Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis

The prevalence of extended spectrum β-lactamases (ESBLs), metalloβ-lactamases (MBL), and plasmid-mediated resistance is a severe threat to current lactam therapy leading to treatment failure. Therefore, in this research, an investigation has been conducted to study the presence of ESBL and MBL genes...

Full description

Bibliographic Details
Main Authors:	M. K. K., Fazlul, Rashid, Shah Samiur, Aizi Nor Mazila, Ramli, Ukaegbu, Chinonso Ishmael, M. H. M., Nazmul
Format:	Article
Language:	English English
Published:	Hashemite University 2019
Subjects:	QD Chemistry T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/25548/ http://umpir.ump.edu.my/id/eprint/25548/1/JJBS%20Fazlul%20Paper%20number%2017%20%289%29%20%282%29.doc http://umpir.ump.edu.my/id/eprint/25548/2/jordan%20Journal%20of%20Biological%20science.pdf

id	ump-25548
recordtype	eprints
spelling	ump-255482019-11-13T08:20:51Z http://umpir.ump.edu.my/id/eprint/25548/ Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis M. K. K., Fazlul Rashid, Shah Samiur Aizi Nor Mazila, Ramli Ukaegbu, Chinonso Ishmael M. H. M., Nazmul QD Chemistry T Technology (General) The prevalence of extended spectrum β-lactamases (ESBLs), metalloβ-lactamases (MBL), and plasmid-mediated resistance is a severe threat to current lactam therapy leading to treatment failure. Therefore, in this research, an investigation has been conducted to study the presence of ESBL and MBL genes in Acinetobacter spp. and their relationship to antimicrobial resistance. Fifty Acinetobacter spp. isolates were collected from both pediatric and adult patients at Sungai Buloh Hospital, Kuala Lumpur, Malaysia. The Double Disk Synergy Test (DDST) and the E-test have been used to detect ESBL and MBL production. The plasmid was extracted using the Close and Rodriguez (1982) method with modification in addition to the QIAprep Spin Kit. Among the fifty Acinetobacter isolates, eleven (22%) were ESBL-positive, while none of the strains produced MBLs. Fourteen isolates were positive for a plasmid with an occurrence rate (POR) of 28 %. These fourteen isolates appeared to harbor one or more plasmids with a maximum of four plasmids. Four isolates had single plasmids, and five carried four plasmids, while four and one isolates had two and one plasmids respectively. The sizes of the plasmid DNA were found to range between 1.8 kb and 14 kb. This study emphasizes the problematic raising frequency of ESBL, MBL, and plasmid-mediated resistance. This awareness highlights an urgent need to prevent and control such bacteria Hashemite University 2019 Article PeerReviewed text en http://umpir.ump.edu.my/id/eprint/25548/1/JJBS%20Fazlul%20Paper%20number%2017%20%289%29%20%282%29.doc pdf en http://umpir.ump.edu.my/id/eprint/25548/2/jordan%20Journal%20of%20Biological%20science.pdf M. K. K., Fazlul and Rashid, Shah Samiur and Aizi Nor Mazila, Ramli and Ukaegbu, Chinonso Ishmael and M. H. M., Nazmul (2019) Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis. Jordan Journal of Biological Sciences, 12 (3). pp. 373-378. ISSN 2307-7166
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	QD Chemistry T Technology (General)
spellingShingle	QD Chemistry T Technology (General) M. K. K., Fazlul Rashid, Shah Samiur Aizi Nor Mazila, Ramli Ukaegbu, Chinonso Ishmael M. H. M., Nazmul Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis
description	The prevalence of extended spectrum β-lactamases (ESBLs), metalloβ-lactamases (MBL), and plasmid-mediated resistance is a severe threat to current lactam therapy leading to treatment failure. Therefore, in this research, an investigation has been conducted to study the presence of ESBL and MBL genes in Acinetobacter spp. and their relationship to antimicrobial resistance. Fifty Acinetobacter spp. isolates were collected from both pediatric and adult patients at Sungai Buloh Hospital, Kuala Lumpur, Malaysia. The Double Disk Synergy Test (DDST) and the E-test have been used to detect ESBL and MBL production. The plasmid was extracted using the Close and Rodriguez (1982) method with modification in addition to the QIAprep Spin Kit. Among the fifty Acinetobacter isolates, eleven (22%) were ESBL-positive, while none of the strains produced MBLs. Fourteen isolates were positive for a plasmid with an occurrence rate (POR) of 28 %. These fourteen isolates appeared to harbor one or more plasmids with a maximum of four plasmids. Four isolates had single plasmids, and five carried four plasmids, while four and one isolates had two and one plasmids respectively. The sizes of the plasmid DNA were found to range between 1.8 kb and 14 kb. This study emphasizes the problematic raising frequency of ESBL, MBL, and plasmid-mediated resistance. This awareness highlights an urgent need to prevent and control such bacteria
format	Article
author	M. K. K., Fazlul Rashid, Shah Samiur Aizi Nor Mazila, Ramli Ukaegbu, Chinonso Ishmael M. H. M., Nazmul
author_facet	M. K. K., Fazlul Rashid, Shah Samiur Aizi Nor Mazila, Ramli Ukaegbu, Chinonso Ishmael M. H. M., Nazmul
author_sort	M. K. K., Fazlul
title	Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis
title_short	Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis
title_full	Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis
title_fullStr	Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis
title_full_unstemmed	Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis
title_sort	detection of esbl and mbl in acinetobacter spp. and their plasmid profile analysis
publisher	Hashemite University
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/25548/ http://umpir.ump.edu.my/id/eprint/25548/1/JJBS%20Fazlul%20Paper%20number%2017%20%289%29%20%282%29.doc http://umpir.ump.edu.my/id/eprint/25548/2/jordan%20Journal%20of%20Biological%20science.pdf
first_indexed	2023-09-18T22:39:17Z
last_indexed	2023-09-18T22:39:17Z
_version_	1777416806001016832

Detection of ESBL and MBL in Acinetobacter spp. and their plasmid profile analysis

Similar Items