Microbial cell wall distruption by electroporation

Electroporation technique was conducted in these project with an objective to disrupt the microbial cell wall. In these experiment, two factor are to be consider to know the best condition for electroporation method. Firstly, the effect of distance between two electrode and the effect of treatment t...

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Main Author:	Muhammad Faris Hanif, Yusoff
Format:	Undergraduates Project Papers
Language:	English
Published:	2016
Subjects:	T Technology (General) TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/25654/ http://umpir.ump.edu.my/id/eprint/25654/ http://umpir.ump.edu.my/id/eprint/25654/1/Microbial%20cell%20wall%20distruption.pdf

id	ump-25654
recordtype	eprints
spelling	ump-256542019-08-16T07:45:55Z http://umpir.ump.edu.my/id/eprint/25654/ Microbial cell wall distruption by electroporation Muhammad Faris Hanif, Yusoff T Technology (General) TA Engineering (General). Civil engineering (General) Electroporation technique was conducted in these project with an objective to disrupt the microbial cell wall. In these experiment, two factor are to be consider to know the best condition for electroporation method. Firstly, the effect of distance between two electrode and the effect of treatment time towards the sample of yeast. The objective can be justified by two method, Colony Forming unit (CFU) and FESEM analysis. In CFU method, two experiment with 4 test was conducted. First experiment was 2 cm electrode distance with the treatment time for test 1 was 4 minutes and test 2 of 10 minutes. The second experiment, 4 cm electrode distance with treatment time for test 1 was 4 minutes and test 2 of 10 minutes. Based on the result, we can conclude that the trend show as the treatment time increase, the CFU count decreases. FESEM analysis show the breakup of the cell wall. Two image of untreated yeast cell and treated yeast cell with electroporation was compared ell. Treated yeast cell show the breakup of the cell wall. The optimum condition of electroporation was then being analyzed. From the result, we can conclude that the optimum condition for electroporation technique were determined to be 2 cm electrode distance and 4 minutes of treatment time due to some factor. As a conclusion, the objective of these experiment is achieved by the breaking of the cell wall and extraction of lipid content. 2016-01 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25654/1/Microbial%20cell%20wall%20distruption.pdf Muhammad Faris Hanif, Yusoff (2016) Microbial cell wall distruption by electroporation. Faculty of Engineering Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:101079&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	T Technology (General) TA Engineering (General). Civil engineering (General)
spellingShingle	T Technology (General) TA Engineering (General). Civil engineering (General) Muhammad Faris Hanif, Yusoff Microbial cell wall distruption by electroporation
description	Electroporation technique was conducted in these project with an objective to disrupt the microbial cell wall. In these experiment, two factor are to be consider to know the best condition for electroporation method. Firstly, the effect of distance between two electrode and the effect of treatment time towards the sample of yeast. The objective can be justified by two method, Colony Forming unit (CFU) and FESEM analysis. In CFU method, two experiment with 4 test was conducted. First experiment was 2 cm electrode distance with the treatment time for test 1 was 4 minutes and test 2 of 10 minutes. The second experiment, 4 cm electrode distance with treatment time for test 1 was 4 minutes and test 2 of 10 minutes. Based on the result, we can conclude that the trend show as the treatment time increase, the CFU count decreases. FESEM analysis show the breakup of the cell wall. Two image of untreated yeast cell and treated yeast cell with electroporation was compared ell. Treated yeast cell show the breakup of the cell wall. The optimum condition of electroporation was then being analyzed. From the result, we can conclude that the optimum condition for electroporation technique were determined to be 2 cm electrode distance and 4 minutes of treatment time due to some factor. As a conclusion, the objective of these experiment is achieved by the breaking of the cell wall and extraction of lipid content.
format	Undergraduates Project Papers
author	Muhammad Faris Hanif, Yusoff
author_facet	Muhammad Faris Hanif, Yusoff
author_sort	Muhammad Faris Hanif, Yusoff
title	Microbial cell wall distruption by electroporation
title_short	Microbial cell wall distruption by electroporation
title_full	Microbial cell wall distruption by electroporation
title_fullStr	Microbial cell wall distruption by electroporation
title_full_unstemmed	Microbial cell wall distruption by electroporation
title_sort	microbial cell wall distruption by electroporation
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/25654/ http://umpir.ump.edu.my/id/eprint/25654/ http://umpir.ump.edu.my/id/eprint/25654/1/Microbial%20cell%20wall%20distruption.pdf
first_indexed	2023-09-18T22:39:31Z
last_indexed	2023-09-18T22:39:31Z
_version_	1777416820637040640

Microbial cell wall distruption by electroporation

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