3D extracellular morphology features from rapid photo-polymerized cell replica

Morphology features of cells plays a vital role in cell research, drug delivery, diagnostic, therapeutic and many other applications. In this paper we report a method of morphology feature extraction from the cell replica. Cell imprinting is a soft lithography technique used to obtain the replica of...

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Main Authors:	Vigneswaran, Narayanamurthy, Fahmi, Samsuri, Kalyani, K. Nithya
Format:	Article
Language:	English
Published:	American Scientific Publishers 2018
Subjects:	TK Electrical engineering. Electronics Nuclear engineering
Online Access:	http://umpir.ump.edu.my/id/eprint/23375/ http://umpir.ump.edu.my/id/eprint/23375/1/Extracellular%20morphology%20features%20from%20rapid%20photo-polymerized%20cell%20replica.pdf

id	ump-23375
recordtype	eprints
spelling	ump-233752019-01-02T01:33:12Z http://umpir.ump.edu.my/id/eprint/23375/ 3D extracellular morphology features from rapid photo-polymerized cell replica Vigneswaran, Narayanamurthy Fahmi, Samsuri Kalyani, K. Nithya TK Electrical engineering. Electronics Nuclear engineering Morphology features of cells plays a vital role in cell research, drug delivery, diagnostic, therapeutic and many other applications. In this paper we report a method of morphology feature extraction from the cell replica. Cell imprinting is a soft lithography technique used to obtain the replica of cell morphology. Morphology features like shape and size of cell, shape and size of nucleus, pores in cell membrane can be imaged comparatively with minimal noise or without any noise and the same can be detected using this technique. This technique helps to investigate the shape of grooves, pores, blebs or microvillus on the cellular surface and helps in better diagnosis and analysis at single cell level. However conventional fixation, sectioning and viewing under scanning electron microscope (SEM) or transmission electron microscope (TEM) can provide many insights more clearly although the process is quite complex and tedious. The main finding of this research is that we developed an imaging technique for the cells, which can provide morphology information on single cell sub organelle scale in much detail. Atomic force microscope (AFM) in tapping mode was used to image the replica. Technique also delivers a 3-D image of the cell along with its complete morphological details. The limitation of this technique is that, it only provides the morphology information. Thus abnormalities which doesn’t designate on morphology cannot be diagnosed. This technique finds its application where single cells are to be analyzed and diagnosis for study based on morphology, especially for drug delivery applications and for investigations based on molecular pathways. As a future prospective, morphology features obtained through this technique can also be used to train the artificial neural network for decision making completely based on this technique. American Scientific Publishers 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23375/1/Extracellular%20morphology%20features%20from%20rapid%20photo-polymerized%20cell%20replica.pdf Vigneswaran, Narayanamurthy and Fahmi, Samsuri and Kalyani, K. Nithya (2018) 3D extracellular morphology features from rapid photo-polymerized cell replica. Advanced Science, Engineering and Medicine. pp. 1-7. ISSN 2164-6627 (In Press)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TK Electrical engineering. Electronics Nuclear engineering
spellingShingle	TK Electrical engineering. Electronics Nuclear engineering Vigneswaran, Narayanamurthy Fahmi, Samsuri Kalyani, K. Nithya 3D extracellular morphology features from rapid photo-polymerized cell replica
description	Morphology features of cells plays a vital role in cell research, drug delivery, diagnostic, therapeutic and many other applications. In this paper we report a method of morphology feature extraction from the cell replica. Cell imprinting is a soft lithography technique used to obtain the replica of cell morphology. Morphology features like shape and size of cell, shape and size of nucleus, pores in cell membrane can be imaged comparatively with minimal noise or without any noise and the same can be detected using this technique. This technique helps to investigate the shape of grooves, pores, blebs or microvillus on the cellular surface and helps in better diagnosis and analysis at single cell level. However conventional fixation, sectioning and viewing under scanning electron microscope (SEM) or transmission electron microscope (TEM) can provide many insights more clearly although the process is quite complex and tedious. The main finding of this research is that we developed an imaging technique for the cells, which can provide morphology information on single cell sub organelle scale in much detail. Atomic force microscope (AFM) in tapping mode was used to image the replica. Technique also delivers a 3-D image of the cell along with its complete morphological details. The limitation of this technique is that, it only provides the morphology information. Thus abnormalities which doesn’t designate on morphology cannot be diagnosed. This technique finds its application where single cells are to be analyzed and diagnosis for study based on morphology, especially for drug delivery applications and for investigations based on molecular pathways. As a future prospective, morphology features obtained through this technique can also be used to train the artificial neural network for decision making completely based on this technique.
format	Article
author	Vigneswaran, Narayanamurthy Fahmi, Samsuri Kalyani, K. Nithya
author_facet	Vigneswaran, Narayanamurthy Fahmi, Samsuri Kalyani, K. Nithya
author_sort	Vigneswaran, Narayanamurthy
title	3D extracellular morphology features from rapid photo-polymerized cell replica
title_short	3D extracellular morphology features from rapid photo-polymerized cell replica
title_full	3D extracellular morphology features from rapid photo-polymerized cell replica
title_fullStr	3D extracellular morphology features from rapid photo-polymerized cell replica
title_full_unstemmed	3D extracellular morphology features from rapid photo-polymerized cell replica
title_sort	3d extracellular morphology features from rapid photo-polymerized cell replica
publisher	American Scientific Publishers
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23375/ http://umpir.ump.edu.my/id/eprint/23375/1/Extracellular%20morphology%20features%20from%20rapid%20photo-polymerized%20cell%20replica.pdf
first_indexed	2023-09-18T22:34:57Z
last_indexed	2023-09-18T22:34:57Z
_version_	1777416533242281984

3D extracellular morphology features from rapid photo-polymerized cell replica

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