Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia

The extracts from Vernonia amygdalina leaves dried using different modes of drying were examined for their antioxidant activities. The component identification was carried out on extract with the highest antioxidant property using GC–MS analysis. Using DPPH, ABTS+ and H2O2 radical scavenging assays...

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Main Authors:	Alara, Oluwaseun Ruth, Nour, A. H., Siti Kholijah, Abdul Mudalip, Olalere, Olusegun Abayomi
Format:	Article
Language:	English
Published:	King Saud University 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/21919/ http://umpir.ump.edu.my/id/eprint/21919/ http://umpir.ump.edu.my/id/eprint/21919/ http://umpir.ump.edu.my/id/eprint/21919/1/DJ1.pdf

id	ump-21919
recordtype	eprints
spelling	ump-219192019-09-10T04:08:16Z http://umpir.ump.edu.my/id/eprint/21919/ Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi TP Chemical technology The extracts from Vernonia amygdalina leaves dried using different modes of drying were examined for their antioxidant activities. The component identification was carried out on extract with the highest antioxidant property using GC–MS analysis. Using DPPH, ABTS+ and H2O2 radical scavenging assays, the shade, the sun, oven at 40, 50, and 60 °C dried sample extracts were screened. The obtained scavenging activities were in the order: shade drying > sun drying > oven drying at 40 °C > oven drying at 50 °C > oven drying at 60 °C. The extract from shade-dried sample exhibited the highest activity by scavenging 63.21–92.25% of DPPH, 75.32–93.15% of ABTS+ , and 82.53–95.78% of H2O2 radicals, which were higher than those of ascorbic and gallic acid scavenging activities. In addition, the effects of drying methods and extract concentrations on the antioxidant activity of V. amygdalina leaf were significant (p < 0.05). A total number of 11 major compounds were identified from the shade-dried extract viz: phytol (43.69%), (z,z,z)-methyl ester-9,12,15-octadecatrienoic acid (17.60%), 2-methyl-3-hexanol (6.83%), ethyl ester-9,12-octadecadienoic acid (6.07%), ethyl ester linoleic acid (5.19%), ethyl ester hexadecanoic acid (5.06), heneicosane (4.15%), heptacosane (3.25%), and others are less than 3%. Therefore, it can be deduced that an increase in drying temperature may deteriorate the antioxidant activity of Vernonia amygdalina leaf. King Saud University 2017-05-29 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21919/1/DJ1.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Siti Kholijah, Abdul Mudalip and Olalere, Olusegun Abayomi (2017) Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia. Journal of King Saud University - Science. pp. 1-5. ISSN 1018-3647 (In Press) https://doi.org/10.1016/j.jksus.2017.05.018 http://dx.doi.org/10.1016/j.jksus.2017.05.018
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
description	The extracts from Vernonia amygdalina leaves dried using different modes of drying were examined for their antioxidant activities. The component identification was carried out on extract with the highest antioxidant property using GC–MS analysis. Using DPPH, ABTS+ and H2O2 radical scavenging assays, the shade, the sun, oven at 40, 50, and 60 °C dried sample extracts were screened. The obtained scavenging activities were in the order: shade drying > sun drying > oven drying at 40 °C > oven drying at 50 °C > oven drying at 60 °C. The extract from shade-dried sample exhibited the highest activity by scavenging 63.21–92.25% of DPPH, 75.32–93.15% of ABTS+ , and 82.53–95.78% of H2O2 radicals, which were higher than those of ascorbic and gallic acid scavenging activities. In addition, the effects of drying methods and extract concentrations on the antioxidant activity of V. amygdalina leaf were significant (p < 0.05). A total number of 11 major compounds were identified from the shade-dried extract viz: phytol (43.69%), (z,z,z)-methyl ester-9,12,15-octadecatrienoic acid (17.60%), 2-methyl-3-hexanol (6.83%), ethyl ester-9,12-octadecadienoic acid (6.07%), ethyl ester linoleic acid (5.19%), ethyl ester hexadecanoic acid (5.06), heneicosane (4.15%), heptacosane (3.25%), and others are less than 3%. Therefore, it can be deduced that an increase in drying temperature may deteriorate the antioxidant activity of Vernonia amygdalina leaf.
format	Article
author	Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi
author_facet	Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi
author_sort	Alara, Oluwaseun Ruth
title	Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_short	Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_full	Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_fullStr	Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_full_unstemmed	Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia
title_sort	effect of drying methods on the free radicals scavenging activity of vernonia amygdalina growing in malaysia
publisher	King Saud University
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/21919/ http://umpir.ump.edu.my/id/eprint/21919/ http://umpir.ump.edu.my/id/eprint/21919/ http://umpir.ump.edu.my/id/eprint/21919/1/DJ1.pdf
first_indexed	2023-09-18T22:32:23Z
last_indexed	2023-09-18T22:32:23Z
_version_	1777416371620020224

Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia

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