Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane

Membrane chromatography can overcome some of the limitations of packed bed column chromatography but preparation of adsorptive membranes usually involves complex and harsh chemical modifications. Mixed matrix membranes (MMMs) require only the physical incorporation of an ion exchange resin into the...

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Main Authors:	Syed Mohd Saufi, Tuan Chik, J. Fee, Conan
Format:	Article
Language:	English
Published:	Elsevier B.V. 2011
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25437/ http://umpir.ump.edu.my/id/eprint/25437/ http://umpir.ump.edu.my/id/eprint/25437/ http://umpir.ump.edu.my/id/eprint/25437/1/Simultaneous%20anion%20and%20cation%20exchange%20chromatography%20of%20whey%20proteins.pdf

id	ump-25437
recordtype	eprints
spelling	ump-254372019-12-24T02:34:19Z http://umpir.ump.edu.my/id/eprint/25437/ Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane Syed Mohd Saufi, Tuan Chik J. Fee, Conan TP Chemical technology Membrane chromatography can overcome some of the limitations of packed bed column chromatography but preparation of adsorptive membranes usually involves complex and harsh chemical modifications. Mixed matrix membranes (MMMs) require only the physical incorporation of an ion exchange resin into the membrane polymer solution prior to membrane casting. An advantage of MMMs not previously exploited is that resins with differing adsorptive functionalities can be conveniently embedded within a single membrane at any desired ratio. This presents the opportunity to customize an adsorptive membrane to suit the expected protein profile of a raw feed stream e.g. bovine whey or serum. In this work, a novel mixed mode interaction MMM customized to extract all major proteins from bovine whey was synthesized in a single membrane by incorporating 42.5 wt% Lewatit MP500 anionic resin and 7.5 wt% SP Sepharose cationic resin into an ethylene vinyl alcohol base polymer casting solution. The mixed mode MMM developed was able to bind both basic and acidic proteins simultaneously from whey, with binding capacities of 7.16 ± 2.24 mg α-lactalbumin g−1 membrane, 11.40 ± 0.73 mg lactoferrin (LF) g−1 membrane, 59.21 ± 9.90 mg β-lactoglobulin g−1 membrane and 6.79 ± 1.11 mg immunoglobulin G g−1 membrane (85 mg total protein g−1 membrane) during batch fractionation of LF-spiked whey. A 1000 m2 spiral-wound membrane module (200 L membrane volume, 1 m3 module volume) is predicted to be able to produce approximately 25 kg total whey protein per h. Elsevier B.V. 2011 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25437/1/Simultaneous%20anion%20and%20cation%20exchange%20chromatography%20of%20whey%20proteins.pdf Syed Mohd Saufi, Tuan Chik and J. Fee, Conan (2011) Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane. Journal of Chromatography A, 1218 (50). pp. 9003-9009. ISSN 0021-9673 https://doi.org/10.1016/j.chroma.2011.09.045 https://doi.org/10.1016/j.chroma.2011.09.045
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Syed Mohd Saufi, Tuan Chik J. Fee, Conan Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
description	Membrane chromatography can overcome some of the limitations of packed bed column chromatography but preparation of adsorptive membranes usually involves complex and harsh chemical modifications. Mixed matrix membranes (MMMs) require only the physical incorporation of an ion exchange resin into the membrane polymer solution prior to membrane casting. An advantage of MMMs not previously exploited is that resins with differing adsorptive functionalities can be conveniently embedded within a single membrane at any desired ratio. This presents the opportunity to customize an adsorptive membrane to suit the expected protein profile of a raw feed stream e.g. bovine whey or serum. In this work, a novel mixed mode interaction MMM customized to extract all major proteins from bovine whey was synthesized in a single membrane by incorporating 42.5 wt% Lewatit MP500 anionic resin and 7.5 wt% SP Sepharose cationic resin into an ethylene vinyl alcohol base polymer casting solution. The mixed mode MMM developed was able to bind both basic and acidic proteins simultaneously from whey, with binding capacities of 7.16 ± 2.24 mg α-lactalbumin g−1 membrane, 11.40 ± 0.73 mg lactoferrin (LF) g−1 membrane, 59.21 ± 9.90 mg β-lactoglobulin g−1 membrane and 6.79 ± 1.11 mg immunoglobulin G g−1 membrane (85 mg total protein g−1 membrane) during batch fractionation of LF-spiked whey. A 1000 m2 spiral-wound membrane module (200 L membrane volume, 1 m3 module volume) is predicted to be able to produce approximately 25 kg total whey protein per h.
format	Article
author	Syed Mohd Saufi, Tuan Chik J. Fee, Conan
author_facet	Syed Mohd Saufi, Tuan Chik J. Fee, Conan
author_sort	Syed Mohd Saufi, Tuan Chik
title	Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
title_short	Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
title_full	Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
title_fullStr	Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
title_full_unstemmed	Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
title_sort	simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane
publisher	Elsevier B.V.
publishDate	2011
url	http://umpir.ump.edu.my/id/eprint/25437/ http://umpir.ump.edu.my/id/eprint/25437/ http://umpir.ump.edu.my/id/eprint/25437/ http://umpir.ump.edu.my/id/eprint/25437/1/Simultaneous%20anion%20and%20cation%20exchange%20chromatography%20of%20whey%20proteins.pdf
first_indexed	2023-09-18T22:39:03Z
last_indexed	2023-09-18T22:39:03Z
_version_	1777416791906058240

Simultaneous anion and cation exchange chromatography of whey proteins using a customizable mixed matrix membrane

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