Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater

The marine diatom Gyrosigma sp. was cultured in a medium comprised of inorganic nutrients dissolved in palm oil mill effluent (POME) wastewater. The production of lipids in the biomass was optimized using a statistical design of experiments in combination with the response surface method. The experi...

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Main Authors:	Natanamurugaraj, Govindan, Gaanty Pragas, Maniam, M. M., Yusoff, Mohd Hasbi, Ab. Rahim, Chatsungnoen, Tawan, Rameshprabu, Ramaraj, Chisti, Yusuf
Format:	Article
Language:	English
Published:	Springer Netherlands 2019
Subjects:	QD Chemistry QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/26392/ http://umpir.ump.edu.my/id/eprint/26392/ http://umpir.ump.edu.my/id/eprint/26392/ http://umpir.ump.edu.my/id/eprint/26392/1/Statistical%20optimization%20of%20lipid%20production%20by%20the%20diatom%20Gyrosigma%20sp%20grown%20in%20industrial%20wastewater.pdf

id	ump-26392
recordtype	eprints
spelling	ump-263922019-12-09T04:09:55Z http://umpir.ump.edu.my/id/eprint/26392/ Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff Mohd Hasbi, Ab. Rahim Chatsungnoen, Tawan Rameshprabu, Ramaraj Chisti, Yusuf QD Chemistry QR Microbiology The marine diatom Gyrosigma sp. was cultured in a medium comprised of inorganic nutrients dissolved in palm oil mill effluent (POME) wastewater. The production of lipids in the biomass was optimized using a statistical design of experiments in combination with the response surface method. The experimental factors were incident light level and initial concentrations of nitrate, phosphate, and silicate in the medium in batch culture. At 25 ± 2 °C, the maximum lipid content in the biomass harvested at the end of a 12-day batch culture was 70.7 ± 6.0% by dry weight for the following values of the experimental factors: an incident light level of 131 μmol photons m−2 s−1, a nitrate concentration of 1.8 mg L−1 (29.0 μM), a phosphate concentration of 6.8 mg L−1 (71.6 μM), and a silicate concentration of 10.1 mg L−1 (132.7 μM). Under the optimized conditions, the maximum dry mass concentration of the diatom was 560 mg L−1 on day 8 of a batch culture, declining to ~409 mg L−1 on day 12. For the 12-day batch operation, the final average productivities of the biomass and the lipids were 34.1 ± 5.5 mg L−1 day−1 and 24.1 ± 0.2 mg L−1 day−1, respectively. The fatty acids in the algal lipids were found to be as follows (%, w/w of total lipids): palmitic acid (48.6%), eicosapentaenoic acid (10.6%), myristic acid (8.1%), stearic acid (8.0%), linoleic acid (7.5%), oleic acid (6.4%), and linolenic acid (5.8%). The response surface model predicted the lipid content in the biomass with a high degree of confidence. Springer Netherlands 2019-11-07 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26392/1/Statistical%20optimization%20of%20lipid%20production%20by%20the%20diatom%20Gyrosigma%20sp%20grown%20in%20industrial%20wastewater.pdf Natanamurugaraj, Govindan and Gaanty Pragas, Maniam and M. M., Yusoff and Mohd Hasbi, Ab. Rahim and Chatsungnoen, Tawan and Rameshprabu, Ramaraj and Chisti, Yusuf (2019) Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater. Journal of Applied Phycology. ISSN 1573-5176 (In Press) https://doi.org/10.1007/s10811-019-01971-x https://doi.org/10.1007/s10811-019-01971-x
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry QR Microbiology
spellingShingle	QD Chemistry QR Microbiology Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff Mohd Hasbi, Ab. Rahim Chatsungnoen, Tawan Rameshprabu, Ramaraj Chisti, Yusuf Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater
description	The marine diatom Gyrosigma sp. was cultured in a medium comprised of inorganic nutrients dissolved in palm oil mill effluent (POME) wastewater. The production of lipids in the biomass was optimized using a statistical design of experiments in combination with the response surface method. The experimental factors were incident light level and initial concentrations of nitrate, phosphate, and silicate in the medium in batch culture. At 25 ± 2 °C, the maximum lipid content in the biomass harvested at the end of a 12-day batch culture was 70.7 ± 6.0% by dry weight for the following values of the experimental factors: an incident light level of 131 μmol photons m−2 s−1, a nitrate concentration of 1.8 mg L−1 (29.0 μM), a phosphate concentration of 6.8 mg L−1 (71.6 μM), and a silicate concentration of 10.1 mg L−1 (132.7 μM). Under the optimized conditions, the maximum dry mass concentration of the diatom was 560 mg L−1 on day 8 of a batch culture, declining to ~409 mg L−1 on day 12. For the 12-day batch operation, the final average productivities of the biomass and the lipids were 34.1 ± 5.5 mg L−1 day−1 and 24.1 ± 0.2 mg L−1 day−1, respectively. The fatty acids in the algal lipids were found to be as follows (%, w/w of total lipids): palmitic acid (48.6%), eicosapentaenoic acid (10.6%), myristic acid (8.1%), stearic acid (8.0%), linoleic acid (7.5%), oleic acid (6.4%), and linolenic acid (5.8%). The response surface model predicted the lipid content in the biomass with a high degree of confidence.
format	Article
author	Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff Mohd Hasbi, Ab. Rahim Chatsungnoen, Tawan Rameshprabu, Ramaraj Chisti, Yusuf
author_facet	Natanamurugaraj, Govindan Gaanty Pragas, Maniam M. M., Yusoff Mohd Hasbi, Ab. Rahim Chatsungnoen, Tawan Rameshprabu, Ramaraj Chisti, Yusuf
author_sort	Natanamurugaraj, Govindan
title	Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater
title_short	Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater
title_full	Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater
title_fullStr	Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater
title_full_unstemmed	Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater
title_sort	statistical optimization of lipid production by the diatom gyrosigma sp. grown in industrial wastewater
publisher	Springer Netherlands
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/26392/ http://umpir.ump.edu.my/id/eprint/26392/ http://umpir.ump.edu.my/id/eprint/26392/ http://umpir.ump.edu.my/id/eprint/26392/1/Statistical%20optimization%20of%20lipid%20production%20by%20the%20diatom%20Gyrosigma%20sp%20grown%20in%20industrial%20wastewater.pdf
first_indexed	2023-09-18T22:41:04Z
last_indexed	2023-09-18T22:41:04Z
_version_	1777416918413606912

Statistical optimization of lipid production by the diatom Gyrosigma sp. grown in industrial wastewater

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