Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane

Cyclodextrin glucanotransferase (CGTase) is a starch degrading enzyme to produce cyclodextrin (CD), which can encapsulate compounds to remove flavors and aroma. However, the use of the enzyme is often limited due to the instability of the enzyme resulting in low production of CD. Immobilization tech...

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Main Authors:	S., Suhaimi, Rohaida, Che Man, N., Jamil, Zatul Iffah, Mohd Arshad, Shalyda, Md Shaarani, Siti Kholijah, Abdul Mudalip, Siti Zubaidah, Sulaiman, Aizi Nor Mazila, Ramli
Format:	Conference or Workshop Item
Language:	English
Published:	IOP Publishing Ltd. 2018
Subjects:	Q Science (General) TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/22620/ http://umpir.ump.edu.my/id/eprint/22620/ http://umpir.ump.edu.my/id/eprint/22620/1/Stability%20and%20reusability%20of%20cyclodextrin%20glucanotransferase%20immobilized%20on%20hollow%20fiber%20membrane.pdf

id	ump-22620
recordtype	eprints
spelling	ump-226202018-12-14T03:07:20Z http://umpir.ump.edu.my/id/eprint/22620/ Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli Q Science (General) TP Chemical technology Cyclodextrin glucanotransferase (CGTase) is a starch degrading enzyme to produce cyclodextrin (CD), which can encapsulate compounds to remove flavors and aroma. However, the use of the enzyme is often limited due to the instability of the enzyme resulting in low production of CD. Immobilization technique has greatly improved the characteristics of the enzyme during the reaction. In this study, CGTase was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption method. The stability and reusability of the immobilized CGTase were studied and compared with free form of the CGTase. Thermal stability of the immobilized CGTase able to retain 50% of the initial residual activity at temperature up to 70°C. While there was an improvement in pH stability with a wider pH range from pH 5 to pH 8. The reusability of the immobilized CGTase was able to retain up to 40% of the initial CD production after repeatedly used for 10 cycles. Hence, the immobilization of CGTase shows a good physical and chemical resistance, which may be advantageous to be applied in industry. IOP Publishing Ltd. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22620/1/Stability%20and%20reusability%20of%20cyclodextrin%20glucanotransferase%20immobilized%20on%20hollow%20fiber%20membrane.pdf S., Suhaimi and Rohaida, Che Man and N., Jamil and Zatul Iffah, Mohd Arshad and Shalyda, Md Shaarani and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Aizi Nor Mazila, Ramli (2018) Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane. In: 4th International Conference on Agricultural and Biological Sciences (ABS 2018), 26-29 June 2018 , Hangzhou, Zhejiang Province, China. pp. 1-7., 185 (1). ISSN 1755-1307 http://iopscience.iop.org/article/10.1088/1755-1315/185/1/012002/meta
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) TP Chemical technology
spellingShingle	Q Science (General) TP Chemical technology S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
description	Cyclodextrin glucanotransferase (CGTase) is a starch degrading enzyme to produce cyclodextrin (CD), which can encapsulate compounds to remove flavors and aroma. However, the use of the enzyme is often limited due to the instability of the enzyme resulting in low production of CD. Immobilization technique has greatly improved the characteristics of the enzyme during the reaction. In this study, CGTase was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption method. The stability and reusability of the immobilized CGTase were studied and compared with free form of the CGTase. Thermal stability of the immobilized CGTase able to retain 50% of the initial residual activity at temperature up to 70°C. While there was an improvement in pH stability with a wider pH range from pH 5 to pH 8. The reusability of the immobilized CGTase was able to retain up to 40% of the initial CD production after repeatedly used for 10 cycles. Hence, the immobilization of CGTase shows a good physical and chemical resistance, which may be advantageous to be applied in industry.
format	Conference or Workshop Item
author	S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli
author_facet	S., Suhaimi Rohaida, Che Man N., Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Aizi Nor Mazila, Ramli
author_sort	S., Suhaimi
title	Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
title_short	Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
title_full	Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
title_fullStr	Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
title_full_unstemmed	Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
title_sort	stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane
publisher	IOP Publishing Ltd.
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/22620/ http://umpir.ump.edu.my/id/eprint/22620/ http://umpir.ump.edu.my/id/eprint/22620/1/Stability%20and%20reusability%20of%20cyclodextrin%20glucanotransferase%20immobilized%20on%20hollow%20fiber%20membrane.pdf
first_indexed	2023-09-18T22:33:47Z
last_indexed	2023-09-18T22:33:47Z
_version_	1777416459869224960

Stability and reusability of cyclodextrin glucanotransferase immobilized on hollow fiber membrane

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