cell receptor and recognize riboflavin metabolites from bacteria presented by MHC-Ib-related protein 1 (MR1) molecules. Alterations in gut microbiota have been reported in patients with type 1 diabetes (T1D), even before the onset of the disease. These changes can potentially alter the frequency or phenotype of circulating MAIT cells. Methods: We characterized peripheral blood MAIT cells in a cohort of 51 children with newly diagnosed T1D, 27 at-risk children positive for multiple autoantibodies (AAb+) and 113 age-matched healthy children. Using multi-colour flow cytometry, we analysed the frequency, surface phenotype and cytokine production of MAIT cells. In addition, we characterized the frequency and surface phenotype of blood MAIT cells in 26 patients with long-standing T1D and 25 age-matched healthy controls. Results: No significant differences in MAIT cell frequency were observed between the study groups. Further phenotyping revealed that the expression of CD8, CD27, CCR5 and β7 integrin on MAIT cells was lower in children with newly diagnosed T1D compared to AAb+ and healthy children. The frequency of MAIT cells producing IFN-γ was also lower in children with newly diagnosed T1D, but the frequencies of IL-17A- and IL-4-secreting MAIT cells were similar in the study groups. Finally, the capacity of MAIT cells to be activated in vitro by E.coli bacteria through MR1 was comparable between the study groups. However, none of these changes was observed in adult patients with long-standing T1D. In contrast, a decreased frequency of MAIT cells and increased CD25 expression was observed in adult T1D patients with a short duration after diagnosis. Conclusion: There are subtle changes in the circulating MAIT compartment in patients with T1D at the onset of the disease as well as after clinical diagnosis, but not in AAb+ at-risk subjects including progression to clinical disease. Consequently, the alterations in blood MAIT cells are likely associated with the clinical manifestation of the disease rather than being features of earlier T1D autoimmunity.
format	Article
author	Ahmad Mahfuz, Gazali Näntö-Salonen, Kirsti Rintamäki, Reeta Pihlajamäki, Jussi Knip, Mikael Veijola, Riitta Toppari, Jorma Ilonen, Jorma Kinnunen, Tuure
author_facet	Ahmad Mahfuz, Gazali Näntö-Salonen, Kirsti Rintamäki, Reeta Pihlajamäki, Jussi Knip, Mikael Veijola, Riitta Toppari, Jorma Ilonen, Jorma Kinnunen, Tuure
author_sort	Ahmad Mahfuz, Gazali
title	Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children
title_short	Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children
title_full	Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children
title_fullStr	Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children
title_full_unstemmed	Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children
title_sort	altered mucosal-associated invariant t cells phenotype in children with newly diagnosed type 1 diabetes but not in autoantibody-positive at-risk children
publisher	Universiti Putra Malaysia
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/27699/ http://umpir.ump.edu.my/id/eprint/27699/ http://umpir.ump.edu.my/id/eprint/27699/7/Altered%20ICOMOI2019.pdf
first_indexed	2023-09-18T22:43:30Z
last_indexed	2023-09-18T22:43:30Z
_version_	1777417071864315904

Altered Mucosal-Associated Invariant T Cells Phenotype in Children with Newly Diagnosed Type 1 Diabetes but not in Autoantibody-Positive At-Risk Children

Similar Items