Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay

The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not ne...

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Main Authors:	Tsukada, Keiji, Tsunashima, Kenta, Jinno, Katsuya, Hiramatsu, Bunta, Takeuchi, Shun, Fujimoto, Kayo, Kenji, Sakai, Kiwa, Toshihiko, Mohd Mawardi, Saari
Format:	Article
Language:	English
Published:	IEEE 2019
Subjects:	Q Science (General) R Medicine (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/1/Using%20Magnetic%20Field%20Gradients%20to%20Shorten%20the%20Antigen1.pdf

id	ump-25077
recordtype	eprints
spelling	ump-250772019-07-01T04:54:50Z http://umpir.ump.edu.my/id/eprint/25077/ Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari Q Science (General) R Medicine (General) The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not need bound/free marker separation and the ability to detect biological targets in a nebulous liquid. In addition, the wash-free process is very fast. In this paper, we tried to reduce the reaction time for the antigen-antibody reaction in order to make the MIA method more attractive. The reduction in reaction time and the detection characteristic of biological targets were demonstrated using C-reactive proteins (CRPs) as biological targets for diagnosing inflammation. To shorten the reaction time, a magnetic-shaking method using the magnetic field gradient from a neodymium magnet was developed. When comparing the reaction times with and without magnetic shaking, it was found that the reaction time decreased with magnetic shaking for all CRP concentrations. Even after the magnetic-shaking treatment, the dependence of the CRP concentration on the magnetic signal was observed. The number of surface modifications per magnetic marker and the magnetic marker concentration also affected the reaction time. In order to obtain the desired measurement range based on the CRP response characteristic, it is necessary to optimize the number of magnetic markers and polymer beads used. IEEE 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25077/1/Using%20Magnetic%20Field%20Gradients%20to%20Shorten%20the%20Antigen1.pdf Tsukada, Keiji and Tsunashima, Kenta and Jinno, Katsuya and Hiramatsu, Bunta and Takeuchi, Shun and Fujimoto, Kayo and Kenji, Sakai and Kiwa, Toshihiko and Mohd Mawardi, Saari (2019) Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay. IEEE Transactions on Magnetics, 55 (7). pp. 1-5. ISSN 0018-9464 https://doi.org/10.1109/TMAG.2019.2894904 https://doi.org/10.1109/TMAG.2019.2894904
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) R Medicine (General)
spellingShingle	Q Science (General) R Medicine (General) Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
description	The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not need bound/free marker separation and the ability to detect biological targets in a nebulous liquid. In addition, the wash-free process is very fast. In this paper, we tried to reduce the reaction time for the antigen-antibody reaction in order to make the MIA method more attractive. The reduction in reaction time and the detection characteristic of biological targets were demonstrated using C-reactive proteins (CRPs) as biological targets for diagnosing inflammation. To shorten the reaction time, a magnetic-shaking method using the magnetic field gradient from a neodymium magnet was developed. When comparing the reaction times with and without magnetic shaking, it was found that the reaction time decreased with magnetic shaking for all CRP concentrations. Even after the magnetic-shaking treatment, the dependence of the CRP concentration on the magnetic signal was observed. The number of surface modifications per magnetic marker and the magnetic marker concentration also affected the reaction time. In order to obtain the desired measurement range based on the CRP response characteristic, it is necessary to optimize the number of magnetic markers and polymer beads used.
format	Article
author	Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari
author_facet	Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari
author_sort	Tsukada, Keiji
title	Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
title_short	Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
title_full	Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
title_fullStr	Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
title_full_unstemmed	Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
title_sort	using magnetic field gradients to shorten the antigen-antibody reaction time for a magnetic immunoassay
publisher	IEEE
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/1/Using%20Magnetic%20Field%20Gradients%20to%20Shorten%20the%20Antigen1.pdf
first_indexed	2023-09-18T22:38:19Z
last_indexed	2023-09-18T22:38:19Z
_version_	1777416745190948864

Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay

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