Fabrication and antimicrobial analysis of composite biodegradable film from pumpkin starch
The purpose of the research is to fabricate difference types of composite biodegradable films from pumpkin starch, to analyze antimicrobial activities of chitosan-incorporated pumpkin starch films and to characterize the biodegradable film (pumpkin starch) in term of physical properties. In this...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/2677/ http://umpir.ump.edu.my/id/eprint/2677/1/AHMAD_BAZLI_BIN_BUSTARY.PDF |
| Summary: | The purpose of the research is to fabricate difference types of composite biodegradable
films from pumpkin starch, to analyze antimicrobial activities of chitosan-incorporated pumpkin
starch films and to characterize the biodegradable film (pumpkin starch) in term of physical
properties. In this study, the pumpkin starch is used as a raw material because of its high
composition of starch. The process involve for this experiment are preparation of pumpkin flour
and then the starch is isolated from the pumpkin flour by mixing with the water and settling it
over night without shaking to recover the sediment as a pumpkin starch. The next process is to
prepare the film by adding the polyethylene glycol (PEG) as plasticizers at a concentration of 3%
and the chitosan was added in a different concentration (1.5g, 2.0g, 2.5g) as preparation of
microbial film and mixing all of component. After being heated the solution, the warm mixture
was casted on framed glass plates, and then dried at a room temperature for 8 hours. Then, the
sample is characterized after the film is produced. The analysis shows that the sample with high
concentration of chitosan has lower water solubility. The percentage of water solubility in each
sample 1.5g, 2.Og and 2.5 g chitosan is 69.7%, 65.2% and 52.3% respectively. The Fourier
transforms infrared spectroscopy (FTIR) result show that the similar pattern of peak at
wavenumbers range 1500 cm -1600 cm-1 with addition of chitosan the peak become narrower
and weaker. Thus, the addition of chitosan can disturb the crystalline of the starch which is the
main factor to determine the characteristic of biodegradable film. The antimicrobial analysis
shows that the Escherichia coil and Bacillus subtilis cannot growth well at the film surface. The
microorganism colonies are smaller in the film with high concentration of chitosan. The scanning
electron microscope (SEM) show that the characteristic features with high concentration of
chitosan has a smooth surface compare to the other sample because of the high bonding between
the molecules in the sample. |
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