Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells

A nanoporous carbon (NPC) was synthesized from Oil palm leaves (OPL) by pyrolysis at 600 °C in a N2 atmosphere, oxidized by a mixture of sulfuric and nitric acids (3:1 vol/vol) and conjugated with coumarin-6 as a fluorescence dye for cellular imaging and drug delivery to cancer cells. The structure,...

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Main Authors:	Yallappa, S., Shoriya Aruni, Abdul Manaf, Hegde, Gurumurthy
Format:	Article
Language:	English
Published:	Elsevier Ltd. 2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/23268/ http://umpir.ump.edu.my/id/eprint/23268/ http://umpir.ump.edu.my/id/eprint/23268/ http://umpir.ump.edu.my/id/eprint/23268/1/Synthesis%20of%20a%20biocompatible%20nanoporous%20carbon%20and%20its%20conjugation%20with%20florescent%20dye%20for%20cellular%20imaging%20and%20targeted%20drug%20delivery%20to%20cancer%20cells.pdf

id	ump-23268
recordtype	eprints
spelling	ump-232682019-02-25T03:45:49Z http://umpir.ump.edu.my/id/eprint/23268/ Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells Yallappa, S. Shoriya Aruni, Abdul Manaf Hegde, Gurumurthy Q Science (General) A nanoporous carbon (NPC) was synthesized from Oil palm leaves (OPL) by pyrolysis at 600 °C in a N2 atmosphere, oxidized by a mixture of sulfuric and nitric acids (3:1 vol/vol) and conjugated with coumarin-6 as a fluorescence dye for cellular imaging and drug delivery to cancer cells. The structure, morphology and dispersion stability of NPC in aqueous media before and after the conjugation were investigated by XRD, FT-IR, Raman spectroscopy, SEM, TEM and zeta potential measurement. Cell uptake for the conjugated NPC was investigated by fluorescence microscopy. Results indicate that the cellulose, hemicelluloses and lignin in OPL convert to a graphitic structure by pyrolysis. NPC consists of spherical nanoparticles with diameters of 30–50 nm and has a high graphitic content with an ID/IG ratio of 0.7. The coumarin-6 is successfully conjugated to NPC by forming a complex. Both NPC and the conjugated NPC have a high dispersion stability in aqueous media. NPC is biocompatible with a negligible cytotoxicity. The conjugated NPC exhibits a high cell uptake, is highly biocompatible for normal cells and toxic for tested human cancer cells. NPC is a good candidate for cellular imaging and targeted drug delivery. Elsevier Ltd. 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23268/1/Synthesis%20of%20a%20biocompatible%20nanoporous%20carbon%20and%20its%20conjugation%20with%20florescent%20dye%20for%20cellular%20imaging%20and%20targeted%20drug%20delivery%20to%20cancer%20cells.pdf Yallappa, S. and Shoriya Aruni, Abdul Manaf and Hegde, Gurumurthy (2018) Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells. New Carbon Materials, 33 (2). pp. 162-172. ISSN 1007-8827 https://doi.org/10.1016/S1872-5805(18)60332-4 https://doi.org/10.1016/S1872-5805(18)60332-4
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Yallappa, S. Shoriya Aruni, Abdul Manaf Hegde, Gurumurthy Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
description	A nanoporous carbon (NPC) was synthesized from Oil palm leaves (OPL) by pyrolysis at 600 °C in a N2 atmosphere, oxidized by a mixture of sulfuric and nitric acids (3:1 vol/vol) and conjugated with coumarin-6 as a fluorescence dye for cellular imaging and drug delivery to cancer cells. The structure, morphology and dispersion stability of NPC in aqueous media before and after the conjugation were investigated by XRD, FT-IR, Raman spectroscopy, SEM, TEM and zeta potential measurement. Cell uptake for the conjugated NPC was investigated by fluorescence microscopy. Results indicate that the cellulose, hemicelluloses and lignin in OPL convert to a graphitic structure by pyrolysis. NPC consists of spherical nanoparticles with diameters of 30–50 nm and has a high graphitic content with an ID/IG ratio of 0.7. The coumarin-6 is successfully conjugated to NPC by forming a complex. Both NPC and the conjugated NPC have a high dispersion stability in aqueous media. NPC is biocompatible with a negligible cytotoxicity. The conjugated NPC exhibits a high cell uptake, is highly biocompatible for normal cells and toxic for tested human cancer cells. NPC is a good candidate for cellular imaging and targeted drug delivery.
format	Article
author	Yallappa, S. Shoriya Aruni, Abdul Manaf Hegde, Gurumurthy
author_facet	Yallappa, S. Shoriya Aruni, Abdul Manaf Hegde, Gurumurthy
author_sort	Yallappa, S.
title	Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
title_short	Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
title_full	Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
title_fullStr	Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
title_full_unstemmed	Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
title_sort	synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells
publisher	Elsevier Ltd.
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23268/ http://umpir.ump.edu.my/id/eprint/23268/ http://umpir.ump.edu.my/id/eprint/23268/ http://umpir.ump.edu.my/id/eprint/23268/1/Synthesis%20of%20a%20biocompatible%20nanoporous%20carbon%20and%20its%20conjugation%20with%20florescent%20dye%20for%20cellular%20imaging%20and%20targeted%20drug%20delivery%20to%20cancer%20cells.pdf
first_indexed	2023-09-18T22:34:46Z
last_indexed	2023-09-18T22:34:46Z
_version_	1777416521630351360

Synthesis of a biocompatible nanoporous carbon and its conjugation with florescent dye for cellular imaging and targeted drug delivery to cancer cells

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