Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent...

Full description

Bibliographic Details
Main Authors:	Nurhusna, Samsudin, Yumi Zuhanis Has-Yun, Hashim, Mohd Azmir, Arifin, Maizirwan, Mel, Hamzah, Mohd. Salleh, Norshariza, Nordin, Sopyan, I., Dzun Noraini, Jimat
Format:	Article
Language:	English
Published:	Penerbit UTM Press 2017
Subjects:	T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/1/Statistical%20optimization%20of%20gelatin%20immobilisation.pdf

id	ump-21739
recordtype	eprints
spelling	ump-217392018-09-18T07:58:50Z http://umpir.ump.edu.my/id/eprint/21739/ Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility Nurhusna, Samsudin Yumi Zuhanis Has-Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, I. Dzun Noraini, Jimat T Technology (General) Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. Penerbit UTM Press 2017-09 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21739/1/Statistical%20optimization%20of%20gelatin%20immobilisation.pdf Nurhusna, Samsudin and Yumi Zuhanis Has-Yun, Hashim and Mohd Azmir, Arifin and Maizirwan, Mel and Hamzah, Mohd. Salleh and Norshariza, Nordin and Sopyan, I. and Dzun Noraini, Jimat (2017) Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility. Jurnal Teknologi (Sciences & Engineering), 79 (6). pp. 167-175. ISSN 2180–3722 https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/10417/6222 10.1111./jt.v79.10417
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Nurhusna, Samsudin Yumi Zuhanis Has-Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, I. Dzun Noraini, Jimat Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
description	Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier.
format	Article
author	Nurhusna, Samsudin Yumi Zuhanis Has-Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, I. Dzun Noraini, Jimat
author_facet	Nurhusna, Samsudin Yumi Zuhanis Has-Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, I. Dzun Noraini, Jimat
author_sort	Nurhusna, Samsudin
title	Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_short	Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_full	Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_fullStr	Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_full_unstemmed	Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_sort	statistical optimization of gelatin immobilisation on modified surface pcl microcarrier to improve pcl microcarrier compatibility
publisher	Penerbit UTM Press
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/1/Statistical%20optimization%20of%20gelatin%20immobilisation.pdf
first_indexed	2023-09-18T22:32:01Z
last_indexed	2023-09-18T22:32:01Z
_version_	1777416348822929408

Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility

Similar Items