Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx

This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method f...

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Main Authors:	A. K. M., Kafi, Rajan, Jose, M. M., Yusoff, Samiul, Alim
Format:	Article
Language:	English
Published:	American Scientific Publishers 2019
Subjects:	T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/1/Application%20of%20polymerized%20multiporous%20nanofiber%20of%20SnO2%20for%20designing.pdf

id	ump-23537
recordtype	eprints
spelling	ump-235372019-01-29T01:26:37Z http://umpir.ump.edu.my/id/eprint/23537/ Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim T Technology (General) This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor which possesses high surface area good electrical conductivity, and the nanofibers were polymerized with polyaniline (PANI). GOx and HRP were then co-immobilized with the nanofibers on the surface of the glassy carbon electrode by using chitosan. The polymerized nanofibers play a significant role in facilitating the direct electron transfer between the electroactive center of the immobilized enzyme and the electrode surface. The morphology of the nanofiber and polymerized nanofiber has been evaluated by field emission scanning electron microscopy (FESEM). Cyclic Voltammetry and amperometry were employed to study and optimize the performance of the fabricated biosensor. The PANI/SnO2-NF/GOx-HRP/Ch/GC biosensor displayed a linear amperometric response towards the glucose concentration range from 5 to 100 μM with a detection limit of 1.8 μM (S/N = 3). Also, the anti-interference study and real sample analysis was investigated. Furthermore, the biosensor reported in this work exhibited excellent stability, reproducibility, and repeatability American Scientific Publishers 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23537/1/Application%20of%20polymerized%20multiporous%20nanofiber%20of%20SnO2%20for%20designing.pdf A. K. M., Kafi and Rajan, Jose and M. M., Yusoff and Samiul, Alim (2019) Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx. International Journal of Biological Macromolecules, 123. pp. 1028-1034. http://www.elsevier.com/locate/ijbiomac https://doi.org/10.1166/jnn.2019.15465
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
description	This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor which possesses high surface area good electrical conductivity, and the nanofibers were polymerized with polyaniline (PANI). GOx and HRP were then co-immobilized with the nanofibers on the surface of the glassy carbon electrode by using chitosan. The polymerized nanofibers play a significant role in facilitating the direct electron transfer between the electroactive center of the immobilized enzyme and the electrode surface. The morphology of the nanofiber and polymerized nanofiber has been evaluated by field emission scanning electron microscopy (FESEM). Cyclic Voltammetry and amperometry were employed to study and optimize the performance of the fabricated biosensor. The PANI/SnO2-NF/GOx-HRP/Ch/GC biosensor displayed a linear amperometric response towards the glucose concentration range from 5 to 100 μM with a detection limit of 1.8 μM (S/N = 3). Also, the anti-interference study and real sample analysis was investigated. Furthermore, the biosensor reported in this work exhibited excellent stability, reproducibility, and repeatability
format	Article
author	A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim
author_facet	A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim
author_sort	A. K. M., Kafi
title	Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
title_short	Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
title_full	Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
title_fullStr	Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
title_full_unstemmed	Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
title_sort	application of polymerized multiporous nanofiber of sno2 for designing a bienzyme glucose biosensor based on hrp/gox
publisher	American Scientific Publishers
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/1/Application%20of%20polymerized%20multiporous%20nanofiber%20of%20SnO2%20for%20designing.pdf
first_indexed	2023-09-18T22:35:17Z
last_indexed	2023-09-18T22:35:17Z
_version_	1777416554508451840

Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx

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