Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)

Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on cha...

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Main Author:	Nur 'Izzaty Hazera, Nazar
Format:	Undergraduates Project Papers
Language:	English English English English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/22410/ http://umpir.ump.edu.my/id/eprint/22410/ http://umpir.ump.edu.my/id/eprint/22410/1/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/22410/2/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/22410/3/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/22410/4/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20References.pdf

id	ump-22410
recordtype	eprints
spelling	ump-224102018-10-25T02:08:00Z http://umpir.ump.edu.my/id/eprint/22410/ Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE) Nur 'Izzaty Hazera, Nazar TP Chemical technology Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses. 2016-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22410/1/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/22410/2/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/22410/3/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/22410/4/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20References.pdf Nur 'Izzaty Hazera, Nazar (2016) Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:102806&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur 'Izzaty Hazera, Nazar Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
description	Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses.
format	Undergraduates Project Papers
author	Nur 'Izzaty Hazera, Nazar
author_facet	Nur 'Izzaty Hazera, Nazar
author_sort	Nur 'Izzaty Hazera, Nazar
title	Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_short	Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_full	Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_fullStr	Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_full_unstemmed	Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_sort	effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (cgtase)
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/22410/ http://umpir.ump.edu.my/id/eprint/22410/ http://umpir.ump.edu.my/id/eprint/22410/1/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Table%20of%20contents.pdf http://umpir.ump.edu.my/id/eprint/22410/2/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Abstract.pdf http://umpir.ump.edu.my/id/eprint/22410/3/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Chapter%201.pdf http://umpir.ump.edu.my/id/eprint/22410/4/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20References.pdf
first_indexed	2023-09-18T22:33:20Z
last_indexed	2023-09-18T22:33:20Z
_version_	1777416432121806848

Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)

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