Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture
Hepatitis B virus‐like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow‐through chromatography mode. The virus‐like particles were selectively excluded, while the relatively smaller sized host...
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WILEY-VCH Verlag
2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/23913/ http://umpir.ump.edu.my/id/eprint/23913/ http://umpir.ump.edu.my/id/eprint/23913/ http://umpir.ump.edu.my/id/eprint/23913/1/size%20selective%20purification.pdf |
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ump-239132019-02-11T04:17:53Z http://umpir.ump.edu.my/id/eprint/23913/ Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture Ng, Hon Wei Lee, Micky Fu Xiang Chua, Gek Kee Gan, Bee Koon Tan, Wen Siang Ooi, Chien Wei Tang, Siah Ying Chan, Eng Seng Tey, Beng Ti TP Chemical technology Hepatitis B virus‐like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow‐through chromatography mode. The virus‐like particles were selectively excluded, while the relatively smaller sized host cell proteins were absorbed. The exclusion of virus‐like particles was governed by the accessibility of binding sites (the size of adsorbents and the charge of grafted dextran chains) as well as the architecture (branch‐chain length) of the grafted polymer. The branch‐chain length of grafted polymer was altered by changing the type of monomers used. The larger adsorbent (90 μm) had an approximately twofold increase in the flow‐through recovery, as compared to the smaller adsorbent (30 μm). Generally, polymer‐grafted adsorbents improved the exclusion of the virus‐like particles. Overall, the middle branch‐chain length polymer grafted on larger adsorbent showed optimal performance at 92% flow‐through recovery with a purification factor of 1.53. A comparative study between the adsorbent with dextran grafts and the polymer‐grafted adsorbent showed that a better exclusion of virus‐like particles was achieved with the absorbent grafted with inert polymer. The grafted polymer was also shown to reduce strong interaction between binding sites and virus‐like particles, which preserved the particles’ structure. WILEY-VCH Verlag 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23913/1/size%20selective%20purification.pdf Ng, Hon Wei and Lee, Micky Fu Xiang and Chua, Gek Kee and Gan, Bee Koon and Tan, Wen Siang and Ooi, Chien Wei and Tang, Siah Ying and Chan, Eng Seng and Tey, Beng Ti (2018) Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture. Journal of Separation Science, 41 (10). pp. 2119-2129. ISSN 1615-9314 https://doi.org/10.1002/jssc.201700823 https://doi.org/10.1002/jssc.201700823 |
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Digital Repository |
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Local University |
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Universiti Malaysia Pahang |
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UMP Institutional Repository |
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Online Access |
| language |
English |
| topic |
TP Chemical technology |
| spellingShingle |
TP Chemical technology Ng, Hon Wei Lee, Micky Fu Xiang Chua, Gek Kee Gan, Bee Koon Tan, Wen Siang Ooi, Chien Wei Tang, Siah Ying Chan, Eng Seng Tey, Beng Ti Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture |
| description |
Hepatitis B virus‐like particles expressed in Escherichia coli were purified using anion exchange adsorbents grafted with polymer poly(oligo(ethylene glycol) methacrylate) in flow‐through chromatography mode. The virus‐like particles were selectively excluded, while the relatively smaller sized host cell proteins were absorbed. The exclusion of virus‐like particles was governed by the accessibility of binding sites (the size of adsorbents and the charge of grafted dextran chains) as well as the architecture (branch‐chain length) of the grafted polymer. The branch‐chain length of grafted polymer was altered by changing the type of monomers used. The larger adsorbent (90 μm) had an approximately twofold increase in the flow‐through recovery, as compared to the smaller adsorbent (30 μm). Generally, polymer‐grafted adsorbents improved the exclusion of the virus‐like particles. Overall, the middle branch‐chain length polymer grafted on larger adsorbent showed optimal performance at 92% flow‐through recovery with a purification factor of 1.53. A comparative study between the adsorbent with dextran grafts and the polymer‐grafted adsorbent showed that a better exclusion of virus‐like particles was achieved with the absorbent grafted with inert polymer. The grafted polymer was also shown to reduce strong interaction between binding sites and virus‐like particles, which preserved the particles’ structure. |
| format |
Article |
| author |
Ng, Hon Wei Lee, Micky Fu Xiang Chua, Gek Kee Gan, Bee Koon Tan, Wen Siang Ooi, Chien Wei Tang, Siah Ying Chan, Eng Seng Tey, Beng Ti |
| author_facet |
Ng, Hon Wei Lee, Micky Fu Xiang Chua, Gek Kee Gan, Bee Koon Tan, Wen Siang Ooi, Chien Wei Tang, Siah Ying Chan, Eng Seng Tey, Beng Ti |
| author_sort |
Ng, Hon Wei |
| title |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture |
| title_short |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture |
| title_full |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture |
| title_fullStr |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture |
| title_full_unstemmed |
Size-selective purification of hepatitis B virus-like particle in flow-through chromatography: Types of ion exchange adsorbent and grafted polymer architecture |
| title_sort |
size-selective purification of hepatitis b virus-like particle in flow-through chromatography: types of ion exchange adsorbent and grafted polymer architecture |
| publisher |
WILEY-VCH Verlag |
| publishDate |
2018 |
| url |
http://umpir.ump.edu.my/id/eprint/23913/ http://umpir.ump.edu.my/id/eprint/23913/ http://umpir.ump.edu.my/id/eprint/23913/ http://umpir.ump.edu.my/id/eprint/23913/1/size%20selective%20purification.pdf |
| first_indexed |
2023-09-18T22:36:01Z |
| last_indexed |
2023-09-18T22:36:01Z |
| _version_ |
1777416600741216256 |