Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology

Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme which undergoes cyclization reaction to converts starch into cyclodextrin (CD). Due to their potential properties, CD has been discovered to have numerous application in food industries, pharmaceuticals, agriculture and...

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Main Authors:	Rohaida, Che Man, Natassha, Jamil, S., Suhaimi, Shalyda, Md Shaarani, Zatul Iffah, Mohd Arshad, Siti Kholijah, Abdul Mudalip, Siti Zubaidah, Sulaiman
Format:	Conference or Workshop Item
Language:	English English
Published:	IOP Publishing 2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/24268/ http://umpir.ump.edu.my/id/eprint/24268/ http://umpir.ump.edu.my/id/eprint/24268/1/42.%20Immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf http://umpir.ump.edu.my/id/eprint/24268/2/42.1%20%20Immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf

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recordtype	eprints
spelling	ump-242682019-08-22T04:02:32Z http://umpir.ump.edu.my/id/eprint/24268/ Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology Rohaida, Che Man Natassha, Jamil S., Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman TP Chemical technology Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme which undergoes cyclization reaction to converts starch into cyclodextrin (CD). Due to their potential properties, CD has been discovered to have numerous application in food industries, pharmaceuticals, agriculture and environmental engineering. However, the instability of the enzyme during the reaction process result in the low production of CD. Thus, enzyme immobilization process has been used to improve the enzyme stability in order to achieve high production of CD. In this study, CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane via physical adsorption. The optimization of the immobilization parameters and the performance of the immobilized CGTase were investigated. The adsorption of CGTase on hollow fiber membrane was evaluated by fourier transform infrared spectroscopy (FTIR). Response surface methodology (RSM) was employed to optimize enzyme immobilization by manipulating the immobilization parameters of contact time (15-33 h), immobilization pH (pH 6-8) and immobilization temperature (20-30 °C) on the immobilization yield. The optimized immobilization conditions were 24 °C of immobilization temperature, pH 6.7 and 24 h of contact time, with 88.25% of immobilization yield. Immobilization of CGTase on the hollow fiber membrane was successfully optimized and about 4.6-fold increment of immobilization yield was achieved after the optimization process. Reusability of the immobilized CGTase revealed that the immobilized enzyme could retain 37.7% of its initial activity after the 10th cycles. The cumulative production of CD by the immobilized CGTase after 10 cycles was 26.43 mg/ml. The kinetic parameters of the immobilized CGTase were 9.42 mgml-1 h-1 and 9.99 mg ml-1 for Vmax and Km value, respectively. The kinetic studies revealed that the catalytic efficiency of the immobilized CGTase was similar to the free CGTase, demonstrated that upon the immobilization process, adsorption of CGTase on hollow fiber membrane does not cause structural changes to the enzyme. Hence, immobilization of CGTase on the hollow fiber membrane substantially improved the production of CD and suggesting that the hollow fiber membrane appeared as a suitable support for the enzyme immobilization system. IOP Publishing 2019-07 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24268/1/42.%20Immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf pdf en http://umpir.ump.edu.my/id/eprint/24268/2/42.1%20%20Immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf Rohaida, Che Man and Natassha, Jamil and S., Suhaimi and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman (2019) Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology. In: International Conference On Sustainable Energy And Green Technology 2018, 11-14 Disember 2018 , Double Tree By Hilton Hotel Kuala Lumpur. pp. 1-8., 268 (1). ISSN 1755-1307 https://iopscience.iop.org/article/10.1088/1755-1315/268/1/012008/pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rohaida, Che Man Natassha, Jamil S., Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
description	Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme which undergoes cyclization reaction to converts starch into cyclodextrin (CD). Due to their potential properties, CD has been discovered to have numerous application in food industries, pharmaceuticals, agriculture and environmental engineering. However, the instability of the enzyme during the reaction process result in the low production of CD. Thus, enzyme immobilization process has been used to improve the enzyme stability in order to achieve high production of CD. In this study, CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane via physical adsorption. The optimization of the immobilization parameters and the performance of the immobilized CGTase were investigated. The adsorption of CGTase on hollow fiber membrane was evaluated by fourier transform infrared spectroscopy (FTIR). Response surface methodology (RSM) was employed to optimize enzyme immobilization by manipulating the immobilization parameters of contact time (15-33 h), immobilization pH (pH 6-8) and immobilization temperature (20-30 °C) on the immobilization yield. The optimized immobilization conditions were 24 °C of immobilization temperature, pH 6.7 and 24 h of contact time, with 88.25% of immobilization yield. Immobilization of CGTase on the hollow fiber membrane was successfully optimized and about 4.6-fold increment of immobilization yield was achieved after the optimization process. Reusability of the immobilized CGTase revealed that the immobilized enzyme could retain 37.7% of its initial activity after the 10th cycles. The cumulative production of CD by the immobilized CGTase after 10 cycles was 26.43 mg/ml. The kinetic parameters of the immobilized CGTase were 9.42 mgml-1 h-1 and 9.99 mg ml-1 for Vmax and Km value, respectively. The kinetic studies revealed that the catalytic efficiency of the immobilized CGTase was similar to the free CGTase, demonstrated that upon the immobilization process, adsorption of CGTase on hollow fiber membrane does not cause structural changes to the enzyme. Hence, immobilization of CGTase on the hollow fiber membrane substantially improved the production of CD and suggesting that the hollow fiber membrane appeared as a suitable support for the enzyme immobilization system.
format	Conference or Workshop Item
author	Rohaida, Che Man Natassha, Jamil S., Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman
author_facet	Rohaida, Che Man Natassha, Jamil S., Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman
author_sort	Rohaida, Che Man
title	Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
title_short	Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
title_full	Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
title_fullStr	Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
title_full_unstemmed	Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology
title_sort	immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: optimization of the immobilization parameters by response surface methodology
publisher	IOP Publishing
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/24268/ http://umpir.ump.edu.my/id/eprint/24268/ http://umpir.ump.edu.my/id/eprint/24268/1/42.%20Immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf http://umpir.ump.edu.my/id/eprint/24268/2/42.1%20%20Immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf
first_indexed	2023-09-18T22:36:37Z
last_indexed	2023-09-18T22:36:37Z
_version_	1777416638377754624

Immobilization of cyclodextrin glucanotransferase on hollow fiber membrane: Optimization of the immobilization parameters by response surface methodology

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