Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824

Biobutanol is an alternative energy that can be promising as the future energy source. It can be produced from natural and renewable agriculture wastes such as oil palm frond (OPF) juice by microbes. Clostridium acetobutylicum has the ability to ferment the sugars in OPF juice as carbon source into...

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Main Authors: Nur Syazana, Muhd Nasrah, Mior Ahmad Khushairi, Mohd Zahari, N., Masngut, Hidayah, Ariffin
Format: Article
Language:English
Published: Bangladesh University of Engineering and Technology 2017
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/20581/
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http://umpir.ump.edu.my/id/eprint/20581/
http://umpir.ump.edu.my/id/eprint/20581/1/Factorial%20Experimental%20Design%20For%20Biobutanol%20Production%20From%20Oil%20Palm%20Frond%20%28OPF%29%20Juice%20By%20Clostridium%20Acetobutylicum%20ATCC%20824.pdf
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spelling ump-205812018-02-23T06:38:55Z http://umpir.ump.edu.my/id/eprint/20581/ Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824 Nur Syazana, Muhd Nasrah Mior Ahmad Khushairi, Mohd Zahari N., Masngut Hidayah, Ariffin TP Chemical technology Biobutanol is an alternative energy that can be promising as the future energy source. It can be produced from natural and renewable agriculture wastes such as oil palm frond (OPF) juice by microbes. Clostridium acetobutylicum has the ability to ferment the sugars in OPF juice as carbon source into biobutanol. This research aimed to investigate the effect of independent and interaction factors; initial pH medium (5-7), inoculum size (1-20%), initial total sugars concentration (40-60 g/L), temperature (32-42°C) and yeast extract concentration (1-10 g/L) on the production of biobutanol from oil palm frond (OPF) juice by C. acetobutylicum ATCC 824 using a two level half factorial design which have been developed by the Design Expert Software Version 7.1. Based on the factorial analysis, it was observed that the most significant parameter was yeast extract concentration, which contributes 8.20%, followed by inoculum size and temperature, which were contribute 7.84% and 7.56%, respectively. The analysis showed the R2 value for the model was 0.9805 and the interaction between inoculum size and temperature gave the highest influenced to the fermentation process with contribution up to 16.31%. From the validation experiments, the experimental values were reasonable close to the predicted values with only 5.87% and 10.09% of errors. It confirmed the validity and adequacy of the predicted models. Hence, the data analysis developed from the Design Expert Software could reliably predict biobutanol yields. This study indicated that each of the factors may affect the fermentation process of the biobutanol production. Bangladesh University of Engineering and Technology 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/20581/1/Factorial%20Experimental%20Design%20For%20Biobutanol%20Production%20From%20Oil%20Palm%20Frond%20%28OPF%29%20Juice%20By%20Clostridium%20Acetobutylicum%20ATCC%20824.pdf Nur Syazana, Muhd Nasrah and Mior Ahmad Khushairi, Mohd Zahari and N., Masngut and Hidayah, Ariffin (2017) Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824. Chemical Engineering Research Bulletin, 19 (Special). pp. 36-42. ISSN 0379-7678 (Print); 2072-9510 (Online) https://www.banglajol.info/index.php/CERB/article/view/33774 DOI: http://dx.doi.org/10.3329/cerb.v19i0.33774
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nur Syazana, Muhd Nasrah
Mior Ahmad Khushairi, Mohd Zahari
N., Masngut
Hidayah, Ariffin
Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824
description Biobutanol is an alternative energy that can be promising as the future energy source. It can be produced from natural and renewable agriculture wastes such as oil palm frond (OPF) juice by microbes. Clostridium acetobutylicum has the ability to ferment the sugars in OPF juice as carbon source into biobutanol. This research aimed to investigate the effect of independent and interaction factors; initial pH medium (5-7), inoculum size (1-20%), initial total sugars concentration (40-60 g/L), temperature (32-42°C) and yeast extract concentration (1-10 g/L) on the production of biobutanol from oil palm frond (OPF) juice by C. acetobutylicum ATCC 824 using a two level half factorial design which have been developed by the Design Expert Software Version 7.1. Based on the factorial analysis, it was observed that the most significant parameter was yeast extract concentration, which contributes 8.20%, followed by inoculum size and temperature, which were contribute 7.84% and 7.56%, respectively. The analysis showed the R2 value for the model was 0.9805 and the interaction between inoculum size and temperature gave the highest influenced to the fermentation process with contribution up to 16.31%. From the validation experiments, the experimental values were reasonable close to the predicted values with only 5.87% and 10.09% of errors. It confirmed the validity and adequacy of the predicted models. Hence, the data analysis developed from the Design Expert Software could reliably predict biobutanol yields. This study indicated that each of the factors may affect the fermentation process of the biobutanol production.
format Article
author Nur Syazana, Muhd Nasrah
Mior Ahmad Khushairi, Mohd Zahari
N., Masngut
Hidayah, Ariffin
author_facet Nur Syazana, Muhd Nasrah
Mior Ahmad Khushairi, Mohd Zahari
N., Masngut
Hidayah, Ariffin
author_sort Nur Syazana, Muhd Nasrah
title Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824
title_short Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824
title_full Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824
title_fullStr Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824
title_full_unstemmed Factorial Experimental Design for Biobutanol Production from Oil Palm Frond (OPF) Juice by Clostridium Acetobutylicum ATCC 824
title_sort factorial experimental design for biobutanol production from oil palm frond (opf) juice by clostridium acetobutylicum atcc 824
publisher Bangladesh University of Engineering and Technology
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/20581/
http://umpir.ump.edu.my/id/eprint/20581/
http://umpir.ump.edu.my/id/eprint/20581/
http://umpir.ump.edu.my/id/eprint/20581/1/Factorial%20Experimental%20Design%20For%20Biobutanol%20Production%20From%20Oil%20Palm%20Frond%20%28OPF%29%20Juice%20By%20Clostridium%20Acetobutylicum%20ATCC%20824.pdf
first_indexed 2023-09-18T22:29:46Z
last_indexed 2023-09-18T22:29:46Z
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