A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants

Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG in order to reduce the dependency of using animals as major sources. Methods: Crude GAG was extracted from four plants (G...

Full description

Bibliographic Details
Main Authors:	Che Nur Mazadillina, Zahari, Marina, Mohd Sham, Sakina, Shahabudin, Mohd Hairul, Ab. Rahim, Nina Suhaity, Azmi
Format:	Article
Language:	English
Published:	Innovare Academic Sciences Pvt Ltd 2019
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/23246/ http://umpir.ump.edu.my/id/eprint/23246/ http://umpir.ump.edu.my/id/eprint/23246/ http://umpir.ump.edu.my/id/eprint/23246/1/A%20rapid%20quantitative%20dye-binding%20method.pdf

id	ump-23246
recordtype	eprints
spelling	ump-232462019-02-25T04:19:57Z http://umpir.ump.edu.my/id/eprint/23246/ A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants Che Nur Mazadillina, Zahari Marina, Mohd Sham Sakina, Shahabudin Mohd Hairul, Ab. Rahim Nina Suhaity, Azmi Q Science (General) Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG in order to reduce the dependency of using animals as major sources. Methods: Crude GAG was extracted from four plants (Gaultheria procumbens, Strobilanthes crispus, Orthosiphon stamineus and Ficus deltoidea) using hot water extraction with some modifications. Ultraviolet (UV) spectrophotometry was conducted for purity test. Total sulfated GAG was determined by using Blyscan assay kit. By comparing results between the extract yields and total sulfated GAG, the plant consisting of high total sulfated GAG was chosen for further characterization. The selected plant sample was examined by microscopy and further analyzed by nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR) spectroscopy. Results: All four plants showed absorbance peaks between 214 and 232 nm in UV scan that represented negatively charged sugar. O. stamineus was found to contain the highest amount of sulfated GAG, 62.63 ± 0.01 µg/mg by Blyscan assay. Microscopical examination confirmed the identity of O. stamineus sample by comparing to the reference. Both NMR and FTIR analysis of O. stamineus crude yield showed presence of hydroxyl, sulfates, carboxylate, and amine groups, suggesting close resemblances to GAG structure. Conclusion: The results suggested that all four plants contained GAG compound. O. stamineus was found to exhibit the most abundant total sulfated GAG, and has potential to become a new plant-based source for GAG. Innovare Academic Sciences Pvt Ltd 2019-01 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/23246/1/A%20rapid%20quantitative%20dye-binding%20method.pdf Che Nur Mazadillina, Zahari and Marina, Mohd Sham and Sakina, Shahabudin and Mohd Hairul, Ab. Rahim and Nina Suhaity, Azmi (2019) A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants. Asian Journal of Pharmaceutical and Clinical Research, 12 (1). pp. 396-402. ISSN 0974-2441(print); 2455-3891(online) https://doi.org/10.22159/ajpcr.2019.v12i1.30283 https://doi.org/10.22159/ajpcr.2019.v12i1.30283
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Che Nur Mazadillina, Zahari Marina, Mohd Sham Sakina, Shahabudin Mohd Hairul, Ab. Rahim Nina Suhaity, Azmi A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
description	Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG in order to reduce the dependency of using animals as major sources. Methods: Crude GAG was extracted from four plants (Gaultheria procumbens, Strobilanthes crispus, Orthosiphon stamineus and Ficus deltoidea) using hot water extraction with some modifications. Ultraviolet (UV) spectrophotometry was conducted for purity test. Total sulfated GAG was determined by using Blyscan assay kit. By comparing results between the extract yields and total sulfated GAG, the plant consisting of high total sulfated GAG was chosen for further characterization. The selected plant sample was examined by microscopy and further analyzed by nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR) spectroscopy. Results: All four plants showed absorbance peaks between 214 and 232 nm in UV scan that represented negatively charged sugar. O. stamineus was found to contain the highest amount of sulfated GAG, 62.63 ± 0.01 µg/mg by Blyscan assay. Microscopical examination confirmed the identity of O. stamineus sample by comparing to the reference. Both NMR and FTIR analysis of O. stamineus crude yield showed presence of hydroxyl, sulfates, carboxylate, and amine groups, suggesting close resemblances to GAG structure. Conclusion: The results suggested that all four plants contained GAG compound. O. stamineus was found to exhibit the most abundant total sulfated GAG, and has potential to become a new plant-based source for GAG.
format	Article
author	Che Nur Mazadillina, Zahari Marina, Mohd Sham Sakina, Shahabudin Mohd Hairul, Ab. Rahim Nina Suhaity, Azmi
author_facet	Che Nur Mazadillina, Zahari Marina, Mohd Sham Sakina, Shahabudin Mohd Hairul, Ab. Rahim Nina Suhaity, Azmi
author_sort	Che Nur Mazadillina, Zahari
title	A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_short	A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_full	A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_fullStr	A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_full_unstemmed	A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_sort	rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
publisher	Innovare Academic Sciences Pvt Ltd
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/23246/ http://umpir.ump.edu.my/id/eprint/23246/ http://umpir.ump.edu.my/id/eprint/23246/ http://umpir.ump.edu.my/id/eprint/23246/1/A%20rapid%20quantitative%20dye-binding%20method.pdf
first_indexed	2023-09-18T22:34:43Z
last_indexed	2023-09-18T22:34:43Z
_version_	1777416518678609920

A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants

Similar Items