Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-cente...

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Main Authors:	Zatul Iffah, Mohd Arshad, Azura, Amid
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf

id	ump-25502
recordtype	eprints
spelling	ump-255022019-11-21T03:24:52Z http://umpir.ump.edu.my/id/eprint/25502/ Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology Zatul Iffah, Mohd Arshad Azura, Amid TP Chemical technology Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf pdf en http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf Zatul Iffah, Mohd Arshad and Azura, Amid (2018) Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology. In: The 4th International Conference On The Applications Of Science And Mathematics 2018, 13 - 14 Ogos 2018 , Tunku Tun Aminah Library UTHM, Parit Raja, Johor. pp. 377-382., 7 (4.30). http://dx.doi.org/10.14419/ijet.v7i4.30.22326
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Zatul Iffah, Mohd Arshad Azura, Amid Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
description	Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.
format	Conference or Workshop Item
author	Zatul Iffah, Mohd Arshad Azura, Amid
author_facet	Zatul Iffah, Mohd Arshad Azura, Amid
author_sort	Zatul Iffah, Mohd Arshad
title	Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_short	Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_full	Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_fullStr	Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_full_unstemmed	Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_sort	optimization of aqueous two-phase system (atps) of recombinant bromelain by response surface methodology
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf
first_indexed	2023-09-18T22:39:11Z
last_indexed	2023-09-18T22:39:11Z
_version_	1777416800089145344

Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology

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