Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed

The traditional ways of protein extraction is disadvantageous from both economic and environment perspective. In this study, ultrasonic assisted extraction, UAE technique was used for the first time for the optimization process of extracting proteins from Eurycoma longifolia roots. The experiments d...

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Main Authors:	Elhag, Husam Eldin Elhag Abugabr, Nailaa, Aishath, Nour, A. H., Azilah, Ajit, Ahmad Ziad, Sulaiman, Badhrulhisham, Abdul Aziz
Format:	Article
Language:	English
Published:	King Saud University 2018
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/21769/ http://umpir.ump.edu.my/id/eprint/21769/ http://umpir.ump.edu.my/id/eprint/21769/1/Optimization%20of%20protein%20yields%20by%20ultrasound%20assisted-INPRESS.pdf

id	ump-21769
recordtype	eprints
spelling	ump-217692018-07-24T07:20:13Z http://umpir.ump.edu.my/id/eprint/21769/ Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed Elhag, Husam Eldin Elhag Abugabr Nailaa, Aishath Nour, A. H. Azilah, Ajit Ahmad Ziad, Sulaiman Badhrulhisham, Abdul Aziz TP Chemical technology The traditional ways of protein extraction is disadvantageous from both economic and environment perspective. In this study, ultrasonic assisted extraction, UAE technique was used for the first time for the optimization process of extracting proteins from Eurycoma longifolia roots. The experiments demonstrated the successful effect of applying eccentric agitation with UAE to provide a combination of agitation and sonication to extract the proteins in short extraction times. Central composite design (CCD) was used to optimize the Ultrasonic-assisted extraction of proteins from roots of Eurycoma longifolia by water. Response surface methodology (RSM) was employed to investigate the effects of five independent variables; particle sizes (A), extraction temperatures (B), agitation speeds (C), amplitude (D) and duty cycle (E). The second enhanced model (Y2) prevailed to be more efficient in protein recovery with optimum conditions of A: 0.022 ± 0.022 mm, B: 49 °C, C: 1314 rpm, D: 9 W and E: 63% UI: 2.94 W·cm−2 was 9.543 ± 0. 946%. High agitation speed didn’t only affect the solvent properties, the diffusion of the solutes from the particles, cooling the extraction temperature, but influenced the distribution of cavitation bubbles and their impact on the solid particles. UAE was found to minimize the extraction of the same amount by conventional extraction 7-fold times. The results obtained in this study have exposed the capability of ultrasonic assisted extraction, UAE technology in extraction of protein from E. longifolia roots. Further works are nevertheless required to provide deeper understanding of the mechanisms involved to facilitate the development of optimum system applicable to the industry. King Saud University 2018 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/21769/1/Optimization%20of%20protein%20yields%20by%20ultrasound%20assisted-INPRESS.pdf Elhag, Husam Eldin Elhag Abugabr and Nailaa, Aishath and Nour, A. H. and Azilah, Ajit and Ahmad Ziad, Sulaiman and Badhrulhisham, Abdul Aziz (2018) Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed. Journal of King Saud University - Science. pp. 1-10. ISSN 1018-3647 (In Press) https://doi.org/10.1016/j.jksus.2018.05.011
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Elhag, Husam Eldin Elhag Abugabr Nailaa, Aishath Nour, A. H. Azilah, Ajit Ahmad Ziad, Sulaiman Badhrulhisham, Abdul Aziz Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed
description	The traditional ways of protein extraction is disadvantageous from both economic and environment perspective. In this study, ultrasonic assisted extraction, UAE technique was used for the first time for the optimization process of extracting proteins from Eurycoma longifolia roots. The experiments demonstrated the successful effect of applying eccentric agitation with UAE to provide a combination of agitation and sonication to extract the proteins in short extraction times. Central composite design (CCD) was used to optimize the Ultrasonic-assisted extraction of proteins from roots of Eurycoma longifolia by water. Response surface methodology (RSM) was employed to investigate the effects of five independent variables; particle sizes (A), extraction temperatures (B), agitation speeds (C), amplitude (D) and duty cycle (E). The second enhanced model (Y2) prevailed to be more efficient in protein recovery with optimum conditions of A: 0.022 ± 0.022 mm, B: 49 °C, C: 1314 rpm, D: 9 W and E: 63% UI: 2.94 W·cm−2 was 9.543 ± 0. 946%. High agitation speed didn’t only affect the solvent properties, the diffusion of the solutes from the particles, cooling the extraction temperature, but influenced the distribution of cavitation bubbles and their impact on the solid particles. UAE was found to minimize the extraction of the same amount by conventional extraction 7-fold times. The results obtained in this study have exposed the capability of ultrasonic assisted extraction, UAE technology in extraction of protein from E. longifolia roots. Further works are nevertheless required to provide deeper understanding of the mechanisms involved to facilitate the development of optimum system applicable to the industry.
format	Article
author	Elhag, Husam Eldin Elhag Abugabr Nailaa, Aishath Nour, A. H. Azilah, Ajit Ahmad Ziad, Sulaiman Badhrulhisham, Abdul Aziz
author_facet	Elhag, Husam Eldin Elhag Abugabr Nailaa, Aishath Nour, A. H. Azilah, Ajit Ahmad Ziad, Sulaiman Badhrulhisham, Abdul Aziz
author_sort	Elhag, Husam Eldin Elhag Abugabr
title	Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed
title_short	Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed
title_full	Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed
title_fullStr	Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed
title_full_unstemmed	Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed
title_sort	optimization of protein yields by ultrasound assisted extraction from eurycoma longifolia roots and effect of agitation speed
publisher	King Saud University
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/21769/ http://umpir.ump.edu.my/id/eprint/21769/ http://umpir.ump.edu.my/id/eprint/21769/1/Optimization%20of%20protein%20yields%20by%20ultrasound%20assisted-INPRESS.pdf
first_indexed	2023-09-18T22:32:05Z
last_indexed	2023-09-18T22:32:05Z
_version_	1777416353043447808

Optimization of protein yields by ultrasound assisted extraction from Eurycoma longifolia roots and effect of agitation speed

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