Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System

One of the big challenges in petrochemical industries is waste management. Currently, huge money was spending on the disposal of the waste. Industries are trying hard to find an alternative method to reduce the cost and improve the effectiveness of current waste management including treatme...

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Main Authors:	Abdul Aziz, Mohd Azoddein, F. A. M., Azli, A. B., Bustary, M. H., Sulaiman, A. Z. M., Salehen, Mazrul Nizam, Abu Seman, Said, N., M., Sulaiman
Format:	Article
Language:	English
Published:	IJRES 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/21438/ http://umpir.ump.edu.my/id/eprint/21438/ http://umpir.ump.edu.my/id/eprint/21438/1/Characterization%20of%20Enzyme%20Produced%20From%20Pseudomonas.pdf

id	ump-21438
recordtype	eprints
spelling	ump-214382018-06-29T02:59:50Z http://umpir.ump.edu.my/id/eprint/21438/ Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System Abdul Aziz, Mohd Azoddein F. A. M., Azli A. B., Bustary M. H., Sulaiman A. Z. M., Salehen Mazrul Nizam, Abu Seman Said, N. M., Sulaiman TP Chemical technology One of the big challenges in petrochemical industries is waste management. Currently, huge money was spending on the disposal of the waste. Industries are trying hard to find an alternative method to reduce the cost and improve the effectiveness of current waste management including treatment efficiency. Most of petrochemical wastes are containing benzene, toluene and xylene (BTX) which are very harmful to environment and living organisms. Common method used to separate the BTX from the waste are by using liquid-liquid and stripping process. One of the alternative to treat BTX is biological treatment method that used the natural capability of microorganisms to degrade to less harmful product is been applied. Some of examples are Pseudomonas Putida. (P. putida), Rhizobium, and Agrobacterium. P. putida is selected in this study for the biological treatment of BTX in petrochemical wastewater because it can produce an enzyme that has the capability of breakdown the aromatic hydrocarbon to carbon dioxide (CO2) and water (H2O). The main objective of this study is to produce and extract the enzymes produce, characterised the enzymes. This study also to investigate the effect of different concentration on the treatment as well as the growth of the bacteria. The enzyme is purified using salt precipitation and analysed using SDS_PAGE technique. UV-Vis is used to study the growth of the bacteria in the culture stock by measuring its optical density. The concentration of BTX was varied to determine the effect of the concentration on the percentage removal and the growth of P. putida. Enzymes detected or purified in this study was benzene reductase. Other expected enzymes were not able to be purified or analysed. It was found that in this experimental study, the removal of benzene is at 74% to 80%. The removal of toluene is at 62% to 75%. The removal of xylene is at 23% to 42%. Increasing the concentration of contaminants will reduce the removal capabilities. IJRES 2016 Article PeerReviewed text en http://umpir.ump.edu.my/id/eprint/21438/1/Characterization%20of%20Enzyme%20Produced%20From%20Pseudomonas.pdf Abdul Aziz, Mohd Azoddein and F. A. M., Azli and A. B., Bustary and M. H., Sulaiman and A. Z. M., Salehen and Mazrul Nizam, Abu Seman and Said, N. and M., Sulaiman (2016) Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System. International Journal of Research in Engineering and Science (IJRES), 4 (9). pp. 6-20. ISSN 2320-9364 http://ijres.org/papers/Volume%204/v4-i9/Version-1/C4911620.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abdul Aziz, Mohd Azoddein F. A. M., Azli A. B., Bustary M. H., Sulaiman A. Z. M., Salehen Mazrul Nizam, Abu Seman Said, N. M., Sulaiman Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System
description	One of the big challenges in petrochemical industries is waste management. Currently, huge money was spending on the disposal of the waste. Industries are trying hard to find an alternative method to reduce the cost and improve the effectiveness of current waste management including treatment efficiency. Most of petrochemical wastes are containing benzene, toluene and xylene (BTX) which are very harmful to environment and living organisms. Common method used to separate the BTX from the waste are by using liquid-liquid and stripping process. One of the alternative to treat BTX is biological treatment method that used the natural capability of microorganisms to degrade to less harmful product is been applied. Some of examples are Pseudomonas Putida. (P. putida), Rhizobium, and Agrobacterium. P. putida is selected in this study for the biological treatment of BTX in petrochemical wastewater because it can produce an enzyme that has the capability of breakdown the aromatic hydrocarbon to carbon dioxide (CO2) and water (H2O). The main objective of this study is to produce and extract the enzymes produce, characterised the enzymes. This study also to investigate the effect of different concentration on the treatment as well as the growth of the bacteria. The enzyme is purified using salt precipitation and analysed using SDS_PAGE technique. UV-Vis is used to study the growth of the bacteria in the culture stock by measuring its optical density. The concentration of BTX was varied to determine the effect of the concentration on the percentage removal and the growth of P. putida. Enzymes detected or purified in this study was benzene reductase. Other expected enzymes were not able to be purified or analysed. It was found that in this experimental study, the removal of benzene is at 74% to 80%. The removal of toluene is at 62% to 75%. The removal of xylene is at 23% to 42%. Increasing the concentration of contaminants will reduce the removal capabilities.
format	Article
author	Abdul Aziz, Mohd Azoddein F. A. M., Azli A. B., Bustary M. H., Sulaiman A. Z. M., Salehen Mazrul Nizam, Abu Seman Said, N. M., Sulaiman
author_facet	Abdul Aziz, Mohd Azoddein F. A. M., Azli A. B., Bustary M. H., Sulaiman A. Z. M., Salehen Mazrul Nizam, Abu Seman Said, N. M., Sulaiman
author_sort	Abdul Aziz, Mohd Azoddein
title	Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System
title_short	Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System
title_full	Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System
title_fullStr	Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System
title_full_unstemmed	Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System
title_sort	characterization of enzyme produced from pseudomonas putida for btx (benzene, toluene & xylene) treatment in petrochemical industry wastewater system
publisher	IJRES
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/21438/ http://umpir.ump.edu.my/id/eprint/21438/ http://umpir.ump.edu.my/id/eprint/21438/1/Characterization%20of%20Enzyme%20Produced%20From%20Pseudomonas.pdf
first_indexed	2023-09-18T22:31:27Z
last_indexed	2023-09-18T22:31:27Z
_version_	1777416313398886400

Characterization of Enzyme Produced from Pseudomonas Putida for BTX (Benzene, Toluene & Xylene) Treatment in Petrochemical Industry Wastewater System

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