Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line

Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improv...

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Main Author:	Wong, Shin Yie
Format:	Undergraduates Project Papers
Language:	English English English English
Published:	2018
Subjects:	T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/24535/ http://umpir.ump.edu.my/id/eprint/24535/ http://umpir.ump.edu.my/id/eprint/24535/1/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/24535/2/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/24535/3/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/24535/4/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20References.pdf

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recordtype	eprints
spelling	ump-245352019-05-29T05:17:49Z http://umpir.ump.edu.my/id/eprint/24535/ Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line Wong, Shin Yie T Technology (General) Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential. 2018-01 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24535/1/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/24535/2/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/24535/3/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/24535/4/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20References.pdf Wong, Shin Yie (2018) Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line. Faculty of Engineering Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:103753&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English English
topic	T Technology (General)
spellingShingle	T Technology (General) Wong, Shin Yie Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
description	Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential.
format	Undergraduates Project Papers
author	Wong, Shin Yie
author_facet	Wong, Shin Yie
author_sort	Wong, Shin Yie
title	Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_short	Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_full	Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_fullStr	Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_full_unstemmed	Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_sort	statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/24535/ http://umpir.ump.edu.my/id/eprint/24535/ http://umpir.ump.edu.my/id/eprint/24535/1/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/24535/2/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/24535/3/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/24535/4/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20References.pdf
first_indexed	2023-09-18T22:37:11Z
last_indexed	2023-09-18T22:37:11Z
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Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line

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