Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples

Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples

Kefir drink is a product from the fermentation process of milk using symbiotic mixture of bacteria and yeast consortium. Saccharomyces and lactobacillus are the major genera found in the kefir drink. The present work involves the isolation and identification of potential probiotic yeast strains in t...

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Main Authors: Mohd Akmal, Azhar, Dr. Mimi Sakinah, Abdul Munaim
Format: Article
Language:English
Published: 2019
Subjects:
T Technology (General)
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/26634/
http://umpir.ump.edu.my/id/eprint/26634/
http://umpir.ump.edu.my/id/eprint/26634/
http://umpir.ump.edu.my/id/eprint/26634/1/akmalazhar2019%20%281%29.pdf
id ump-26634
recordtype eprints
spelling ump-266342019-12-05T06:23:43Z http://umpir.ump.edu.my/id/eprint/26634/ Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples Mohd Akmal, Azhar Dr. Mimi Sakinah, Abdul Munaim T Technology (General) TP Chemical technology Kefir drink is a product from the fermentation process of milk using symbiotic mixture of bacteria and yeast consortium. Saccharomyces and lactobacillus are the major genera found in the kefir drink. The present work involves the isolation and identification of potential probiotic yeast strains in the kefir drink samples from Malaysia. The molecular identification was done by PCR using ITS1 and ITS4 amplified regions. Nine different yeast strains were isolated, and the strains were successfully identified based on the sequence analysis. Saccharomyces and Kodamaea were found to be the major population in the kefir drink samples. Lastly, kefir milk is one of the excellent sources of probiotic yeast strains and could be used as a new yeast probiotic formulation or in food supplements. Moreover, the amplification of ITS region can be used as a useful method to identified yeast strains. 2019-10-04 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26634/1/akmalazhar2019%20%281%29.pdf Mohd Akmal, Azhar and Dr. Mimi Sakinah, Abdul Munaim (2019) Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples. International Journal of Pharma Medicine and Biological Sciences, 8 (4). pp. 128-131. ISSN 2278-5221 http://International Journal of Pharma Medicine and Biologica Sciences http://10.18178/ijpmbs.8.4.128-131
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic T Technology (General)
TP Chemical technology
spellingShingle T Technology (General)
TP Chemical technology
Mohd Akmal, Azhar
Dr. Mimi Sakinah, Abdul Munaim
Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples
description Kefir drink is a product from the fermentation process of milk using symbiotic mixture of bacteria and yeast consortium. Saccharomyces and lactobacillus are the major genera found in the kefir drink. The present work involves the isolation and identification of potential probiotic yeast strains in the kefir drink samples from Malaysia. The molecular identification was done by PCR using ITS1 and ITS4 amplified regions. Nine different yeast strains were isolated, and the strains were successfully identified based on the sequence analysis. Saccharomyces and Kodamaea were found to be the major population in the kefir drink samples. Lastly, kefir milk is one of the excellent sources of probiotic yeast strains and could be used as a new yeast probiotic formulation or in food supplements. Moreover, the amplification of ITS region can be used as a useful method to identified yeast strains.
format Article
author Mohd Akmal, Azhar
Dr. Mimi Sakinah, Abdul Munaim
author_facet Mohd Akmal, Azhar
Dr. Mimi Sakinah, Abdul Munaim
author_sort Mohd Akmal, Azhar
title Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples
title_short Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples
title_full Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples
title_fullStr Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples
title_full_unstemmed Isolation and Molecular Identification of Potential Probiotic Yeast Strains Found in Malaysian Kefir Drinks Samples
title_sort isolation and molecular identification of potential probiotic yeast strains found in malaysian kefir drinks samples
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/26634/
http://umpir.ump.edu.my/id/eprint/26634/
http://umpir.ump.edu.my/id/eprint/26634/
http://umpir.ump.edu.my/id/eprint/26634/1/akmalazhar2019%20%281%29.pdf
first_indexed 2023-09-18T22:41:35Z
last_indexed 2023-09-18T22:41:35Z
_version_ 1777416950425583616

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