Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques

Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques

The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids,...

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Main Authors: Alara, Oluwaseun Ruth, Nour, A. H., Ishmael, Ukaegbu Chinonso, Kabbashi, Nassereldeen Ahmed
Format: Article
Language:English
Published: Taylor & Francis 2019
Subjects:
QD Chemistry
QK Botany
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/24723/
http://umpir.ump.edu.my/id/eprint/24723/
http://umpir.ump.edu.my/id/eprint/24723/
http://umpir.ump.edu.my/id/eprint/24723/1/Extraction%20and%20characterization%20of%20bioactive%20compounds%20in%20Vernonia.pdf
id ump-24723
recordtype eprints
spelling ump-247232020-02-21T07:34:21Z http://umpir.ump.edu.my/id/eprint/24723/ Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques Alara, Oluwaseun Ruth Nour, A. H. Ishmael, Ukaegbu Chinonso Kabbashi, Nassereldeen Ahmed QD Chemistry QK Botany TP Chemical technology The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GC-MS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique. Taylor & Francis 2019-12-11 Article PeerReviewed pdf en cc_by_nc_4 http://umpir.ump.edu.my/id/eprint/24723/1/Extraction%20and%20characterization%20of%20bioactive%20compounds%20in%20Vernonia.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Ishmael, Ukaegbu Chinonso and Kabbashi, Nassereldeen Ahmed (2019) Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. Journal of Taibah University for Science, 13 (1). pp. 414-422. ISSN 1658-3655 https://doi.org/10.1080/16583655.2019.1582460 https://doi.org/10.1080/16583655.2019.1582460
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QD Chemistry
QK Botany
TP Chemical technology
spellingShingle QD Chemistry
QK Botany
TP Chemical technology
Alara, Oluwaseun Ruth
Nour, A. H.
Ishmael, Ukaegbu Chinonso
Kabbashi, Nassereldeen Ahmed
Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
description The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GC-MS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique.
format Article
author Alara, Oluwaseun Ruth
Nour, A. H.
Ishmael, Ukaegbu Chinonso
Kabbashi, Nassereldeen Ahmed
author_facet Alara, Oluwaseun Ruth
Nour, A. H.
Ishmael, Ukaegbu Chinonso
Kabbashi, Nassereldeen Ahmed
author_sort Alara, Oluwaseun Ruth
title Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_short Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_full Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_fullStr Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_full_unstemmed Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
title_sort extraction and characterization of bioactive compounds in vernonia amygdalina leaf ethanolic extract comparing soxhlet and microwave-assisted extraction techniques
publisher Taylor & Francis
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/24723/
http://umpir.ump.edu.my/id/eprint/24723/
http://umpir.ump.edu.my/id/eprint/24723/
http://umpir.ump.edu.my/id/eprint/24723/1/Extraction%20and%20characterization%20of%20bioactive%20compounds%20in%20Vernonia.pdf
first_indexed 2023-09-18T22:37:35Z
last_indexed 2023-09-18T22:37:35Z
_version_ 1777416699373420544

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