UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231

UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231

Eleven compounds were identified during profiling of polyphenols by UPLC-QTOF/MS. In abundance was quercetin-3-O-α-l-arabinofuranoside in M. malabathricum ethanolic leaves extract while 6-hydroxykaempferol-3-O-glucoside was present in the leaves extract of M. decenfidum (its rare variety). TPC and T...

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Main Authors: Hazrulrizawati, Hamid, Aizi Nor Mazila, Ramli, Normaiza, Z., M. M., Yusoff
Format: Article
Language:English
Published: Elsevier Ltd 2018
Subjects:
RM Therapeutics. Pharmacology
Online Access:http://umpir.ump.edu.my/id/eprint/21567/
http://umpir.ump.edu.my/id/eprint/21567/
http://umpir.ump.edu.my/id/eprint/21567/
http://umpir.ump.edu.my/id/eprint/21567/7/UPLC-QTOFMS-based%20phenolic%20profiling-fist-2018-1.pdf
id ump-21567
recordtype eprints
spelling ump-215672018-07-26T07:45:10Z http://umpir.ump.edu.my/id/eprint/21567/ UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231 Hazrulrizawati, Hamid Aizi Nor Mazila, Ramli Normaiza, Z. M. M., Yusoff RM Therapeutics. Pharmacology Eleven compounds were identified during profiling of polyphenols by UPLC-QTOF/MS. In abundance was quercetin-3-O-α-l-arabinofuranoside in M. malabathricum ethanolic leaves extract while 6-hydroxykaempferol-3-O-glucoside was present in the leaves extract of M. decenfidum (its rare variety). TPC and TFC were significantly higher in M. decemfidum extract than M. malabathricum extract. During DPPH, FRAF and β-carotene bleaching assays, M. decemfidum extract exhibited greater antioxidant activity compared to M. malabathricum extract. Effect of M. malabathricum and M. decemfidum extracts on viability of MDA-MB-231 cell at concentrations 6.25–100 μg/mL were evaluated for 24, 48 and 72 h. After 48 and 72 h treatment, M. malabathricum and M. decemfidum leaves extracts exhibited significant activity in inhibiting MDA-MB-231 cancer cell line with M. malabathricum extract being more cytotoxic. M. malabathricum and M. imbricatum serves as potential daily dietary source of natural phenolics and to improve chemotherapeutic effectiveness. Elsevier Ltd 2018 Article PeerReviewed text en http://umpir.ump.edu.my/id/eprint/21567/7/UPLC-QTOFMS-based%20phenolic%20profiling-fist-2018-1.pdf Hazrulrizawati, Hamid and Aizi Nor Mazila, Ramli and Normaiza, Z. and M. M., Yusoff (2018) UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231. Food Chemistry, 265. pp. 253-259. ISSN 0308-8146 https://doi.org/10.1016/j.foodchem.2018.05.033 doi: 10.1016/j.foodchem.2018.05.033
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic RM Therapeutics. Pharmacology
spellingShingle RM Therapeutics. Pharmacology
Hazrulrizawati, Hamid
Aizi Nor Mazila, Ramli
Normaiza, Z.
M. M., Yusoff
UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231
description Eleven compounds were identified during profiling of polyphenols by UPLC-QTOF/MS. In abundance was quercetin-3-O-α-l-arabinofuranoside in M. malabathricum ethanolic leaves extract while 6-hydroxykaempferol-3-O-glucoside was present in the leaves extract of M. decenfidum (its rare variety). TPC and TFC were significantly higher in M. decemfidum extract than M. malabathricum extract. During DPPH, FRAF and β-carotene bleaching assays, M. decemfidum extract exhibited greater antioxidant activity compared to M. malabathricum extract. Effect of M. malabathricum and M. decemfidum extracts on viability of MDA-MB-231 cell at concentrations 6.25–100 μg/mL were evaluated for 24, 48 and 72 h. After 48 and 72 h treatment, M. malabathricum and M. decemfidum leaves extracts exhibited significant activity in inhibiting MDA-MB-231 cancer cell line with M. malabathricum extract being more cytotoxic. M. malabathricum and M. imbricatum serves as potential daily dietary source of natural phenolics and to improve chemotherapeutic effectiveness.
format Article
author Hazrulrizawati, Hamid
Aizi Nor Mazila, Ramli
Normaiza, Z.
M. M., Yusoff
author_facet Hazrulrizawati, Hamid
Aizi Nor Mazila, Ramli
Normaiza, Z.
M. M., Yusoff
author_sort Hazrulrizawati, Hamid
title UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231
title_short UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231
title_full UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231
title_fullStr UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231
title_full_unstemmed UPLC-QTOF/MS-based phenolic profiling of Melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell MDA-MB-231
title_sort uplc-qtof/ms-based phenolic profiling of melastomaceae, their antioxidant activity and cytotoxic effects against human breast cancer cell mda-mb-231
publisher Elsevier Ltd
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/21567/
http://umpir.ump.edu.my/id/eprint/21567/
http://umpir.ump.edu.my/id/eprint/21567/
http://umpir.ump.edu.my/id/eprint/21567/7/UPLC-QTOFMS-based%20phenolic%20profiling-fist-2018-1.pdf
first_indexed 2023-09-18T22:31:42Z
last_indexed 2023-09-18T22:31:42Z
_version_ 1777416328513060864

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