Isolation of solubilizing microorganisms from landfill soil

characterization and screening of cellulolytic and xylanolytic microorganisms from landfill soil and quantification of their enzymatic activity. Sample was collected from landfill soil of landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Isolation of the solubilizing microorganism was conducted...

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Main Author:	Siti Nor Amira, Rosli
Format:	Undergraduates Project Papers
Language:	English English English English
Published:	2017
Subjects:	QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/22494/ http://umpir.ump.edu.my/id/eprint/22494/ http://umpir.ump.edu.my/id/eprint/22494/1/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/22494/2/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/22494/3/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/22494/4/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20References.pdf

id	ump-22494
recordtype	eprints
spelling	ump-224942018-12-11T01:42:00Z http://umpir.ump.edu.my/id/eprint/22494/ Isolation of solubilizing microorganisms from landfill soil Siti Nor Amira, Rosli QR Microbiology characterization and screening of cellulolytic and xylanolytic microorganisms from landfill soil and quantification of their enzymatic activity. Sample was collected from landfill soil of landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Isolation of the solubilizing microorganism was conducted via standard serial dilution and spread plate method using nutrient agar medium and these microorganisms were further characterized by morphological characteristics and Gram-staining. Screening tests demonstrated the ability of microorganism to produce cellulase and xylanase enzyme via Gram’s iodine-staining. Finally, selected isolates were subjected to enzyme activity quantification such as total cellulase, endoglucanase, exoglucanase and xylanase assay analysis. A total of 58 different isolates were isolated from landfill soil which Gram-positive bacilli were the dominant microorganism. Out of 58 isolates, 29 isolates were selected for further testing. After Gram’s iodine staining, there were 23 cellulolytic microorganisms and 26 xylanolytic microorganisms which showed clear zones on agar plate containing carboxymethyl cellulose and xylan, respectively. Two isolates out of 23 cellulolytic microorganisms and three isolates out of 26 xylanolytic microorganisms with ratio of clear zone (D/d) more than 3.3 were furthered tested for quantification of enzyme activity. Cellulolytic microorganism M12 had the highest endoglucanase activity at 1.7621±0.0054 U/mL while cellulolytic microorganism M28 had the highest total cellulase activity and exoglucanase activity at 1.6086±0.0007 U/mL and 1.8299±0.0704 U/mL, respectively. Meanwhile, xylanolytic microorganism M15 had the highest xylanase activity at 8235±0.1715 U/mL. In conclusion, the 58 microorganisms were isolated which initiated research on industrially important microorganism from landfill soil and these isolates may be vital source for the discovery of industrial useful enzymes especially in municipal solid waste degradation. 2017-01 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22494/1/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/22494/2/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/22494/3/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/22494/4/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20References.pdf Siti Nor Amira, Rosli (2017) Isolation of solubilizing microorganisms from landfill soil. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:102804&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Siti Nor Amira, Rosli Isolation of solubilizing microorganisms from landfill soil
description	characterization and screening of cellulolytic and xylanolytic microorganisms from landfill soil and quantification of their enzymatic activity. Sample was collected from landfill soil of landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Isolation of the solubilizing microorganism was conducted via standard serial dilution and spread plate method using nutrient agar medium and these microorganisms were further characterized by morphological characteristics and Gram-staining. Screening tests demonstrated the ability of microorganism to produce cellulase and xylanase enzyme via Gram’s iodine-staining. Finally, selected isolates were subjected to enzyme activity quantification such as total cellulase, endoglucanase, exoglucanase and xylanase assay analysis. A total of 58 different isolates were isolated from landfill soil which Gram-positive bacilli were the dominant microorganism. Out of 58 isolates, 29 isolates were selected for further testing. After Gram’s iodine staining, there were 23 cellulolytic microorganisms and 26 xylanolytic microorganisms which showed clear zones on agar plate containing carboxymethyl cellulose and xylan, respectively. Two isolates out of 23 cellulolytic microorganisms and three isolates out of 26 xylanolytic microorganisms with ratio of clear zone (D/d) more than 3.3 were furthered tested for quantification of enzyme activity. Cellulolytic microorganism M12 had the highest endoglucanase activity at 1.7621±0.0054 U/mL while cellulolytic microorganism M28 had the highest total cellulase activity and exoglucanase activity at 1.6086±0.0007 U/mL and 1.8299±0.0704 U/mL, respectively. Meanwhile, xylanolytic microorganism M15 had the highest xylanase activity at 8235±0.1715 U/mL. In conclusion, the 58 microorganisms were isolated which initiated research on industrially important microorganism from landfill soil and these isolates may be vital source for the discovery of industrial useful enzymes especially in municipal solid waste degradation.
format	Undergraduates Project Papers
author	Siti Nor Amira, Rosli
author_facet	Siti Nor Amira, Rosli
author_sort	Siti Nor Amira, Rosli
title	Isolation of solubilizing microorganisms from landfill soil
title_short	Isolation of solubilizing microorganisms from landfill soil
title_full	Isolation of solubilizing microorganisms from landfill soil
title_fullStr	Isolation of solubilizing microorganisms from landfill soil
title_full_unstemmed	Isolation of solubilizing microorganisms from landfill soil
title_sort	isolation of solubilizing microorganisms from landfill soil
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/22494/ http://umpir.ump.edu.my/id/eprint/22494/ http://umpir.ump.edu.my/id/eprint/22494/1/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/22494/2/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/22494/3/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/22494/4/Isolation%20of%20solubilizing%20microorganisms%20from%20landfill%20soil%20-%20References.pdf
first_indexed	2023-09-18T22:33:31Z
last_indexed	2023-09-18T22:33:31Z
_version_	1777416443068940288

Isolation of solubilizing microorganisms from landfill soil

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