Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)

Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)

Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator  C C U G 5 2 2 3 8T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were in...

Full description

Bibliographic Details
Main Authors: Mior Ahmad Khushairi, Mohd Zahari, Hidayah, Ariffin, Mohd Noriznan, Mokhtar, Jailani, Salihon, Shirai, Yoshihito, Mohd Ali, Hassan
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/25286/
http://umpir.ump.edu.my/id/eprint/25286/
http://umpir.ump.edu.my/id/eprint/25286/
http://umpir.ump.edu.my/id/eprint/25286/1/Factors%20affecting%20poly%283-hydroxybutyrate%29%20production%20from%20oil%20palm.pdf
id ump-25286
recordtype eprints
spelling ump-252862019-07-18T02:22:55Z http://umpir.ump.edu.my/id/eprint/25286/ Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) Mior Ahmad Khushairi, Mohd Zahari Hidayah, Ariffin Mohd Noriznan, Mokhtar Jailani, Salihon Shirai, Yoshihito Mohd Ali, Hassan TP Chemical technology Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator  C C U G 5 2 2 3 8T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ±0.13 g/L and 45 ±1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate. Hindawi Publishing Corporation 2012 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25286/1/Factors%20affecting%20poly%283-hydroxybutyrate%29%20production%20from%20oil%20palm.pdf Mior Ahmad Khushairi, Mohd Zahari and Hidayah, Ariffin and Mohd Noriznan, Mokhtar and Jailani, Salihon and Shirai, Yoshihito and Mohd Ali, Hassan (2012) Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T). Journal of Biomedicine and Biotechnology, 2012 (125865). pp. 1-8. ISSN 1110-7243 https://doi.org/10.1155/2012/125865 https://doi.org/10.1155/2012/125865
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mior Ahmad Khushairi, Mohd Zahari
Hidayah, Ariffin
Mohd Noriznan, Mokhtar
Jailani, Salihon
Shirai, Yoshihito
Mohd Ali, Hassan
Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
description Factors influencing poly(3-hydroxybutyrate) P(3HB) production by  Cupriavidus necator  C C U G 5 2 2 3 8T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ±0.13 g/L and 45 ±1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate.
format Article
author Mior Ahmad Khushairi, Mohd Zahari
Hidayah, Ariffin
Mohd Noriznan, Mokhtar
Jailani, Salihon
Shirai, Yoshihito
Mohd Ali, Hassan
author_facet Mior Ahmad Khushairi, Mohd Zahari
Hidayah, Ariffin
Mohd Noriznan, Mokhtar
Jailani, Salihon
Shirai, Yoshihito
Mohd Ali, Hassan
author_sort Mior Ahmad Khushairi, Mohd Zahari
title Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
title_short Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
title_full Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
title_fullStr Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
title_full_unstemmed Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
title_sort factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (ccug52238 t)
publisher Hindawi Publishing Corporation
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/25286/
http://umpir.ump.edu.my/id/eprint/25286/
http://umpir.ump.edu.my/id/eprint/25286/
http://umpir.ump.edu.my/id/eprint/25286/1/Factors%20affecting%20poly%283-hydroxybutyrate%29%20production%20from%20oil%20palm.pdf
first_indexed 2023-09-18T22:38:45Z
last_indexed 2023-09-18T22:38:45Z
_version_ 1777416772345921536

Similar Items