Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-cente...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf |
| Summary: | Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel. |
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