Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products

The benefits of Eurycoma longifolia (Tongkat Ali, TA) for its aphrodisiac capabilities are well known and many products are marketed worldwide. Due to its popularity, the plant is being abused for promoting fake products. Therefore, there is a need for better testing of the markers required by autho...

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Main Authors:	Jaya Vejayan, Palliah, Aini Norhidayah, Mohamed, Amira Alia, Zulkifli, Yasmin Amira, Che Yahya, Norliana, Munir, M. M., Yusoff
Format:	Article
Language:	English English
Published:	ndian Academy of Sciences 2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/22572/ http://umpir.ump.edu.my/id/eprint/22572/ http://umpir.ump.edu.my/id/eprint/22572/1/Marker%20to%20authenticate%20Eurycoma%20longifolia%20%28Tongkat%20Ali%29%20containing%20aphrodisiac%20herbal%20products.pdf http://umpir.ump.edu.my/id/eprint/22572/7/Marker%20to%20authenticate%20Eurycoma%20longifolia%20%28Tongkat%20Ali%29%20containing%20aphrodisiac%20herbal%20products.pdf

id	ump-22572
recordtype	eprints
spelling	ump-225722018-12-03T04:26:54Z http://umpir.ump.edu.my/id/eprint/22572/ Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products Jaya Vejayan, Palliah Aini Norhidayah, Mohamed Amira Alia, Zulkifli Yasmin Amira, Che Yahya Norliana, Munir M. M., Yusoff Q Science (General) The benefits of Eurycoma longifolia (Tongkat Ali, TA) for its aphrodisiac capabilities are well known and many products are marketed worldwide. Due to its popularity, the plant is being abused for promoting fake products. Therefore, there is a need for better testing of the markers required by authorities and responsible manufacturers. A low-molecular-weight protein has been studied for developing it as a testing marker. Two dimensional electrophoresis (2DE) (four spots were observed) was used for positive detection of proteins in an aqueous extract of TA root and the pronounced separation of a Coomassie-stained spot, subsequently referred to as Marker A. Consecutive chromatographic separations of the aqueous extract of TA led to the isolation of pure protein from Marker A. When this marker was used to test 46 TA-based products randomly selected from markets worldwide, in 20 of them, the results were found to be comparable to those obtained using the organic eurycomanone marker. The ranking of products from the highest quantity to the lowest observed to be ordered differently if compared for both markers. This is an expected outcome because Marker A was measured for its protein content and eurycomanone for its organic molecule. Marker A detection using 2DE is shown to be a useful tool to test products supplemented with E. longifolia root. ndian Academy of Sciences 2018-09 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22572/1/Marker%20to%20authenticate%20Eurycoma%20longifolia%20%28Tongkat%20Ali%29%20containing%20aphrodisiac%20herbal%20products.pdf pdf en http://umpir.ump.edu.my/id/eprint/22572/7/Marker%20to%20authenticate%20Eurycoma%20longifolia%20%28Tongkat%20Ali%29%20containing%20aphrodisiac%20herbal%20products.pdf Jaya Vejayan, Palliah and Aini Norhidayah, Mohamed and Amira Alia, Zulkifli and Yasmin Amira, Che Yahya and Norliana, Munir and M. M., Yusoff (2018) Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products. Current Science, 115 (5). pp. 886-894. ISSN 0011-3891 http://www.currentscience.ac.in/Volumes/115/05/0886.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Jaya Vejayan, Palliah Aini Norhidayah, Mohamed Amira Alia, Zulkifli Yasmin Amira, Che Yahya Norliana, Munir M. M., Yusoff Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products
description	The benefits of Eurycoma longifolia (Tongkat Ali, TA) for its aphrodisiac capabilities are well known and many products are marketed worldwide. Due to its popularity, the plant is being abused for promoting fake products. Therefore, there is a need for better testing of the markers required by authorities and responsible manufacturers. A low-molecular-weight protein has been studied for developing it as a testing marker. Two dimensional electrophoresis (2DE) (four spots were observed) was used for positive detection of proteins in an aqueous extract of TA root and the pronounced separation of a Coomassie-stained spot, subsequently referred to as Marker A. Consecutive chromatographic separations of the aqueous extract of TA led to the isolation of pure protein from Marker A. When this marker was used to test 46 TA-based products randomly selected from markets worldwide, in 20 of them, the results were found to be comparable to those obtained using the organic eurycomanone marker. The ranking of products from the highest quantity to the lowest observed to be ordered differently if compared for both markers. This is an expected outcome because Marker A was measured for its protein content and eurycomanone for its organic molecule. Marker A detection using 2DE is shown to be a useful tool to test products supplemented with E. longifolia root.
format	Article
author	Jaya Vejayan, Palliah Aini Norhidayah, Mohamed Amira Alia, Zulkifli Yasmin Amira, Che Yahya Norliana, Munir M. M., Yusoff
author_facet	Jaya Vejayan, Palliah Aini Norhidayah, Mohamed Amira Alia, Zulkifli Yasmin Amira, Che Yahya Norliana, Munir M. M., Yusoff
author_sort	Jaya Vejayan, Palliah
title	Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products
title_short	Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products
title_full	Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products
title_fullStr	Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products
title_full_unstemmed	Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products
title_sort	marker to authenticate eurycoma longifolia (tongkat ali) containing aphrodisiac herbal products
publisher	ndian Academy of Sciences
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/22572/ http://umpir.ump.edu.my/id/eprint/22572/ http://umpir.ump.edu.my/id/eprint/22572/1/Marker%20to%20authenticate%20Eurycoma%20longifolia%20%28Tongkat%20Ali%29%20containing%20aphrodisiac%20herbal%20products.pdf http://umpir.ump.edu.my/id/eprint/22572/7/Marker%20to%20authenticate%20Eurycoma%20longifolia%20%28Tongkat%20Ali%29%20containing%20aphrodisiac%20herbal%20products.pdf
first_indexed	2023-09-18T22:33:41Z
last_indexed	2023-09-18T22:33:41Z
_version_	1777416453329256448

Marker to authenticate Eurycoma longifolia (Tongkat Ali) containing aphrodisiac herbal products

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