Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice

The yield of Ananas comosus (pineapple) is susceptible to microbial infection such as fusariosis which normally affecting the fruits and the leaves. Usage of chemical pesticides to control these diseases has been often associated with negative impacts on the environment and human health. Since pinea...

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Main Authors:	Putri Nurul Yasmin, Mohd Ridza, Siti Hatijah, Mortan
Format:	Conference or Workshop Item
Language:	English English
Published:	Universiti Malaysia Pahang 2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/26367/ http://umpir.ump.edu.my/id/eprint/26367/1/24.%20Application%20of%20two%20level%20factorial%20design%20to%20study.pdf http://umpir.ump.edu.my/id/eprint/26367/2/24.1%20Application%20of%20two%20level%20factorial%20design%20to%20study.pdf

id	ump-26367
recordtype	eprints
spelling	ump-263672019-12-23T02:50:17Z http://umpir.ump.edu.my/id/eprint/26367/ Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice Putri Nurul Yasmin, Mohd Ridza Siti Hatijah, Mortan TP Chemical technology The yield of Ananas comosus (pineapple) is susceptible to microbial infection such as fusariosis which normally affecting the fruits and the leaves. Usage of chemical pesticides to control these diseases has been often associated with negative impacts on the environment and human health. Since pineapple leaves are one of the lavishly organic waste materials which contains total phenolic content (TPC) with antimicrobial properties, it was desired to gain deeper knowledge on the potential of pineapple leaves as microbial growth inhibitor. The objective of this research is to study the factors that affect microbe growth inhibition using pineapple leaves juice (PLJ). The factors evaluated were; reaction time between mixtures of PLJ with microbe (0.5 – 5 hours), concentration of TPC in PLJ (0.2563 – 0.5127 mg GAE/mL), reaction temperature (26 – 37°C), and ratio of microbe to PLJ (M/PLJ) (1:1 and 1:3). A two level factorial design was adopted to assess the effect of the above mentioned factors on the microbial inhibition by PLJ. The results showed that the most contributing factor of 1.55% was reaction temperature, meanwhile the highest contribution factor for interaction effect was between reaction time between mixtures of PLJ with microbe and M/PLJ at 4.26%. The best condition for microbe growth inhibition of 20.90% was found to be at reaction time of 0.5 hour, TPC in PLJ of 0.5127 mg GAE/mL, reaction temperature of 37°C, and M/PLJ at 1:1. Further study to optimize the conditions of the PLJ to achieve higher percentage of microbial inhibition is therefore inevitable. This study demonstrates that pineapple leaves could be exploited as valuable sources of natural products that could be used as microbial growth inhibitor and thus become one of the cheap and green alternatives for more expensive chemical pesticides. Universiti Malaysia Pahang 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26367/1/24.%20Application%20of%20two%20level%20factorial%20design%20to%20study.pdf pdf en http://umpir.ump.edu.my/id/eprint/26367/2/24.1%20Application%20of%20two%20level%20factorial%20design%20to%20study.pdf Putri Nurul Yasmin, Mohd Ridza and Siti Hatijah, Mortan (2019) Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice. In: Energy Security And Chemical Engineering Congress 2019 (ESCHE 2019), 17-19 July 2019 , Parkroyal Resort, Penang, Malaysia. pp. 1-11.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Putri Nurul Yasmin, Mohd Ridza Siti Hatijah, Mortan Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
description	The yield of Ananas comosus (pineapple) is susceptible to microbial infection such as fusariosis which normally affecting the fruits and the leaves. Usage of chemical pesticides to control these diseases has been often associated with negative impacts on the environment and human health. Since pineapple leaves are one of the lavishly organic waste materials which contains total phenolic content (TPC) with antimicrobial properties, it was desired to gain deeper knowledge on the potential of pineapple leaves as microbial growth inhibitor. The objective of this research is to study the factors that affect microbe growth inhibition using pineapple leaves juice (PLJ). The factors evaluated were; reaction time between mixtures of PLJ with microbe (0.5 – 5 hours), concentration of TPC in PLJ (0.2563 – 0.5127 mg GAE/mL), reaction temperature (26 – 37°C), and ratio of microbe to PLJ (M/PLJ) (1:1 and 1:3). A two level factorial design was adopted to assess the effect of the above mentioned factors on the microbial inhibition by PLJ. The results showed that the most contributing factor of 1.55% was reaction temperature, meanwhile the highest contribution factor for interaction effect was between reaction time between mixtures of PLJ with microbe and M/PLJ at 4.26%. The best condition for microbe growth inhibition of 20.90% was found to be at reaction time of 0.5 hour, TPC in PLJ of 0.5127 mg GAE/mL, reaction temperature of 37°C, and M/PLJ at 1:1. Further study to optimize the conditions of the PLJ to achieve higher percentage of microbial inhibition is therefore inevitable. This study demonstrates that pineapple leaves could be exploited as valuable sources of natural products that could be used as microbial growth inhibitor and thus become one of the cheap and green alternatives for more expensive chemical pesticides.
format	Conference or Workshop Item
author	Putri Nurul Yasmin, Mohd Ridza Siti Hatijah, Mortan
author_facet	Putri Nurul Yasmin, Mohd Ridza Siti Hatijah, Mortan
author_sort	Putri Nurul Yasmin, Mohd Ridza
title	Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
title_short	Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
title_full	Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
title_fullStr	Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
title_full_unstemmed	Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
title_sort	application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice
publisher	Universiti Malaysia Pahang
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/26367/ http://umpir.ump.edu.my/id/eprint/26367/1/24.%20Application%20of%20two%20level%20factorial%20design%20to%20study.pdf http://umpir.ump.edu.my/id/eprint/26367/2/24.1%20Application%20of%20two%20level%20factorial%20design%20to%20study.pdf
first_indexed	2023-09-18T22:41:01Z
last_indexed	2023-09-18T22:41:01Z
_version_	1777416914784485376

Application of two level factorial design to study the microbe growth inhibition by pineapple leaves juice

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