Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool

In this study, ethanolic microwave-assisted extraction of total phenolic content (TFC), total flavonoid content (TFC) and antioxidant from Vernonia amygdalina leaf was investigated using response surface methodology. Four extraction variables, including irradiation time (1‒5 min), microwave power le...

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Main Authors:	Alara, Oluwaseun Ruth, Nour, A. H., Olalere, Olusegun Abayomi
Format:	Article
Language:	English
Published:	Springer International Publishing 2018
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/21926/ http://umpir.ump.edu.my/id/eprint/21926/ http://umpir.ump.edu.my/id/eprint/21926/ http://umpir.ump.edu.my/id/eprint/21926/1/Ethanolic%20extraction%20of%20bioactive%20compounds%20from%20Vernonia1.pdf

id	ump-21926
recordtype	eprints
spelling	ump-219262019-09-10T04:02:21Z http://umpir.ump.edu.my/id/eprint/21926/ Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool Alara, Oluwaseun Ruth Nour, A. H. Olalere, Olusegun Abayomi TP Chemical technology In this study, ethanolic microwave-assisted extraction of total phenolic content (TFC), total flavonoid content (TFC) and antioxidant from Vernonia amygdalina leaf was investigated using response surface methodology. Four extraction variables, including irradiation time (1‒5 min), microwave power level (500‒700 W), feed-to-solvent ratio (1:8‒1:12 g/ml), and ethanol concentration (60‒80%) were optimized to obtain optimal yields. The analysis of variance results showed that ethanol concentration, microwave power level and irradiation time mostly affected the TFC, TPC and antioxidant activities significantly (p < 0.05), whereas the feed-to-solvent ratio was insignificant. The validated optimal yields of TPC (113.76 mg GAE/g d.w.), TFC (94.08 mg QE/g d.w.), DPPH (97.98%), and ABTS (99.34%) were obtained at extraction conditions, viz, 4 min of irradiation time, 558 W of microwave power, feed-to-solvent ratio of 1:10 g/ml, and ethanol concentration of 76%. In addition, the phytochemical profiling of the extract at optimal conditions confirmed the presence of phenolic and flavonoid compounds. Springer International Publishing 2018-02-31 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21926/1/Ethanolic%20extraction%20of%20bioactive%20compounds%20from%20Vernonia1.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Olalere, Olusegun Abayomi (2018) Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool. Journal of Food Measurement and Characterization, 12 (2). pp. 1107-1122. ISSN 2193-4126 (Print); 2193-4134 (Online) https://doi.org/10.1007/s11694-018-9726-3 https://doi.org/10.1007/s11694-018-9726-3
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Alara, Oluwaseun Ruth Nour, A. H. Olalere, Olusegun Abayomi Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool
description	In this study, ethanolic microwave-assisted extraction of total phenolic content (TFC), total flavonoid content (TFC) and antioxidant from Vernonia amygdalina leaf was investigated using response surface methodology. Four extraction variables, including irradiation time (1‒5 min), microwave power level (500‒700 W), feed-to-solvent ratio (1:8‒1:12 g/ml), and ethanol concentration (60‒80%) were optimized to obtain optimal yields. The analysis of variance results showed that ethanol concentration, microwave power level and irradiation time mostly affected the TFC, TPC and antioxidant activities significantly (p < 0.05), whereas the feed-to-solvent ratio was insignificant. The validated optimal yields of TPC (113.76 mg GAE/g d.w.), TFC (94.08 mg QE/g d.w.), DPPH (97.98%), and ABTS (99.34%) were obtained at extraction conditions, viz, 4 min of irradiation time, 558 W of microwave power, feed-to-solvent ratio of 1:10 g/ml, and ethanol concentration of 76%. In addition, the phytochemical profiling of the extract at optimal conditions confirmed the presence of phenolic and flavonoid compounds.
format	Article
author	Alara, Oluwaseun Ruth Nour, A. H. Olalere, Olusegun Abayomi
author_facet	Alara, Oluwaseun Ruth Nour, A. H. Olalere, Olusegun Abayomi
author_sort	Alara, Oluwaseun Ruth
title	Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool
title_short	Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool
title_full	Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool
title_fullStr	Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool
title_full_unstemmed	Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool
title_sort	ethanolic extraction of bioactive compounds from vernonia amygdalina leaf using response surface methodology as an optimization tool
publisher	Springer International Publishing
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/21926/ http://umpir.ump.edu.my/id/eprint/21926/ http://umpir.ump.edu.my/id/eprint/21926/ http://umpir.ump.edu.my/id/eprint/21926/1/Ethanolic%20extraction%20of%20bioactive%20compounds%20from%20Vernonia1.pdf
first_indexed	2023-09-18T22:32:24Z
last_indexed	2023-09-18T22:32:24Z
_version_	1777416372640284672

Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool

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