GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties

Bioceramic nanoparticles with high specific surface area often tend to agglomerate in the polymer matrix, which results in undesirable mechanical properties of the composites and poor cell spreading and attachment. In the present work, bredigite (BR) nanoparticles were modified with an organosilane...

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Main Authors:	Kouhi, Monireh, Jayarama Reddy, Venugopal, Ramakrishna, Seeram
Format:	Article
Language:	English
Published:	Springer 2019
Subjects:	QD Chemistry R Medicine (General) RD Surgery TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25208/ http://umpir.ump.edu.my/id/eprint/25208/ http://umpir.ump.edu.my/id/eprint/25208/ http://umpir.ump.edu.my/id/eprint/25208/1/GPTMS-modified%20bredigite-PHBV%20nanofibrous%20bone%20scaffolds%20.pdf

id	ump-25208
recordtype	eprints
spelling	ump-252082019-07-03T08:34:00Z http://umpir.ump.edu.my/id/eprint/25208/ GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties Kouhi, Monireh Jayarama Reddy, Venugopal Ramakrishna, Seeram QD Chemistry R Medicine (General) RD Surgery TP Chemical technology Bioceramic nanoparticles with high specific surface area often tend to agglomerate in the polymer matrix, which results in undesirable mechanical properties of the composites and poor cell spreading and attachment. In the present work, bredigite (BR) nanoparticles were modified with an organosilane coupling agent, 3-glycidoxypropyltrimethoxysilane (GPTMS), to enhance its dispersibility in the polymer matrix. The polyhydroxybutyrate-co-hydroxyvaletare (PHBV) nanofibrous scaffolds containing either bredigite or GPTMS-modified bredigite (G-BR) nanoparticles were fabricated using electrospinning technique and characterized using scanning electron microscopy, transmission electron microscopy, and tensile strength. Results demonstrated that modification of bredigite was effective in enhancing nanoparticle dispersion in the PHBV matrix. PHBV/G-BR scaffold showed improved mechanical properties compared to PHBV and PHBV/BR, especially at the higher concentration of nanoparticles. In vitro bioactivity assay performed in the simulated body fluid (SBF) indicated that composite PHBV scaffolds were able to induce the formation of apatite deposits after incubation in SBF. From the results of in vitro biological assay, it is concluded that the synergetic effect of BR and GPTMS provided an enhanced hFob cells attachment and proliferation. The developed PHBV/G-BR nanofibrous scaffolds may be considered for application in bone tissue engineering. Springer 2019-06-15 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25208/1/GPTMS-modified%20bredigite-PHBV%20nanofibrous%20bone%20scaffolds%20.pdf Kouhi, Monireh and Jayarama Reddy, Venugopal and Ramakrishna, Seeram (2019) GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties. Applied Biochemistry and Biotechnology, 188 (2). pp. 357-368. ISSN 0273-2289 https://doi.org/10.1007/s12010-018-2922-0 https://doi.org/10.1007/s12010-018-2922-0
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry R Medicine (General) RD Surgery TP Chemical technology
spellingShingle	QD Chemistry R Medicine (General) RD Surgery TP Chemical technology Kouhi, Monireh Jayarama Reddy, Venugopal Ramakrishna, Seeram GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties
description	Bioceramic nanoparticles with high specific surface area often tend to agglomerate in the polymer matrix, which results in undesirable mechanical properties of the composites and poor cell spreading and attachment. In the present work, bredigite (BR) nanoparticles were modified with an organosilane coupling agent, 3-glycidoxypropyltrimethoxysilane (GPTMS), to enhance its dispersibility in the polymer matrix. The polyhydroxybutyrate-co-hydroxyvaletare (PHBV) nanofibrous scaffolds containing either bredigite or GPTMS-modified bredigite (G-BR) nanoparticles were fabricated using electrospinning technique and characterized using scanning electron microscopy, transmission electron microscopy, and tensile strength. Results demonstrated that modification of bredigite was effective in enhancing nanoparticle dispersion in the PHBV matrix. PHBV/G-BR scaffold showed improved mechanical properties compared to PHBV and PHBV/BR, especially at the higher concentration of nanoparticles. In vitro bioactivity assay performed in the simulated body fluid (SBF) indicated that composite PHBV scaffolds were able to induce the formation of apatite deposits after incubation in SBF. From the results of in vitro biological assay, it is concluded that the synergetic effect of BR and GPTMS provided an enhanced hFob cells attachment and proliferation. The developed PHBV/G-BR nanofibrous scaffolds may be considered for application in bone tissue engineering.
format	Article
author	Kouhi, Monireh Jayarama Reddy, Venugopal Ramakrishna, Seeram
author_facet	Kouhi, Monireh Jayarama Reddy, Venugopal Ramakrishna, Seeram
author_sort	Kouhi, Monireh
title	GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties
title_short	GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties
title_full	GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties
title_fullStr	GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties
title_full_unstemmed	GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties
title_sort	gptms-modified bredigite/phbv nanofibrous bone scaffolds with enhanced mechanical and biological properties
publisher	Springer
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/25208/ http://umpir.ump.edu.my/id/eprint/25208/ http://umpir.ump.edu.my/id/eprint/25208/ http://umpir.ump.edu.my/id/eprint/25208/1/GPTMS-modified%20bredigite-PHBV%20nanofibrous%20bone%20scaffolds%20.pdf
first_indexed	2023-09-18T22:38:35Z
last_indexed	2023-09-18T22:38:35Z
_version_	1777416761812975616

GPTMS-modified bredigite/PHBV nanofibrous bone scaffolds with enhanced mechanical and biological properties

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