Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities

The present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) of Cheilanthes tenuifolia. Flavonoids derived from C. tenuifolia (fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-...

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Main Authors:	Jarial, Rini, Shard, A., Thakur, Sveta, Mimi Sakinah, A. M., Zularisam, A. W., Rezania, Shahabaldin, Kanwar, S. S., Singh, L.
Format:	Article
Language:	English
Published:	King Saud University 2017
Subjects:	TD Environmental technology. Sanitary engineering
Online Access:	http://umpir.ump.edu.my/id/eprint/20058/ http://umpir.ump.edu.my/id/eprint/20058/ http://umpir.ump.edu.my/id/eprint/20058/ http://umpir.ump.edu.my/id/eprint/20058/1/CHARACTERIZATION%20FRONT%20PG.pdf

id	ump-20058
recordtype	eprints
spelling	ump-200582019-10-03T07:50:48Z http://umpir.ump.edu.my/id/eprint/20058/ Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities Jarial, Rini Shard, A. Thakur, Sveta Mimi Sakinah, A. M. Zularisam, A. W. Rezania, Shahabaldin Kanwar, S. S. Singh, L. TD Environmental technology. Sanitary engineering The present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) of Cheilanthes tenuifolia. Flavonoids derived from C. tenuifolia (fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-preventive potential against selected bacterial panel. A preparative column chromatography with a column Sephadex LH-20 was used to isolate and purify flavonoids from C. tenuifolia. Their structure and chemical bonds were identified by using Nuclear Magnetic Resonance (NMR) spectroscopy and Fourier Transform-Infra Red Spectroscopy (FTIR). Two flavonoids were identified as rutin (2.8 mg) and quercetin (3.34 mg) from 100 g of C. tenuifolia. The minimum inhibitory concentration (MIC) values of (2.25 and 0.45 mg/ml) were obtained in purified flavonoids against Staphylococcus aureus and Enterobacter sp., respectively. The two flavonoids (Rutin and quercetin) showed significant in vitro anti-oxidant activity; in this regard, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging potential of quercetin (86.1%) was higher than that of rutin (73.2%). However, for human hepatoma HepG2 and human carcinoma HeLa cells, quercetin exhibited high anti-cancer activity than rutin. The purified compounds (rutin and quercetin) with having great potential of free radical inactivator in HepG2 cells. The results suggest that MFE of C. tenuifolia could potentially be employed in traditional medicine as they are rich in compounds with anti-oxidant, anti- microbial and anti-cancer properties. King Saud University 2017 Article PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/20058/1/CHARACTERIZATION%20FRONT%20PG.pdf Jarial, Rini and Shard, A. and Thakur, Sveta and Mimi Sakinah, A. M. and Zularisam, A. W. and Rezania, Shahabaldin and Kanwar, S. S. and Singh, L. (2017) Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities. Journal of King Saud University - Science. ISSN 1018-3647 (In Press) http://dx.doi.org/10.1016/j.jksus.2017.04.007 doi:10.1016/j.jksus.2017.04.007
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TD Environmental technology. Sanitary engineering
spellingShingle	TD Environmental technology. Sanitary engineering Jarial, Rini Shard, A. Thakur, Sveta Mimi Sakinah, A. M. Zularisam, A. W. Rezania, Shahabaldin Kanwar, S. S. Singh, L. Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities
description	The present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) of Cheilanthes tenuifolia. Flavonoids derived from C. tenuifolia (fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-preventive potential against selected bacterial panel. A preparative column chromatography with a column Sephadex LH-20 was used to isolate and purify flavonoids from C. tenuifolia. Their structure and chemical bonds were identified by using Nuclear Magnetic Resonance (NMR) spectroscopy and Fourier Transform-Infra Red Spectroscopy (FTIR). Two flavonoids were identified as rutin (2.8 mg) and quercetin (3.34 mg) from 100 g of C. tenuifolia. The minimum inhibitory concentration (MIC) values of (2.25 and 0.45 mg/ml) were obtained in purified flavonoids against Staphylococcus aureus and Enterobacter sp., respectively. The two flavonoids (Rutin and quercetin) showed significant in vitro anti-oxidant activity; in this regard, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging potential of quercetin (86.1%) was higher than that of rutin (73.2%). However, for human hepatoma HepG2 and human carcinoma HeLa cells, quercetin exhibited high anti-cancer activity than rutin. The purified compounds (rutin and quercetin) with having great potential of free radical inactivator in HepG2 cells. The results suggest that MFE of C. tenuifolia could potentially be employed in traditional medicine as they are rich in compounds with anti-oxidant, anti- microbial and anti-cancer properties.
format	Article
author	Jarial, Rini Shard, A. Thakur, Sveta Mimi Sakinah, A. M. Zularisam, A. W. Rezania, Shahabaldin Kanwar, S. S. Singh, L.
author_facet	Jarial, Rini Shard, A. Thakur, Sveta Mimi Sakinah, A. M. Zularisam, A. W. Rezania, Shahabaldin Kanwar, S. S. Singh, L.
author_sort	Jarial, Rini
title	Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities
title_short	Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities
title_full	Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities
title_fullStr	Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities
title_full_unstemmed	Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities
title_sort	characterization of flavonoids from fern cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities
publisher	King Saud University
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/20058/ http://umpir.ump.edu.my/id/eprint/20058/ http://umpir.ump.edu.my/id/eprint/20058/ http://umpir.ump.edu.my/id/eprint/20058/1/CHARACTERIZATION%20FRONT%20PG.pdf
first_indexed	2023-09-18T22:28:43Z
last_indexed	2023-09-18T22:28:43Z
_version_	1777416141745946624

Characterization of Flavonoids from Fern Cheilanthes Tenuifolia and Evaluation of Antioxidant, Antimicrobial and Anticancer Activities

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