Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane

Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been pro...

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Main Authors:	Natassha, Jamil, Rohaida, Che Man, Suhaily, Suhaimi, Shalyda, Md Shaarani, Zatul Iffah, Mohd Arshad, Siti Kholijah, Abdul Mudalip, Siti Zubaidah, Sulaiman
Format:	Conference or Workshop Item
Language:	English English
Published:	Elsevier Ltd 2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/1/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf http://umpir.ump.edu.my/id/eprint/23883/7/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase.pdf

id	ump-23883
recordtype	eprints
spelling	ump-238832019-05-17T04:00:43Z http://umpir.ump.edu.my/id/eprint/23883/ Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Q Science (General) Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been proven as a promising method to improve the enzyme stability in order to achieve the high production of CD. In this study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption. The effects of the enzyme concentration [0.6-1.4% (v/v)] and temperature (20-40 °C) on the immobilization yield were investigated. The highest immobilization yield of 80.0% was observed when 10% (v/v) of enzyme concentration was used. The optimal immobilization temperature obtained was at 25 °C with 95.6% of immobilization yield. Hence, the high immobilization yield contributed to the high production of CD which may be beneficial for the industrial purposes. Elsevier Ltd 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23883/1/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf pdf en http://umpir.ump.edu.my/id/eprint/23883/7/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase.pdf Natassha, Jamil and Rohaida, Che Man and Suhaily, Suhaimi and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman (2018) Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane. In: The 3rd International Conference on Green Chemical Engineering and Technology (3rd GCET), 7-8 November 2017 , Melaka, Malaysia. pp. 22036-22042., 5 (10). ISSN 2214-7853 https://doi.org/10.1016/j.matpr.2018.07.065 10.1016/j.matpr.2018.07.065
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
description	Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been proven as a promising method to improve the enzyme stability in order to achieve the high production of CD. In this study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption. The effects of the enzyme concentration [0.6-1.4% (v/v)] and temperature (20-40 °C) on the immobilization yield were investigated. The highest immobilization yield of 80.0% was observed when 10% (v/v) of enzyme concentration was used. The optimal immobilization temperature obtained was at 25 °C with 95.6% of immobilization yield. Hence, the high immobilization yield contributed to the high production of CD which may be beneficial for the industrial purposes.
format	Conference or Workshop Item
author	Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman
author_facet	Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman
author_sort	Natassha, Jamil
title	Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
title_short	Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
title_full	Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
title_fullStr	Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
title_full_unstemmed	Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
title_sort	effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (cgtase) on hollow fiber membrane
publisher	Elsevier Ltd
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/1/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf http://umpir.ump.edu.my/id/eprint/23883/7/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase.pdf
first_indexed	2023-09-18T22:35:58Z
last_indexed	2023-09-18T22:35:58Z
_version_	1777416597487484928

Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane

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