Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil

Fenugreek (Trigonella-Foenum Graecum) is known as one of the traditional and most promising medicinal herbs belongs to the leguminous family. The seeds of fenugreek have been extensively studied for the treatment of inflammation, cancer and diabetes. In this study, fenugreek seed oil was extracted a...

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Main Authors:	Akbari, Sweeta, Nour, A. H., R. M., Yunus, Alara, Oluwaseun Ruth, Abayomi, Olalere Olusegun
Format:	Article
Language:	English
Published:	Elsevier Ltd 2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/26644/ http://umpir.ump.edu.my/id/eprint/26644/ http://umpir.ump.edu.my/id/eprint/26644/ http://umpir.ump.edu.my/id/eprint/26644/1/Extraction%2C%20characterization%20and%20antioxidant.pdf

id	ump-26644
recordtype	eprints
spelling	ump-266442020-02-28T07:56:15Z http://umpir.ump.edu.my/id/eprint/26644/ Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil Akbari, Sweeta Nour, A. H. R. M., Yunus Alara, Oluwaseun Ruth Abayomi, Olalere Olusegun TP Chemical technology Fenugreek (Trigonella-Foenum Graecum) is known as one of the traditional and most promising medicinal herbs belongs to the leguminous family. The seeds of fenugreek have been extensively studied for the treatment of inflammation, cancer and diabetes. In this study, fenugreek seed oil was extracted and evaluated for its chemical compositions and bonding through gas chromatography coupled to mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FT-IR) analysis, respectively. The antioxidant activity against 2,2-diphenyl-picrylhydrazyl (DPPH) and 2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS+•) radicals, total phenolic content (TPC) and total flavonoid content (TFC) of the oil were also studied. The capacity of antioxidants detected by ABTS was stronger than that by DPPH. A total number of 23 chemical compounds were detected and identified in fenugreek seed oil comprising of 99% of the total oil through GC-MS analysis. The major compounds of the extracted oil were linoleic acid (54.13%), palmitic acid (16.21%), pinene (4.56%), 4-Pentyl-1-(4-propylcyclohexyl)-1-cyclohexene (3.87%) and linoleic acid methyl ester (3.19%). FTIR analysis confirmed the presence of carboxyl group in the oil which were more dominated by unsaturated essential fatty acids. Moreover, the oil of fenugreek seed indicated a strong antioxidant radical scavenging activity against both DPPH and ABTS assays with an IC50 of 172.6 ± 3.1 and 161.3 ± 2.21, respectively. The TPC and TFC of the oil were 38.97 ± 0.34 mg GAE/g. oil and 14.417 ± 0.23 mg QE/g.oil. Thus, this study suggests that the fenugreek seed oil could be used for pharmaceutical purposes. Elsevier Ltd 2019 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/26644/1/Extraction%2C%20characterization%20and%20antioxidant.pdf Akbari, Sweeta and Nour, A. H. and R. M., Yunus and Alara, Oluwaseun Ruth and Abayomi, Olalere Olusegun (2019) Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil. Materials Science for Energy Technologies, 2 (2). pp. 349-355. ISSN 2589-2991 https://doi.org/10.1016/j.mset.2018.12.001 https://doi.org/10.1016/j.mset.2018.12.001
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Akbari, Sweeta Nour, A. H. R. M., Yunus Alara, Oluwaseun Ruth Abayomi, Olalere Olusegun Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil
description	Fenugreek (Trigonella-Foenum Graecum) is known as one of the traditional and most promising medicinal herbs belongs to the leguminous family. The seeds of fenugreek have been extensively studied for the treatment of inflammation, cancer and diabetes. In this study, fenugreek seed oil was extracted and evaluated for its chemical compositions and bonding through gas chromatography coupled to mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FT-IR) analysis, respectively. The antioxidant activity against 2,2-diphenyl-picrylhydrazyl (DPPH) and 2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS+•) radicals, total phenolic content (TPC) and total flavonoid content (TFC) of the oil were also studied. The capacity of antioxidants detected by ABTS was stronger than that by DPPH. A total number of 23 chemical compounds were detected and identified in fenugreek seed oil comprising of 99% of the total oil through GC-MS analysis. The major compounds of the extracted oil were linoleic acid (54.13%), palmitic acid (16.21%), pinene (4.56%), 4-Pentyl-1-(4-propylcyclohexyl)-1-cyclohexene (3.87%) and linoleic acid methyl ester (3.19%). FTIR analysis confirmed the presence of carboxyl group in the oil which were more dominated by unsaturated essential fatty acids. Moreover, the oil of fenugreek seed indicated a strong antioxidant radical scavenging activity against both DPPH and ABTS assays with an IC50 of 172.6 ± 3.1 and 161.3 ± 2.21, respectively. The TPC and TFC of the oil were 38.97 ± 0.34 mg GAE/g. oil and 14.417 ± 0.23 mg QE/g.oil. Thus, this study suggests that the fenugreek seed oil could be used for pharmaceutical purposes.
format	Article
author	Akbari, Sweeta Nour, A. H. R. M., Yunus Alara, Oluwaseun Ruth Abayomi, Olalere Olusegun
author_facet	Akbari, Sweeta Nour, A. H. R. M., Yunus Alara, Oluwaseun Ruth Abayomi, Olalere Olusegun
author_sort	Akbari, Sweeta
title	Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil
title_short	Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil
title_full	Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil
title_fullStr	Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil
title_full_unstemmed	Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil
title_sort	extraction, characterization and antioxidant activity of fenugreek (trigonella-foenum graecum) seed oil
publisher	Elsevier Ltd
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/26644/ http://umpir.ump.edu.my/id/eprint/26644/ http://umpir.ump.edu.my/id/eprint/26644/ http://umpir.ump.edu.my/id/eprint/26644/1/Extraction%2C%20characterization%20and%20antioxidant.pdf
first_indexed	2023-09-18T22:41:36Z
last_indexed	2023-09-18T22:41:36Z
_version_	1777416951846404096

Extraction, characterization and antioxidant activity of fenugreek (Trigonella-Foenum Graecum) seed oil

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