Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 H...

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Main Authors:	Karim, Ahasanul, Yousuf, Abu, M. Amirul, Islam, H. Naif, Yasir, C. K. M., Faizal, Alam, Md. Zahangir, Pirozzi, Domenico
Format:	Article
Language:	English
Published:	John Wiley and Sons Inc. 2018
Subjects:	T Technology (General) TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/22932/ http://umpir.ump.edu.my/id/eprint/22932/ http://umpir.ump.edu.my/id/eprint/22932/ http://umpir.ump.edu.my/id/eprint/22932/1/Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20using%20irreversible%20electroporation.pdf

id	ump-22932
recordtype	eprints
spelling	ump-229322019-10-18T02:29:32Z http://umpir.ump.edu.my/id/eprint/22932/ Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation Karim, Ahasanul Yousuf, Abu M. Amirul, Islam H. Naif, Yasir C. K. M., Faizal Alam, Md. Zahangir Pirozzi, Domenico T Technology (General) TP Chemical technology The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m−3). At this condition, maximum lipid (63 mg g−1) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. John Wiley and Sons Inc. 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22932/1/Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20using%20irreversible%20electroporation.pdf Karim, Ahasanul and Yousuf, Abu and M. Amirul, Islam and H. Naif, Yasir and C. K. M., Faizal and Alam, Md. Zahangir and Pirozzi, Domenico (2018) Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation. Biotechnology Progress, 34 (4). pp. 838-845. ISSN 1520-6033 https://doi.org/10.1002/btpr.2625 doi: 10.1002/btpr.2625
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	T Technology (General) TP Chemical technology
spellingShingle	T Technology (General) TP Chemical technology Karim, Ahasanul Yousuf, Abu M. Amirul, Islam H. Naif, Yasir C. K. M., Faizal Alam, Md. Zahangir Pirozzi, Domenico Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
description	The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m−3). At this condition, maximum lipid (63 mg g−1) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass.
format	Article
author	Karim, Ahasanul Yousuf, Abu M. Amirul, Islam H. Naif, Yasir C. K. M., Faizal Alam, Md. Zahangir Pirozzi, Domenico
author_facet	Karim, Ahasanul Yousuf, Abu M. Amirul, Islam H. Naif, Yasir C. K. M., Faizal Alam, Md. Zahangir Pirozzi, Domenico
author_sort	Karim, Ahasanul
title	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_short	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_full	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_fullStr	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_full_unstemmed	Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
title_sort	microbial lipid extraction from lipomyces starkeyi using irreversible electroporation
publisher	John Wiley and Sons Inc.
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/22932/ http://umpir.ump.edu.my/id/eprint/22932/ http://umpir.ump.edu.my/id/eprint/22932/ http://umpir.ump.edu.my/id/eprint/22932/1/Microbial%20lipid%20extraction%20from%20Lipomyces%20starkeyi%20using%20irreversible%20electroporation.pdf
first_indexed	2023-09-18T22:34:07Z
last_indexed	2023-09-18T22:34:07Z
_version_	1777416481517076480

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

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