Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design

Attempts to gain an insight and present bacterial growth pattern from OVAT perspectives are quite defective and strenuous. The present study aimed at evaluating this concept from the different perspective by evaluating factors with more impacts on the growth of a mes...

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Main Authors:	Ahmad, Mani Malam, Abd. Aziz, Mohd Azoddein, Mior Ahmad Khushairi, Mohd Zahari, Mazrul Nizam, Abu Seman, Mohammed Saedi, Jami
Format:	Article
Language:	English
Published:	SERSC 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/21393/ http://umpir.ump.edu.my/id/eprint/21393/ http://umpir.ump.edu.my/id/eprint/21393/1/Screening%20of%20Effective%20Markers%20for%20Mesophilic%20Bacterium%20Growth.pdf

id	ump-21393
recordtype	eprints
spelling	ump-213932018-06-26T05:26:45Z http://umpir.ump.edu.my/id/eprint/21393/ Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design Ahmad, Mani Malam Abd. Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammed Saedi, Jami TP Chemical technology Attempts to gain an insight and present bacterial growth pattern from OVAT perspectives are quite defective and strenuous. The present study aimed at evaluating this concept from the different perspective by evaluating factors with more impacts on the growth of a mesophilic bacterium, Bacillus cereus (ATCC 14579) in a batch type medium of orbital shake flasks. Screening of linear and interactions effects of parametric markers was evaluated by two level (24) factorial design of experiment (DOE). Growth was found to respond significantly to nutrient concentration coupled with other independent variables. The factorial models established from experimental design to investigate the individual and interactions effects toward the response within the selected variables of nutrient concentration (4-16g l-1), temperature (30 oC –42 oC), agitation (140 rpm-200 rpm) and acclimatization time (24 hours-72 hours). These were statistically validated using analysis of variance (ANOVA). The results revealed that the model terms were all significant with F-value of 251.07 at (p=0.004). The model term having the most significant effect on the response was nutrient concentration. And the magnitude of the influence is in the ascending order A > B> AB > C > AC. Based on the R2 and adjusted R2 the estimated model terms spell high degree of relationship between observed and predicted values, thus the prediction ability of the mo dels is maintained. It could, therefore, be concluded that nutrient concentration, temperature and acclimatization time were variables that greatly limit growth at a specific range. SERSC 2017 Article PeerReviewed text en http://umpir.ump.edu.my/id/eprint/21393/1/Screening%20of%20Effective%20Markers%20for%20Mesophilic%20Bacterium%20Growth.pdf Ahmad, Mani Malam and Abd. Aziz, Mohd Azoddein and Mior Ahmad Khushairi, Mohd Zahari and Mazrul Nizam, Abu Seman and Mohammed Saedi, Jami (2017) Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design. International Journal of Bio-Science and Bio-Technology (IJBSBT), 9 (3). pp. 59-74. ISSN 2233-7849 http://www.sersc.org/journals/IJBSBT/vol9_no3/6.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ahmad, Mani Malam Abd. Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammed Saedi, Jami Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design
description	Attempts to gain an insight and present bacterial growth pattern from OVAT perspectives are quite defective and strenuous. The present study aimed at evaluating this concept from the different perspective by evaluating factors with more impacts on the growth of a mesophilic bacterium, Bacillus cereus (ATCC 14579) in a batch type medium of orbital shake flasks. Screening of linear and interactions effects of parametric markers was evaluated by two level (24) factorial design of experiment (DOE). Growth was found to respond significantly to nutrient concentration coupled with other independent variables. The factorial models established from experimental design to investigate the individual and interactions effects toward the response within the selected variables of nutrient concentration (4-16g l-1), temperature (30 oC –42 oC), agitation (140 rpm-200 rpm) and acclimatization time (24 hours-72 hours). These were statistically validated using analysis of variance (ANOVA). The results revealed that the model terms were all significant with F-value of 251.07 at (p=0.004). The model term having the most significant effect on the response was nutrient concentration. And the magnitude of the influence is in the ascending order A > B> AB > C > AC. Based on the R2 and adjusted R2 the estimated model terms spell high degree of relationship between observed and predicted values, thus the prediction ability of the mo dels is maintained. It could, therefore, be concluded that nutrient concentration, temperature and acclimatization time were variables that greatly limit growth at a specific range.
format	Article
author	Ahmad, Mani Malam Abd. Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammed Saedi, Jami
author_facet	Ahmad, Mani Malam Abd. Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Mohammed Saedi, Jami
author_sort	Ahmad, Mani Malam
title	Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design
title_short	Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design
title_full	Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design
title_fullStr	Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design
title_full_unstemmed	Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design
title_sort	screening of effective markers for mesophilic bacterium growth using factorial experimental design
publisher	SERSC
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/21393/ http://umpir.ump.edu.my/id/eprint/21393/ http://umpir.ump.edu.my/id/eprint/21393/1/Screening%20of%20Effective%20Markers%20for%20Mesophilic%20Bacterium%20Growth.pdf
first_indexed	2023-09-18T22:31:21Z
last_indexed	2023-09-18T22:31:21Z
_version_	1777416307296174080

Screening of Effective Markers for Mesophilic Bacterium Growth Using Factorial Experimental Design

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