Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin

Background: This paper presents the effect of solution properties and operating parameters of polyethylene oxide (PEO) based nanofiber using a wire electrode-based needleless electrospinning. Methods: The feed solution was prepared using a PEO dissolved in water or a water-ethanol mixture. The PEO...

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Main Authors:	Ramakrishnan, Ramprasath, Jolius, Gimbun, Ramakrishnan, Praveena, Ranganathan, Balu, Reddy, Samala Murali Mohan, Ganesh, Shanmugam
Format:	Article
Language:	English
Published:	Bentham Science Publishers 2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/27253/ http://umpir.ump.edu.my/id/eprint/27253/ http://umpir.ump.edu.my/id/eprint/27253/ http://umpir.ump.edu.my/id/eprint/27253/7/Effect%20of%20Solution%20Properties%20and%20Operating%20Parameters1.pdf

id	ump-27253
recordtype	eprints
spelling	ump-272532020-01-23T02:03:00Z http://umpir.ump.edu.my/id/eprint/27253/ Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin Ramakrishnan, Ramprasath Jolius, Gimbun Ramakrishnan, Praveena Ranganathan, Balu Reddy, Samala Murali Mohan Ganesh, Shanmugam TP Chemical technology Background: This paper presents the effect of solution properties and operating parameters of polyethylene oxide (PEO) based nanofiber using a wire electrode-based needleless electrospinning. Methods: The feed solution was prepared using a PEO dissolved in water or a water-ethanol mixture. The PEO solution is blended with Bovine Serum Albumin protein (BSA) as a model drug to study the effect of the electrospinning process on the stability of the loaded protein. The polymer solution properties such as viscosity, surface tension, and conductivity were controlled by adjusting the solvent and salt content. The morphology and fiber size distribution of the nanofiber was analyzed using scanning electron microscopy. Results: The results show that the issue of a beaded nanofiber can be eliminated either by increasing the solution viscosity or by the addition of salt and ethanol to the PEO-water system. The addition of salt and solvent produced a high frequency of smaller fiber diameter ranging from 100 to 150 nm. The encapsulation of BSA in PEO nanofiber was characterized by three different spectroscopy techniques (i.e. circular dichroism, Fourier transform infrared, and fluorescence) and the results showed the BSA is well encapsulated in the PEO matrix with no changes in the protein structure. Conclusion: This work may serve as a useful guide for a drug delivery industry to process a nanofiber at a large and continuous scale with a blend of drugs in nanofiber using a wire electrode electrospinning. Bentham Science Publishers 2019-10 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/27253/7/Effect%20of%20Solution%20Properties%20and%20Operating%20Parameters1.pdf Ramakrishnan, Ramprasath and Jolius, Gimbun and Ramakrishnan, Praveena and Ranganathan, Balu and Reddy, Samala Murali Mohan and Ganesh, Shanmugam (2019) Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin. Current drug delivery, 16 (10). pp. 913-922. ISSN 1567-2018 https://doi.org/10.2174/1567201816666191029122445 https://doi.org/10.2174/1567201816666191029122445
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ramakrishnan, Ramprasath Jolius, Gimbun Ramakrishnan, Praveena Ranganathan, Balu Reddy, Samala Murali Mohan Ganesh, Shanmugam Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin
description	Background: This paper presents the effect of solution properties and operating parameters of polyethylene oxide (PEO) based nanofiber using a wire electrode-based needleless electrospinning. Methods: The feed solution was prepared using a PEO dissolved in water or a water-ethanol mixture. The PEO solution is blended with Bovine Serum Albumin protein (BSA) as a model drug to study the effect of the electrospinning process on the stability of the loaded protein. The polymer solution properties such as viscosity, surface tension, and conductivity were controlled by adjusting the solvent and salt content. The morphology and fiber size distribution of the nanofiber was analyzed using scanning electron microscopy. Results: The results show that the issue of a beaded nanofiber can be eliminated either by increasing the solution viscosity or by the addition of salt and ethanol to the PEO-water system. The addition of salt and solvent produced a high frequency of smaller fiber diameter ranging from 100 to 150 nm. The encapsulation of BSA in PEO nanofiber was characterized by three different spectroscopy techniques (i.e. circular dichroism, Fourier transform infrared, and fluorescence) and the results showed the BSA is well encapsulated in the PEO matrix with no changes in the protein structure. Conclusion: This work may serve as a useful guide for a drug delivery industry to process a nanofiber at a large and continuous scale with a blend of drugs in nanofiber using a wire electrode electrospinning.
format	Article
author	Ramakrishnan, Ramprasath Jolius, Gimbun Ramakrishnan, Praveena Ranganathan, Balu Reddy, Samala Murali Mohan Ganesh, Shanmugam
author_facet	Ramakrishnan, Ramprasath Jolius, Gimbun Ramakrishnan, Praveena Ranganathan, Balu Reddy, Samala Murali Mohan Ganesh, Shanmugam
author_sort	Ramakrishnan, Ramprasath
title	Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin
title_short	Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin
title_full	Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin
title_fullStr	Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin
title_full_unstemmed	Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin
title_sort	effect of solution properties and operating parameters on needleless electrospinning of poly (ethylene oxide) nanofibers loaded with bovine serum albumin
publisher	Bentham Science Publishers
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/27253/ http://umpir.ump.edu.my/id/eprint/27253/ http://umpir.ump.edu.my/id/eprint/27253/ http://umpir.ump.edu.my/id/eprint/27253/7/Effect%20of%20Solution%20Properties%20and%20Operating%20Parameters1.pdf
first_indexed	2023-09-18T22:42:46Z
last_indexed	2023-09-18T22:42:46Z
_version_	1777417025292861440

Effect of Solution Properties and Operating Parameters on Needleless Electrospinning of Poly (Ethylene Oxide) Nanofibers Loaded with Bovine Serum Albumin

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