Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria

Environmental pollution is a major problem in the developed and developing countries. Fish fins (FF) and chicken feathers (CF) can be sources of solid waste contamination; hence, both FF and CF were investigated for alkaline protease (AP) production through microbial degradation. Proteins were extra...

Full description

Bibliographic Details
Main Authors:	Thanoon, Raid D., Subramaniam, Rubaaini, Makky, Essam A., M. M., Yusoff
Format:	Article
Language:	English
Published:	JJBS 2018
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/19861/ http://umpir.ump.edu.my/id/eprint/19861/ http://umpir.ump.edu.my/id/eprint/19861/7/Utilization%20of%20Extracted%20Protein%20from%20Fish%20Fin%20and%20Chicken-fist-2018.pdf

id	ump-19861
recordtype	eprints
spelling	ump-198612018-09-27T08:44:44Z http://umpir.ump.edu.my/id/eprint/19861/ Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria Thanoon, Raid D. Subramaniam, Rubaaini Makky, Essam A. M. M., Yusoff Q Science (General) QR Microbiology Environmental pollution is a major problem in the developed and developing countries. Fish fins (FF) and chicken feathers (CF) can be sources of solid waste contamination; hence, both FF and CF were investigated for alkaline protease (AP) production through microbial degradation. Proteins were extracted from these samples and added into the production medium containing the bacterial suspension, and assayed for AP production. The process parameters were optimized by OFAT and the optimum conditions for CF and FF were pH 9.0, temperature of 28°C for CF and 40°C for FF, incubation period was 6 and 10 days for CF and FF, respectively. The optimum carbon source was galactose and glucose for CF and FF respectively, and the optimum nitrogen source was ammonium chloride and beef extract, respectively. The inoculum size of 1.5 mL and a protein volume of 0.5 and 2.0 mL for CF and FF, respectively was recorded. The present study indicates the protein was successfully extracted from the waste used and degraded by AP enzyme that produced and optimized using OFAT by indigenous bacterial isolates. JJBS 2018 Article PeerReviewed application/pdf en cc_by_nc_sa http://umpir.ump.edu.my/id/eprint/19861/7/Utilization%20of%20Extracted%20Protein%20from%20Fish%20Fin%20and%20Chicken-fist-2018.pdf Thanoon, Raid D. and Subramaniam, Rubaaini and Makky, Essam A. and M. M., Yusoff (2018) Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria. Jordan Journal of Biological Sciences, 11 (1). pp. 17-22. ISSN 2307-7166 http://www.jjbs.hu.edu.jo/files/v11n1/Paper%20Number%203m.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Thanoon, Raid D. Subramaniam, Rubaaini Makky, Essam A. M. M., Yusoff Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
description	Environmental pollution is a major problem in the developed and developing countries. Fish fins (FF) and chicken feathers (CF) can be sources of solid waste contamination; hence, both FF and CF were investigated for alkaline protease (AP) production through microbial degradation. Proteins were extracted from these samples and added into the production medium containing the bacterial suspension, and assayed for AP production. The process parameters were optimized by OFAT and the optimum conditions for CF and FF were pH 9.0, temperature of 28°C for CF and 40°C for FF, incubation period was 6 and 10 days for CF and FF, respectively. The optimum carbon source was galactose and glucose for CF and FF respectively, and the optimum nitrogen source was ammonium chloride and beef extract, respectively. The inoculum size of 1.5 mL and a protein volume of 0.5 and 2.0 mL for CF and FF, respectively was recorded. The present study indicates the protein was successfully extracted from the waste used and degraded by AP enzyme that produced and optimized using OFAT by indigenous bacterial isolates.
format	Article
author	Thanoon, Raid D. Subramaniam, Rubaaini Makky, Essam A. M. M., Yusoff
author_facet	Thanoon, Raid D. Subramaniam, Rubaaini Makky, Essam A. M. M., Yusoff
author_sort	Thanoon, Raid D.
title	Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
title_short	Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
title_full	Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
title_fullStr	Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
title_full_unstemmed	Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
title_sort	utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria
publisher	JJBS
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/19861/ http://umpir.ump.edu.my/id/eprint/19861/ http://umpir.ump.edu.my/id/eprint/19861/7/Utilization%20of%20Extracted%20Protein%20from%20Fish%20Fin%20and%20Chicken-fist-2018.pdf
first_indexed	2023-09-18T22:28:28Z
last_indexed	2023-09-18T22:28:28Z
_version_	1777416125668130816

Utilization of extracted protein from fish fin and chicken feather waste for alkaline protease production by indigenous bacteria

Similar Items