Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots

Even though Eurycoma longifolia was extensively studied, few researches investigated the protein content in its water extracts with the consideration of establishing the most suitable extraction method to increase their yields with high efficiency and less time consumption. The study applied a seque...

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Main Authors:	Elhag, Husam Eldin Elhag Abugabr, Ahmad Ziad, Sulaiman, Azilah, Ajit
Format:	Conference or Workshop Item
Language:	English English
Published:	Universiti Malaysia Pahang 2019
Subjects:	TD Environmental technology. Sanitary engineering
Online Access:	http://umpir.ump.edu.my/id/eprint/27648/ http://umpir.ump.edu.my/id/eprint/27648/1/9.%20Sequential%20ultrasound-microwave%20assisted%20extraction%20of%20water.pdf http://umpir.ump.edu.my/id/eprint/27648/2/9.1%20Sequential%20ultrasound-microwave%20assisted%20extraction%20of%20water.pdf

id	ump-27648
recordtype	eprints
spelling	ump-276482020-02-07T07:33:02Z http://umpir.ump.edu.my/id/eprint/27648/ Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots Elhag, Husam Eldin Elhag Abugabr Ahmad Ziad, Sulaiman Azilah, Ajit TD Environmental technology. Sanitary engineering Even though Eurycoma longifolia was extensively studied, few researches investigated the protein content in its water extracts with the consideration of establishing the most suitable extraction method to increase their yields with high efficiency and less time consumption. The study applied a sequential extraction method to increase the yields of water soluble proteins (WSPs) in E. longifolia root extracts by the application of two nonconventional extraction methods, Microwave assisted extraction (MAE) and ultrasound assisted extraction (UAE). The study was established by circumscribed central composite designs (CCCDs) to indicate the optimum extraction conditions and the corresponding maximum WSPs yields for both the methods by investigating the MAE factors which were temperature (T); microwave power (W) and irradiation time (i) and UAE factors temperature (T); ultrasonic intensity (UI); and sonication time (s). The optimum conditions of MAE (T: 54oC, W: 301W and i: 15 min) led to the WSPs yield 23.101±1.647% .The optimum conditions of UAE (T: 46oC; UI: 1.84 W.cm-2 and s: 24 min) led to 24.181±0.321%. These predicted optimum conditions were then employed for the two proposed sequential extraction method, sequential ultrasound-microwave assisted extraction (SUMAE) and sequential microwave-ultrasound assisted extraction (SMUAE). Results revealed that the highest WSPs yield (27.172±1.086%) was obtained by initiating the sequential extraction with UAE for 10 minutes followed by MAE treatment for 5 minutes was the best extraction process. Therefore, SUMAE illustrated more efficiency than SMUAE. This concluded that the SUMAE is a more efficient extraction process than the one-step nonconventional extraction methods and was nominated for the upscaling of extracting proteins from E. longifolia roots in pilot and industrial scales. Universiti Malaysia Pahang 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/27648/1/9.%20Sequential%20ultrasound-microwave%20assisted%20extraction%20of%20water.pdf pdf en http://umpir.ump.edu.my/id/eprint/27648/2/9.1%20Sequential%20ultrasound-microwave%20assisted%20extraction%20of%20water.pdf Elhag, Husam Eldin Elhag Abugabr and Ahmad Ziad, Sulaiman and Azilah, Ajit (2019) Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots. In: 11th Malaysian Technical Universities Conference on Engineering and Technology (MUCET 2019), 19-20 November 2019 , Kuantan, Pahang. pp. 1-14.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TD Environmental technology. Sanitary engineering
spellingShingle	TD Environmental technology. Sanitary engineering Elhag, Husam Eldin Elhag Abugabr Ahmad Ziad, Sulaiman Azilah, Ajit Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots
description	Even though Eurycoma longifolia was extensively studied, few researches investigated the protein content in its water extracts with the consideration of establishing the most suitable extraction method to increase their yields with high efficiency and less time consumption. The study applied a sequential extraction method to increase the yields of water soluble proteins (WSPs) in E. longifolia root extracts by the application of two nonconventional extraction methods, Microwave assisted extraction (MAE) and ultrasound assisted extraction (UAE). The study was established by circumscribed central composite designs (CCCDs) to indicate the optimum extraction conditions and the corresponding maximum WSPs yields for both the methods by investigating the MAE factors which were temperature (T); microwave power (W) and irradiation time (i) and UAE factors temperature (T); ultrasonic intensity (UI); and sonication time (s). The optimum conditions of MAE (T: 54oC, W: 301W and i: 15 min) led to the WSPs yield 23.101±1.647% .The optimum conditions of UAE (T: 46oC; UI: 1.84 W.cm-2 and s: 24 min) led to 24.181±0.321%. These predicted optimum conditions were then employed for the two proposed sequential extraction method, sequential ultrasound-microwave assisted extraction (SUMAE) and sequential microwave-ultrasound assisted extraction (SMUAE). Results revealed that the highest WSPs yield (27.172±1.086%) was obtained by initiating the sequential extraction with UAE for 10 minutes followed by MAE treatment for 5 minutes was the best extraction process. Therefore, SUMAE illustrated more efficiency than SMUAE. This concluded that the SUMAE is a more efficient extraction process than the one-step nonconventional extraction methods and was nominated for the upscaling of extracting proteins from E. longifolia roots in pilot and industrial scales.
format	Conference or Workshop Item
author	Elhag, Husam Eldin Elhag Abugabr Ahmad Ziad, Sulaiman Azilah, Ajit
author_facet	Elhag, Husam Eldin Elhag Abugabr Ahmad Ziad, Sulaiman Azilah, Ajit
author_sort	Elhag, Husam Eldin Elhag Abugabr
title	Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots
title_short	Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots
title_full	Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots
title_fullStr	Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots
title_full_unstemmed	Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots
title_sort	sequential ultrasound-microwave assisted extraction of water soluble proteins from eurycoma longifolia roots
publisher	Universiti Malaysia Pahang
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/27648/ http://umpir.ump.edu.my/id/eprint/27648/1/9.%20Sequential%20ultrasound-microwave%20assisted%20extraction%20of%20water.pdf http://umpir.ump.edu.my/id/eprint/27648/2/9.1%20Sequential%20ultrasound-microwave%20assisted%20extraction%20of%20water.pdf
first_indexed	2023-09-18T22:43:27Z
last_indexed	2023-09-18T22:43:27Z
_version_	1777417068458541056

Sequential ultrasound-microwave assisted extraction of water soluble proteins from Eurycoma longifolia roots

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