Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts

The medicinal plants were good source of phytochemicals and active compounds. In order to find the most potential plant extracts for the mixture purpose that could give combination effect for antimicrobial and antioxidant activities. The targeted plants are A. bilimbi, O. stamineus, E. longifolia, P...

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Main Authors:	Mahmood, Mahmood H., Osama, A K, Makky, Essam A., Mohd Hasbi, Ab. Rahim, Nor Hafizah, Mat Ali, Nur Diana, Hazrudin
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	QA Mathematics T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/25391/ http://umpir.ump.edu.my/id/eprint/25391/1/6.%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf http://umpir.ump.edu.my/id/eprint/25391/2/6.1%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf

id	ump-25391
recordtype	eprints
spelling	ump-253912020-02-28T01:26:24Z http://umpir.ump.edu.my/id/eprint/25391/ Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts Mahmood, Mahmood H. Osama, A K Makky, Essam A. Mohd Hasbi, Ab. Rahim Nor Hafizah, Mat Ali Nur Diana, Hazrudin QA Mathematics T Technology (General) The medicinal plants were good source of phytochemicals and active compounds. In order to find the most potential plant extracts for the mixture purpose that could give combination effect for antimicrobial and antioxidant activities. The targeted plants are A. bilimbi, O. stamineus, E. longifolia, P. granatum, and M. nigra. Ultrasound extraction technique was used for extraction. The yield of plant extract was test for detecting phytochemical content. After the detection of compound, the plant extracts were screened at 3 different concentrations (600, 400 and 200 mg/mL) against five microorganisms (S. aureus, B. subtilis, P. vulgaris, E. coli, and C. albicans) using disc diffusion assay method for selecting the potential extract. The result showed that P. ganatum and M. nigra have highest antimicrobial activity against all the test organisms and selected for extract mixture and screened for antimicrobial activity at the same above mentioned concentrations. The DPPH scavenging activity of the individual plant extracts, P. granatum and O. stamineus gave the highest activities of 95.40 and 90.20%, respectively. Also gave the lowest IC50 of 120.2 and 330.0 μg/mL, respectively, which indicate good antioxidant agents. Both extracts were selected for antioxidant combined action effect at ratio of P. granatum: O. stamineus (v/v) 1:1, 1:2 and 2:1 and all of the mixtures exhibited good scavenging activities of 94.31, 95.40 and 90.20%, respectively. However, the mixture with ratio 1:1 was the best antioxidant due to the IC50 of 82.0 μg/mL obtained. It was concluded that the mixture of chosen extracts can be used as strong antioxidant properties as well as antimicrobial activity and the extract mixture reported as successful antifungal agent. 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25391/1/6.%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf pdf en http://umpir.ump.edu.my/id/eprint/25391/2/6.1%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf Mahmood, Mahmood H. and Osama, A K and Makky, Essam A. and Mohd Hasbi, Ab. Rahim and Nor Hafizah, Mat Ali and Nur Diana, Hazrudin (2019) Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts. In: The 2nd international conference on applied biochemistry and biotechnology (ABB 2019), 21 - 24 Julai 2019 , Macau, China. pp. 1-17.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	QA Mathematics T Technology (General)
spellingShingle	QA Mathematics T Technology (General) Mahmood, Mahmood H. Osama, A K Makky, Essam A. Mohd Hasbi, Ab. Rahim Nor Hafizah, Mat Ali Nur Diana, Hazrudin Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
description	The medicinal plants were good source of phytochemicals and active compounds. In order to find the most potential plant extracts for the mixture purpose that could give combination effect for antimicrobial and antioxidant activities. The targeted plants are A. bilimbi, O. stamineus, E. longifolia, P. granatum, and M. nigra. Ultrasound extraction technique was used for extraction. The yield of plant extract was test for detecting phytochemical content. After the detection of compound, the plant extracts were screened at 3 different concentrations (600, 400 and 200 mg/mL) against five microorganisms (S. aureus, B. subtilis, P. vulgaris, E. coli, and C. albicans) using disc diffusion assay method for selecting the potential extract. The result showed that P. ganatum and M. nigra have highest antimicrobial activity against all the test organisms and selected for extract mixture and screened for antimicrobial activity at the same above mentioned concentrations. The DPPH scavenging activity of the individual plant extracts, P. granatum and O. stamineus gave the highest activities of 95.40 and 90.20%, respectively. Also gave the lowest IC50 of 120.2 and 330.0 μg/mL, respectively, which indicate good antioxidant agents. Both extracts were selected for antioxidant combined action effect at ratio of P. granatum: O. stamineus (v/v) 1:1, 1:2 and 2:1 and all of the mixtures exhibited good scavenging activities of 94.31, 95.40 and 90.20%, respectively. However, the mixture with ratio 1:1 was the best antioxidant due to the IC50 of 82.0 μg/mL obtained. It was concluded that the mixture of chosen extracts can be used as strong antioxidant properties as well as antimicrobial activity and the extract mixture reported as successful antifungal agent.
format	Conference or Workshop Item
author	Mahmood, Mahmood H. Osama, A K Makky, Essam A. Mohd Hasbi, Ab. Rahim Nor Hafizah, Mat Ali Nur Diana, Hazrudin
author_facet	Mahmood, Mahmood H. Osama, A K Makky, Essam A. Mohd Hasbi, Ab. Rahim Nor Hafizah, Mat Ali Nur Diana, Hazrudin
author_sort	Mahmood, Mahmood H.
title	Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_short	Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_full	Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_fullStr	Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_full_unstemmed	Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_sort	phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/25391/ http://umpir.ump.edu.my/id/eprint/25391/1/6.%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf http://umpir.ump.edu.my/id/eprint/25391/2/6.1%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf
first_indexed	2023-09-18T22:38:58Z
last_indexed	2023-09-18T22:38:58Z
_version_	1777416785861017600

Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts

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