Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro

Italian ryegrass is one of main feed for livestock animals/birds. It has potential antioxidant metabolites that can improve their health and protect them against various infectious diseases. In this work, we studied synthesis of silver nanoparticles assisted by forage crop Lolium multiflorum as a gr...

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Main Authors:	Lee, Kyung Dong, Kuppusamy, Palaniselvam, Kim, Da Hye, Natanamurugaraj, Govindan, Gaanty Pragas, Maniam, Choi, Ki Choon
Format:	Article
Language:	English
Published:	Springer 2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/22276/ http://umpir.ump.edu.my/id/eprint/22276/ http://umpir.ump.edu.my/id/eprint/22276/ http://umpir.ump.edu.my/id/eprint/22276/1/Forage%20Crop%20Lolium%20multiflorum%20Assisted%20Synthesis%20of%20AgNPs%20and%20their%20bioactivities%20against%20poultry%20pathogenic%20bacteria%20in%20in%20vitro.pdf

id	ump-22276
recordtype	eprints
spelling	ump-222762018-10-11T07:48:52Z http://umpir.ump.edu.my/id/eprint/22276/ Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro Lee, Kyung Dong Kuppusamy, Palaniselvam Kim, Da Hye Natanamurugaraj, Govindan Gaanty Pragas, Maniam Choi, Ki Choon Q Science (General) Italian ryegrass is one of main feed for livestock animals/birds. It has potential antioxidant metabolites that can improve their health and protect them against various infectious diseases. In this work, we studied synthesis of silver nanoparticles assisted by forage crop Lolium multiflorum as a green synthesis way. Potential antibacterial efficacy of these synthesized nanosized silver nanoparticles against poultry pathogenic bacteria was then studied. Aqueous extract of IRG was used as reducing agent for bio-reduction of silver salt to convert Ag+ to Ag0 metallic nano-silver. Size, shape, metallic composition, functional group, and crystalline nature of these synthesized silver nanoparticles were then characterized using UV–Vis spectrophotometer, FESEM, EDX, FT-IT, and XRD, respectively. In addition, antibacterial effects of these synthesized AgNPs against poultry pathogenic bacteria were evaluated by agar well diffusion method. UV–Vis spectra showed strong absorption peak of 440–450 nm with differ reaction time ranging from 30 min to 24 h. FESEM measurements revealed particles sizes of around 20–100 nm, majority of which were spherical in shape while a few were irregular. These biosynthesized silver nanoparticles using IRG extract exhibited strong antibacterial activities against poultry pathogenic microorganisms, including Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, and Bacillus subtilis. Overall results confirmed that IRG plant extract possessed potential bioactive compounds for converting silver ions into nanosized silver at room temperature without needing any external chemical for redox reaction. In addition, such synthesized AgNPs showed strong antibacterial activities against pathogenic bacteria responsible for infectious diseases in poultry. Springer 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22276/1/Forage%20Crop%20Lolium%20multiflorum%20Assisted%20Synthesis%20of%20AgNPs%20and%20their%20bioactivities%20against%20poultry%20pathogenic%20bacteria%20in%20in%20vitro.pdf Lee, Kyung Dong and Kuppusamy, Palaniselvam and Kim, Da Hye and Natanamurugaraj, Govindan and Gaanty Pragas, Maniam and Choi, Ki Choon (2018) Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro. Indian Journal of Microbiology, 58 (4). pp. 507-514. ISSN 0973-7715 https://doi.org/10.1007/s12088-018-0755-8 doi: 10.1007/s12088-018-0755-8
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Lee, Kyung Dong Kuppusamy, Palaniselvam Kim, Da Hye Natanamurugaraj, Govindan Gaanty Pragas, Maniam Choi, Ki Choon Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
description	Italian ryegrass is one of main feed for livestock animals/birds. It has potential antioxidant metabolites that can improve their health and protect them against various infectious diseases. In this work, we studied synthesis of silver nanoparticles assisted by forage crop Lolium multiflorum as a green synthesis way. Potential antibacterial efficacy of these synthesized nanosized silver nanoparticles against poultry pathogenic bacteria was then studied. Aqueous extract of IRG was used as reducing agent for bio-reduction of silver salt to convert Ag+ to Ag0 metallic nano-silver. Size, shape, metallic composition, functional group, and crystalline nature of these synthesized silver nanoparticles were then characterized using UV–Vis spectrophotometer, FESEM, EDX, FT-IT, and XRD, respectively. In addition, antibacterial effects of these synthesized AgNPs against poultry pathogenic bacteria were evaluated by agar well diffusion method. UV–Vis spectra showed strong absorption peak of 440–450 nm with differ reaction time ranging from 30 min to 24 h. FESEM measurements revealed particles sizes of around 20–100 nm, majority of which were spherical in shape while a few were irregular. These biosynthesized silver nanoparticles using IRG extract exhibited strong antibacterial activities against poultry pathogenic microorganisms, including Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, and Bacillus subtilis. Overall results confirmed that IRG plant extract possessed potential bioactive compounds for converting silver ions into nanosized silver at room temperature without needing any external chemical for redox reaction. In addition, such synthesized AgNPs showed strong antibacterial activities against pathogenic bacteria responsible for infectious diseases in poultry.
format	Article
author	Lee, Kyung Dong Kuppusamy, Palaniselvam Kim, Da Hye Natanamurugaraj, Govindan Gaanty Pragas, Maniam Choi, Ki Choon
author_facet	Lee, Kyung Dong Kuppusamy, Palaniselvam Kim, Da Hye Natanamurugaraj, Govindan Gaanty Pragas, Maniam Choi, Ki Choon
author_sort	Lee, Kyung Dong
title	Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_short	Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_full	Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_fullStr	Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_full_unstemmed	Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_sort	forage crop lolium multiflorum assisted synthesis of agnps and their bioactivities against poultry pathogenic bacteria in in vitro
publisher	Springer
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/22276/ http://umpir.ump.edu.my/id/eprint/22276/ http://umpir.ump.edu.my/id/eprint/22276/ http://umpir.ump.edu.my/id/eprint/22276/1/Forage%20Crop%20Lolium%20multiflorum%20Assisted%20Synthesis%20of%20AgNPs%20and%20their%20bioactivities%20against%20poultry%20pathogenic%20bacteria%20in%20in%20vitro.pdf
first_indexed	2023-09-18T22:33:04Z
last_indexed	2023-09-18T22:33:04Z
_version_	1777416415237636096

Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro

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