Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus

Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus

Benzyl acetone, a carboxylic derivative constitute a considerable percentage of the overall yield of essential oil extracted from Aquilaria malaccensis (agarwood) and thus, considered as one of the major constituents of agarwood oil. This study aimed at isolating benzyl acetone from essential oil of...

Full description

Bibliographic Details
Main Author: Norul Amalina, Sabri
Format: Thesis
Language:English
English
English
Published: 2017
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/20522/
http://umpir.ump.edu.my/id/eprint/20522/
http://umpir.ump.edu.my/id/eprint/20522/1/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-Table%20of%20contents.PDF
http://umpir.ump.edu.my/id/eprint/20522/6/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-Abstract.PDF
http://umpir.ump.edu.my/id/eprint/20522/11/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-References.PDF
id ump-20522
recordtype eprints
spelling ump-205222018-02-28T03:44:25Z http://umpir.ump.edu.my/id/eprint/20522/ Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus Norul Amalina, Sabri TP Chemical technology Benzyl acetone, a carboxylic derivative constitute a considerable percentage of the overall yield of essential oil extracted from Aquilaria malaccensis (agarwood) and thus, considered as one of the major constituents of agarwood oil. This study aimed at isolating benzyl acetone from essential oil of A. malaccensis using preparative GC (prep-GC); studying the fate of benzyl acetone when transformed using selected microorganisms commonly found in environment; developing efficient biotransformation system by optimizing parameters namely initial substrate and glucose concentration, initial pH and temperature of reaction. Essential oil of A. malaccensis was extracted using Microwave-Assisted Extraction (MAE) and preparative GC (prep-GC) was used to isolate benzyl acetone. Identity of isolated benzyl acetone was confirmed by comparing the peak to that of commercial benzyl acetone using GC-MS. The whole cells of Saccharomyces cerevisiae, Aspergillus niger, Pseudomonas aeruginosa, Enterococcusfaecalis and Bacillus cereus were used for biotransformation of commercial benzyl acetone in this study. GC-MS was used to identify and quantify biotransformation product. Reduction of benzyl acetone to 4-phenyl-2-butanol occurred in all experiments using these five microorganisms. The optimum condition for the highest biotransformation activity using S. cerevisiae: 34.01 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.5 and 30°C; A. niger: 94.33 % conversion in 0.15 g/L benzyl acetone, 50 g/L glucose, pH 7.5 and 27°C; P. aeruginosa: 98.27 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.0 and 30°C; E.faecalis: 7.17 % conversion in 0.15 g/L benzyl acetone, 40 g/L glucose, pH 7.0 and 37°C; B. cereus: 9.84 % conversion in 0.15 gIL, 40 g/L glucose, pH 7.0 and 40°C. Biotransformation of benzyl acetone resulted in formation of 4-phenyl-2-butanol which is not naturally found in agarwood oil. P. aeruginosa was proven to be the most efficient biocatalyst for biotransformation of benzyl acetone to 4-phenyl-2-butanol, followed by A. niger, S. cerevisiae, E. faecalis and B. cereus. 2017 Thesis NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/20522/1/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-Table%20of%20contents.PDF application/pdf en http://umpir.ump.edu.my/id/eprint/20522/6/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-Abstract.PDF application/pdf en http://umpir.ump.edu.my/id/eprint/20522/11/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-References.PDF Norul Amalina, Sabri (2017) Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus. Masters thesis, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:99687&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Norul Amalina, Sabri
Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
description Benzyl acetone, a carboxylic derivative constitute a considerable percentage of the overall yield of essential oil extracted from Aquilaria malaccensis (agarwood) and thus, considered as one of the major constituents of agarwood oil. This study aimed at isolating benzyl acetone from essential oil of A. malaccensis using preparative GC (prep-GC); studying the fate of benzyl acetone when transformed using selected microorganisms commonly found in environment; developing efficient biotransformation system by optimizing parameters namely initial substrate and glucose concentration, initial pH and temperature of reaction. Essential oil of A. malaccensis was extracted using Microwave-Assisted Extraction (MAE) and preparative GC (prep-GC) was used to isolate benzyl acetone. Identity of isolated benzyl acetone was confirmed by comparing the peak to that of commercial benzyl acetone using GC-MS. The whole cells of Saccharomyces cerevisiae, Aspergillus niger, Pseudomonas aeruginosa, Enterococcusfaecalis and Bacillus cereus were used for biotransformation of commercial benzyl acetone in this study. GC-MS was used to identify and quantify biotransformation product. Reduction of benzyl acetone to 4-phenyl-2-butanol occurred in all experiments using these five microorganisms. The optimum condition for the highest biotransformation activity using S. cerevisiae: 34.01 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.5 and 30°C; A. niger: 94.33 % conversion in 0.15 g/L benzyl acetone, 50 g/L glucose, pH 7.5 and 27°C; P. aeruginosa: 98.27 % conversion in 0.1 g/L benzyl acetone, 20 g/L glucose, pH 7.0 and 30°C; E.faecalis: 7.17 % conversion in 0.15 g/L benzyl acetone, 40 g/L glucose, pH 7.0 and 37°C; B. cereus: 9.84 % conversion in 0.15 gIL, 40 g/L glucose, pH 7.0 and 40°C. Biotransformation of benzyl acetone resulted in formation of 4-phenyl-2-butanol which is not naturally found in agarwood oil. P. aeruginosa was proven to be the most efficient biocatalyst for biotransformation of benzyl acetone to 4-phenyl-2-butanol, followed by A. niger, S. cerevisiae, E. faecalis and B. cereus.
format Thesis
author Norul Amalina, Sabri
author_facet Norul Amalina, Sabri
author_sort Norul Amalina, Sabri
title Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_short Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_full Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_fullStr Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_full_unstemmed Biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
title_sort biotransformation of benzyl acetone using saccharomyces cerevisiae, aspergillus niger, pseudomonas aeruginosa, enterococcus faecalis and bacillus cereus
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/20522/
http://umpir.ump.edu.my/id/eprint/20522/
http://umpir.ump.edu.my/id/eprint/20522/1/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-Table%20of%20contents.PDF
http://umpir.ump.edu.my/id/eprint/20522/6/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-Abstract.PDF
http://umpir.ump.edu.my/id/eprint/20522/11/Biotransformation%20of%20benzyl%20acetone%20using%20saccharomyces%20cerevisiae%2C%20aspergillus%20niger%2C%20pseudomonas%20aeruginosa%2C%20enterococcus%20faecalis%20and%20bacillus%20cereus-References.PDF
first_indexed 2023-09-18T22:29:38Z
last_indexed 2023-09-18T22:29:38Z
_version_ 1777416199307526144

Similar Items