Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata

Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata

Oil palm frond (OPF) juice has been known as a good source of sugars to replace commercial sugars to produce methyl 3- (3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The aim of this study was to investigate the effect of temperature (°C), initial pH medium, agitation speed (rpm) and concentration o...

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Main Authors: Nang Nor Azimah, Long Nadzri, Mior Ahmad Khushairi, Mohd Zahari, Saiful Nizam, Tajuddin, Che Mohd Aizal, Che Mohd
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 2018
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/21772/
http://umpir.ump.edu.my/id/eprint/21772/
http://umpir.ump.edu.my/id/eprint/21772/1/Full%20Factorial%20Design%20for%20Production%20of%20Methyl%203.pdf
id ump-21772
recordtype eprints
spelling ump-217722018-07-24T08:18:38Z http://umpir.ump.edu.my/id/eprint/21772/ Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata Nang Nor Azimah, Long Nadzri Mior Ahmad Khushairi, Mohd Zahari Saiful Nizam, Tajuddin Che Mohd Aizal, Che Mohd TP Chemical technology Oil palm frond (OPF) juice has been known as a good source of sugars to replace commercial sugars to produce methyl 3- (3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The aim of this study was to investigate the effect of temperature (°C), initial pH medium, agitation speed (rpm) and concentration of glucose (g/L) on the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate from OPF juice by using Ceratocystis fimbriata (C. fimbriata). The design of experiment (DOE) method was used to screen the best parameters affecting the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The Gas Chromatography-Mass Spectroscopy (GC-MS) with Solid Phase Micro Extraction (SPME) was used to analyze and separate the peak of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. Based on the GC-MS analysis, results showed that the most favorable condition for methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate production was achieved at an initial pH medium (8), agitation speed (100 rpm), temperature (25°C) and 30 g/L of glucose in OPF juice. Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate were produced when the retention time was 32.80 min, the relative peak area was 0.24 % of chromatogram area. This result showed the great potential usage of OPF juice as a substrate to produce methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate by C. fimbriata. Malaysian Society of Applied Biology 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21772/1/Full%20Factorial%20Design%20for%20Production%20of%20Methyl%203.pdf Nang Nor Azimah, Long Nadzri and Mior Ahmad Khushairi, Mohd Zahari and Saiful Nizam, Tajuddin and Che Mohd Aizal, Che Mohd (2018) Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata. Malaysian Applied Biology, 47 (2). pp. 9-13. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=745&catid=59:current-view&Itemid=56
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nang Nor Azimah, Long Nadzri
Mior Ahmad Khushairi, Mohd Zahari
Saiful Nizam, Tajuddin
Che Mohd Aizal, Che Mohd
Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata
description Oil palm frond (OPF) juice has been known as a good source of sugars to replace commercial sugars to produce methyl 3- (3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The aim of this study was to investigate the effect of temperature (°C), initial pH medium, agitation speed (rpm) and concentration of glucose (g/L) on the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate from OPF juice by using Ceratocystis fimbriata (C. fimbriata). The design of experiment (DOE) method was used to screen the best parameters affecting the production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. The Gas Chromatography-Mass Spectroscopy (GC-MS) with Solid Phase Micro Extraction (SPME) was used to analyze and separate the peak of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate. Based on the GC-MS analysis, results showed that the most favorable condition for methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate production was achieved at an initial pH medium (8), agitation speed (100 rpm), temperature (25°C) and 30 g/L of glucose in OPF juice. Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate were produced when the retention time was 32.80 min, the relative peak area was 0.24 % of chromatogram area. This result showed the great potential usage of OPF juice as a substrate to produce methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate by C. fimbriata.
format Article
author Nang Nor Azimah, Long Nadzri
Mior Ahmad Khushairi, Mohd Zahari
Saiful Nizam, Tajuddin
Che Mohd Aizal, Che Mohd
author_facet Nang Nor Azimah, Long Nadzri
Mior Ahmad Khushairi, Mohd Zahari
Saiful Nizam, Tajuddin
Che Mohd Aizal, Che Mohd
author_sort Nang Nor Azimah, Long Nadzri
title Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata
title_short Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata
title_full Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata
title_fullStr Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata
title_full_unstemmed Full Factorial Design for Production of Methyl 3-(3,5-Di-Tert-Butyl-4-Hydroxyphenyl) Propionate Using Oil Palm Frond Juice as a Sole Substrate by Ceratocystis fimbriata
title_sort full factorial design for production of methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate using oil palm frond juice as a sole substrate by ceratocystis fimbriata
publisher Malaysian Society of Applied Biology
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/21772/
http://umpir.ump.edu.my/id/eprint/21772/
http://umpir.ump.edu.my/id/eprint/21772/1/Full%20Factorial%20Design%20for%20Production%20of%20Methyl%203.pdf
first_indexed 2023-09-18T22:32:05Z
last_indexed 2023-09-18T22:32:05Z
_version_ 1777416353460781056

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